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I wonder if I can heat Trizol reagent for 30 min 65C. The goal is to disrupt protein-RNA complex while inhibiting nucleases. (I can't use RNasin cause it's inactivated in 65C, and can't use RVC cause reaction contains EDTA). I realize that heating Trizol is not safe and its vapors are hazardous, but i can perform it in the hood.

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As far as I know, Trizol disrupts protein-RNA complexes on its own - check the protocol. Also, phenol, a major component, is flammable. I don't know what the flash point of Trizol is, but I would be extremely careful. This protocol claims you can heat it. Does your experiment not work at RT? –  MattDMo Apr 8 '13 at 15:46
    
Heating wont be a nice idea. But if your cells are too resistant to lysis then you can keep the tubes in a warm water bath. However, bring it back to RT before adding chloroform. BTW what sample is this? –  WYSIWYG Apr 8 '13 at 16:08
    
If you are worried about safety, contact your university's Environmental Health and Safety office (or the equivalent). They can (should, and will) advise you on safety issues. –  kmm Apr 9 '13 at 0:46
    
What kind of cells or complexes do you have? I have worked a lot with Phenol based reagents but never found problems to work with it at room temperature. Besides that Phenol is volatile, especially at higher temperatures. You will have to do that in a good fume hood, otherwise this is a serious health issue. And definitely cool down before adding Chloroform. –  Chris Jan 18 at 9:56
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