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I know the reaction proceeds as follows:

Pyruvate accepts the electrons from NADH to form lactate. What would be the intermediate in this reaction? Would it be NADH or NAD+?

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While the topic is certainly a biomolecule, I would argue that the actual required knowledge to answer this is more likely to be found on Chemistry.SE. – Armatus Mar 11 at 23:46
Btw since the reaction is only a single electron movement, I think there might not be any intermediate? But as said above, chemistry knowledge like this might be limited on this site (mine definitely is). – Armatus Mar 11 at 23:50
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This is a biochemistry question, and I don't think it should be closed. However I don't understand the question. Does the cell-free extract contain mitochondria? If so the pyruvate will enter and be converted to acetyl CoA by pyruvate dehydrogenase. The text of the question makes no sense to me. What do you mean by "intermediate" in this context? – Alan Boyd Mar 12 at 7:52
N.b for the closure this has been cross posted to chemistry.se rather than necessarily being completely off topic. – Rory M♦ Mar 12 at 11:31

closed as off topic by Armatus, Rory M♦ Mar 12 at 11:30

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