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Is it possible to reliably isolate and amplify DNA from individual sperm and/or eggs (from a fish)? I'd imagine that the small amount of DNA would make the PCR a bit wacky. We've considered irradiating sperm and attempting to create haploid females via gynogenesis, but I was wondering if there was a faster and easier method that could utilize gametes directly.

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Are you limited to a particular species of fish? Or can you use one of the asexually-reproducing species? – Atticus29 Feb 5 at 7:57

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