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I just saw this paper.

A Highly Efficient Molecular Cloning Platform that Utilises a Small Bacterial Toxin Gene

I was curious if anyone had experience with this cloning strategy using the IbsC toxin.

(edit), dd3 makes a good point in specifying this question. I'm particularly curious about the efficiency of the technique and what major downsides and false positives that may arise. Furthermore, how long does the protocol actually take?

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closed as unclear what you're asking by Chris, Chris Stronks, Bez, Good Gravy, Superbest Dec 10 at 2:55

Please clarify your specific problem or add additional details to highlight exactly what you need. As it's currently written, it’s hard to tell exactly what you're asking. See the How to Ask page for help clarifying this question.If this question can be reworded to fit the rules in the help center, please edit the question.

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I don't, but when you ask if anyone has experience, what sort of questions would you like them to answer? For example, do you want to know how efficient it is compared to a particular cloning strategy? –  dd3 Mar 29 '13 at 22:08
    
I think you have a legitimate, good question, but since open ended questions are discouraged, you should revise it to be compatible with the format. What exactly is your question? What about our experience do you want to know? Is it just the efficiency and time? I would be happy to reopen/upvote once these are addressed. –  Superbest Dec 10 at 2:57