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I'd like to regularly coat some dishes with collagen (Rat Tail, Sigma C7661) for use with PC12 cells. As I only need a small amount each time, I would like to know about any experience in freezing collagen aliquotes to about -20C. The vendors all state that it is not recommended, but the sources have some conflict in interest... Did you find any deterioration in quality? What is your thawing protocol? In what solution do you freeze it and does the concentration matter?

Thanks!

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Have you tried just storing it at +4C? As long as you keep the solution sterile you should be OK. –  MattDMo Jul 30 '13 at 15:57
    
Hi Matt, thanks for your answer. In the end I bought the ready made solution, which has a shelf life of one year. The Sigma support said that the 0.4% solution can be frozen, the 0.01% working solution couldn't. But they also said that freezing did not prolong shelf life. So I'll keep it in the fridge for now. –  malumno Aug 13 '13 at 11:08

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