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I'm working with HEK293 cells, which are very easy to transfect. Does anyone have other suggestions for cell lines with very high transfection efficiency? Ideally the chromosomes would also be diploid (293 cells are aneuploid) and the cells would be easy to grow from single cells (293 cells do not like to be isolated).

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good luck finding truly diploid cell lines - unless you're working with primary cells, most cell lines are transformed, and have all sorts of genetic anomalies. –  MattDMo May 21 '13 at 12:25

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