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I want to experimentally look at the behavior of antibodies. To do so, I need to be able to bind these antibodies to a substrate. Does anybody know of a good substrate to use that antibodies bind to?

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Can you be more specific? What exactly do you want to study and how do you plan to do it? –  dd3 Jun 5 '13 at 22:37
    
I don't think jeffrey knows the "how" yet, but knowing "what" will help us to give more appropraite answers. –  leonardo Jun 7 '13 at 2:16
    
Hi thanks for your responses, I first want to study the specificity of antibodies to know to what substrates they bind. After that, I want to study the biological activity of the antibody over time.. but first I need to know: to what material antibodies bind and why and then try to observe that it works. Can anybody help with this? –  jeffrey Jun 7 '13 at 8:55
    
@jeffrey what is the source of the antibodies? Do you have purified material, or antiserum, or something in between? You refer to "the antibody" - it is difficult to imagine a situation where you would have pure antibody but not already know what the antigen is that it binds to, or an antiserum where you don't know what you expect it to react with - I think that you need to be much more specific before you get the help that you are seeking. –  Alan Boyd Jun 7 '13 at 11:50
    
Sorry can't tell I am not a biologist. The thing I need to know: does e.g. carbon attract these antibodies? And what chemical would repel them? –  jeffrey Jun 7 '13 at 22:59

1 Answer 1

Like any other protein, antibodies will aspecifically bind nitrocellulose or PVDF membranes, but any other protein present in your antibody preparation will also do.

Depending on the antibody class, more specific binding can be obtained with protein A or protein G, that recognize the Fc domain. It's usual to have protein A/G immobilized on a stationary phase like agarose.

If you know the antigen used to develop the antibody, you can also use the immunizing peptide crosslinked to CnBr activated sepharose as a probe to bind your antibodies.

Knowing something more about your antibody preparation and what exactly is the 'behavior' your want to study could help obtain better answers.

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