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I am interested in using the CI and Mnt proteins as well as their respective promoters (pR and Pmnt) within the same synthetic system, but am concerned as to their similarity and potential crosstalk. A quick search of the literature yielded no results and a Multalin did show some significant sequence homology (although Mnt forms a homodimer while CI does not).

CI and Mnt Multalin

pR and Pmnt Multalin

Sequences: CI and pR, Mnt repressor and Mnt promoter

Has it ever been reported that the CI or Mnt repressor exhibit crosstalk on the pR and Pmnt promoters?

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