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I have another question in regards to my HIV test research. The rapid tests like Orasures Oraquick contains a strip of synthetic peptides that are used to represent proteins found in the envelope region of the virus. The test itself uses a solid phase where the antigens are located so that they can be immobilized. My question is, is when these immobilized antigens come into contact with the reagent inside of the test, can they become unimmobilized and travel through the test? Or are they permanently placed in the solid phase? Any info would be warmly appreciated!

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Whilst I don't know the specifics of the test you mention, I'd be very surprised if they could move after being fixed into place. Otherwise the line indicating a positive response may not appear due to diffusion of the antigens away from the line they are fixed into. – Rory M♦ Dec 26 '12 at 19:32

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