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I am using Fermentase First strand cDNA synthesis kit but its buffer is over. I need to work today but I have not access to any sorce currently. I have PCR buffer in the lab. Can I use PCR buffer instead of cDNA synthesis buffer? What are your other suggestions? Thank you

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1 Answer 1

up vote 6 down vote accepted

This page has the recipe for 10X M-MuLV RT buffer:

  • 500 mM Tris-HCl
  • 750 mM KCl
  • 30 mM MgCl2
  • 100 mM DTT
  • pH 8.3

If you have the basic reagents, I suggest making that.

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