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I want to compare the brain sizes of two populations of fish. However, all the samples I have were fixed in 95% ethanol. As far as I know, 4% formalin is the normal fixative for soft tissues. Can I conduct my study, or do I need to obtain samples in formalin? I've scoured the literature, and I can't find any useful recommendations.

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Are all the samples fixed in ethanol? Or some in ethanol and some in formalin? –  kmm Jan 21 '13 at 22:33

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