A protein produced by the B-cells of the immune system which binds to a particular antigen, a foreign substance to the body.
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bind antibodies to study properties
I am interested to experimentally look at the behavior of antibodies. To do so, I need to be able to bind these antibodies somewhere on a sample. does anybody know of a good sample to use where ...
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How does the immune system distinguish own and foreign antibodies?
Therapeutic antibodies, for example Rituximab which recognises CD20 on B lymphoma cells, can cause adverse effects (e.g. http://www.ncbi.nlm.nih.gov/pubmed/19399690)
One reason behind these adverse ...
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What is immunopanning (vs. immunoprecipitation and FACS)?
I had never heard the term before today. From what I can tell, it's using antibodies to purify a cell population of interest. I would appreciate more details, especially in how it differs from ...
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variation in antigen binding site of antibodies
Antibodies or immunoglobulins are proteins made by the immune system in response to alien(!) molecules. Each antibody binds to its specific antigen. This great diversity and specificity is cause of ...
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How to evaluate the efficacy of an antibody for fluorescence microscopy?
I'm trying to evaluate data taken from fluorescence microscopy with antibody staining, and am wondering whether there is any standard way to evaluate the specificity of the antibody for such ...
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Questions regarding ELISA
I have recently peformed an ELISA (Enzyme-linked immunosorbent assay), but I still have some questions, let me first outline what I did:
We had a number (20) of tubes containing fake 'bodily fluids' ...
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How are antibodies designed?
Antibodies have the ability of recognising highly specific peptide sequences and bind it at their antigen-binding site.
This ability is harnessed as a tool in research to purify target structures in ...
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Is there a maximum amount of antibodies your body can keep?
I am wondering if you were theoretically able to get vaccinations or antibodies for any and every diseases and/or illness, would there be a limit to how many you can get and keep in your body at one ...
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Structure of RAP Antibodies (Specifically RAP-5)
[EDIT] - Have just found not one but two papers that address my structure problem. However they concern RAP-1A, so I guess my question is now what is the difference in structure and function of ...
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Recommended applications for commercial antibodies
Commercial suppliers of primary antibodies for given protein targets typically list recommended applications, for which the antibody has presumably been shown to work. I am usually looking to use them ...
