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We're producing some membrane proteins and they aren't amenable to freeze thaws even when we add glycerol. The proteins are solubilized in detergent above the cmc so they should be in micelle form in solution. Currently, they are being held at 4C. I'm curious what is the best way to store them for long term use?

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this sounds like a case by case issue,but i'd be interested to see what someone with experience would say. –  shigeta Apr 6 '13 at 3:35
    
Can't you just store the cells you use to produce the protein and thaw them when you need them to produce more? Like that you wouldn't need to store the protein itself for a longer time. –  suvidu Apr 8 '13 at 11:18
    
You could always just add sodium azide and keep them at +4, so they don't have to go through a freeze/thaw at all. –  MattDMo Apr 8 '13 at 15:32
    
@Suvidu, I would counter that if the purification protocol is a pain, it's a valuable investment to be able to scaleup effectively. –  bobthejoe Apr 9 '13 at 10:21
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