Take the 2-minute tour ×
Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. It's 100% free, no registration required.

5,5'-dibromo-4,4'-dichloro-indigo is the product of X-gal cleavage, often used as a reporter with B-galactosidase. I've made the (unreferenced) observation that it seems as though the blue product slowly degrades over periods of time within the cell, allowing for cells to lose their blue coloration.

What mechanisms can explain the degradation of 5,5'-dibromo-4,4'-dichloro-indigo within cells?

share|improve this question
Can be 3 reasons: 1. Dilution 2. Reductive conversion to colorless leuco-indigo 3. Metabolic decay: I suspect the metabolism would be similar to that of purines –  WYSIWYG May 7 '13 at 17:56
^ that was purely speculation –  WYSIWYG May 7 '13 at 18:07
add comment

Your Answer


By posting your answer, you agree to the privacy policy and terms of service.

Browse other questions tagged or ask your own question.