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Is there a protein that catalyzes this or does it happen spontaneously?

In which part of the cell does this happen?

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The process to which you refer is called tRNA charging and is catalyzed in the cytosol by a class of enzymes called aminoacyl tRNA synthetases.

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...and it happens in the cytosol. – Alan Boyd Feb 28 at 21:45
@ohcanada, sorry for blowing off your suggested edit. I should have given the original question and your suggestion more consideration. – Daniel Standage Mar 1 at 2:33

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