I am using Fermentase First strand cDNA synthesis kit but its buffer is over. I need to work today but I have not access to any sorce currently. I have PCR buffer in the lab. Can I use PCR buffer instead of cDNA synthesis buffer? What are your other suggestions? Thank you
This page has the recipe for 10X M-MuLV RT buffer:
If you have the basic reagents, I suggest making that.