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What depth penetration can one typically get with atomic force microscopes? (i.e. how far into the tissue can one image?). I have found numbers on acquisition times (minutes) and spatial resolutions (~nm), but what about depth?

Also, what is the sensitivity of AFM? (e.g. in moles)

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I was under the impression that AFM works superficially on specimens. – user560 Jan 3 '13 at 1:20
up vote 4 down vote accepted

Taken right from the Wiki page you linked to:

AFM only images the surface of a specimen, to a maximum depth of 10-20 µm and a maximum scan area of 150 µm x 150 µm. Compared with scanning electron microscopy, SEM has a much larger depth of penetration and scanning area (~1 order of magnitude greater). AFM is also a much slower scanning method.

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