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Please would somebody help me to solve the following exercise: Determine the protein concentrations of the four unknown samples (A,B,C,D) using the raw data obtained from a Bradford assay.

Absorbance values at 595 nm for the BSA known samples and unknown samples are available. 40 μL of each protein sample was mixed with 2.96 mL of Bradford reagent. 

Sample          Absorbance @ 595nm
  A                     0.085
  B                     0.001
  C                     1.300
  D                     0.150

 BSA  mcg/ml          AU 595nm
     30                 0.04
     50                 0.05
     70                 0.09
     90                 0.11
    110                 0.14
    130                 0.17
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Hello there. This looks like a homework problem. Before one of us helps you, what have you tried so far? – leonardo Dec 30 '12 at 21:59
We expect some effort to solve the question yourself for homework questions, if you add what you have tried the question can be reopened. – Mad Scientist♦ Jan 2 at 10:05

closed as too localized by Mad Scientist♦ Jan 2 at 10:05

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