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Is there a protein that catalyzes this or does it happen spontaneously?

In which part of the cell does this happen?

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up vote 4 down vote accepted

The process to which you refer is called tRNA charging and is catalyzed in the cytosol by a class of enzymes called aminoacyl tRNA synthetases.

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...and it happens in the cytosol. –  Alan Boyd Feb 28 '13 at 21:45
    
@ohcanada, sorry for blowing off your suggested edit. I should have given the original question and your suggestion more consideration. –  Daniel Standage Mar 1 '13 at 2:33
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