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I did an expression profiling from publicly available RNAseq data for mouse tissues. While for liver and testes I am getting expected proteins in the top highly expressed mRNAs, for brain I am getting ferritin (Ferritin heavy chain 1: Fth1) as the top expressed mRNA!

There are a few studies which report elevated ferritin and iron in neurodegenerative diseases while some others have implicated its role in ageing.

I haven't come across any study on iron metabolism in the normal brain. Does iron metabolism play an important role in the function of the brain?

PS :

  • The data that I am talking about, has the SRA accession number- SRR652247 (I tried all three runs submitted in SRA)
  • The mice used in the experiment were 11 weeks old
  • I used bowtie -> eXpress for quantification of RNAseq reads.
share|improve this question
Look at the study summary - it describes expression profiling in testis. Do you think they might have mis-labeled the organ? – MattDMo Jul 8 '13 at 21:06
I don't think so.. because testes sample has high level of protamine expression which is not there in brain. Also, the highest expressed transcript (all RNAs) in brain is Bc1 - a ncRNA known to regulate translation at synapses. – WYSIWYG Jul 9 '13 at 3:53

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