Can I use PCR buffer instead of cDNA synthesis buffer?

Question

I am using Fermentase First strand cDNA synthesis kit but its buffer is over. I need to work today but I have not access to any sorce currently. I have PCR buffer in the lab. Can I use PCR buffer instead of cDNA synthesis buffer? What are your other suggestions? Thank you

Answer 1

This page has the recipe for 10X M-MuLV RT buffer:

• 500 mM Tris-HCl
• 750 mM KCl
• 30 mM MgCl2
• 100 mM DTT
• pH 8.3

If you have the basic reagents, I suggest making that.