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We have to measure the protocol of electric shocking mice. The mice are head-fixed, we have to guarantee that the electric current is significant to make the mice learn, but not too big to make the mice violently struggle and break their skull bone. In order to measure the proper electric current value, the simple way to increase the current step by step until the mice break their skull bone. But we think this way is not acceptable by the experimental ethics.

What's a better way? Thanks!

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Look in the literature maybe? Why do you need them head fixed? –  nico Mar 26 '13 at 7:10
    
Hi @nico , obviously I need to observe their brain in vivo:-) –  Skyler Mar 26 '13 at 8:01
    
well, if you're doing electrophysiology maybe you could chronically implant them. For imaging it gets complicated... –  nico Mar 26 '13 at 10:24
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Talk to the vets that will evaluate your protocol. They will have experience and ideas. –  kmm Mar 26 '13 at 15:57
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@Skyler: One option is presented in Stroh et al 2013 although it sounds really tricky (especially seen the names of the two last authors!) –  nico Apr 7 '13 at 9:55
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