I wonder if I can heat Trizol reagent for 30 min 65C. The goal is to disrupt protein-RNA complex while inhibiting nucleases. (I can't use RNasin cause it's inactivated in 65C, and can't use RVC cause reaction contains EDTA). I realize that heating Trizol is not safe and its vapors are hazardous, but i can perform it in the hood.
Working with hot phenolic solutions is rarely been done due to the dangers of this. Phenol needs to be handled in the fume hood anyway, at higher temperatures it gets even more volatile. So if you plan to do this, do it with extra care to avoid injury.
There is one paper that describes this method for bacteria, but this should work for other samples as well: "Evaluation of isolation methods and RNA integrity for bacterial RNA quantitation". However, there is another method, which was developed for extracting RNA from plant material infected by bacteria which is also worth a try (although it might need some adaptation), which works without hot phenol: "An optimized method for the extraction of bacterial mRNA from plant roots infected with Escherichia coli O157:H7".