I am conducting a mutagenesis on a gene in vivo of which I need to ligate into an expression vector. The primers I have designed overlap restriction sites of which I plan to use to ligate into the expression vector. The parts between the primers I will try to create mutants with error prone PCR.
My concern is the 3' A overhangs that are created from Taq polymerase. How prevalent is this? If it is highly common, what is the best way to account for these additions when trying to ligate my PCR product into the vector?