Take the 2-minute tour ×
Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. It's 100% free, no registration required.

I'm learning about microarrays and understand that an oligonucleotide can be immobilized to a solid like a CD acrylic plastic by modifying the oligo. Does anyone have a good protocol for modifying a normal oligo to one that can be immobilized like that?

share|improve this question
I suspect that this will rely upon incorporating a special group such as biotin into the oligo during synthesis. It is difficult to imagine a chemistry that would be limited to one of the ends of an existing oligo. –  Alan Boyd Jul 5 '13 at 9:39
In microarrays they convert mRNA to cDNA and then modifying the cDNA to be immobilized, so i think it's possible to modify an existing oligo without do it during synthesis. youtube.com/watch?v=VNsThMNjKhM –  Đức UltraSoft Jul 5 '13 at 10:48

Your Answer


By posting your answer, you agree to the privacy policy and terms of service.

Browse other questions tagged or ask your own question.