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I'm learning about microarrays and understand that an oligonucleotide can be immobilized to a solid like a CD acrylic plastic by modifying the oligo. Does anyone have a good protocol for modifying a normal oligo to one that can be immobilized like that?

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I suspect that this will rely upon incorporating a special group such as biotin into the oligo during synthesis. It is difficult to imagine a chemistry that would be limited to one of the ends of an existing oligo. –  Alan Boyd Jul 5 '13 at 9:39
    
In microarrays they convert mRNA to cDNA and then modifying the cDNA to be immobilized, so i think it's possible to modify an existing oligo without do it during synthesis. youtube.com/watch?v=VNsThMNjKhM –  Đức UltraSoft Jul 5 '13 at 10:48
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