I want to save my bacillus strains but I don't have access to a -80 degree freezer. What are possible alternatives?
Use agar stubs: pour some Nutrient agar (or any suitable agar medium) in a test tube (~10% of the tube volume) and punch your inoculum halfway into it using a pipette tip or inoculation loop. Store it in 4⁰C.
Glycerol stocks can be made and stored in -20⁰C also.