A protein produced by the B-cells of the immune system which binds to a particular antigen, a foreign substance to the body.

learn more… | top users | synonyms

1
vote
1answer
19 views

How to validate the use of an anti-virus monoclonal antibody in IHC by spiking a fresh organ with infected cultured cells?

I have assessed the specificity of a particular monoclonal antibody against a virus by immunofluorescence. I'd like to further validate it by testing it by IHC. Therefore I'd like to infect cells in ...
3
votes
0answers
47 views

How can cells produce antibodies despite error-checking mechanisms in their genomes?

How can immune cells produce so many different kinds of antibodies with different variable regions if there are so many mechanisms inside the cells that try to keep the DNA sequence as constant as ...
2
votes
0answers
55 views

Does antibody staining / immunolabeling block or inhibit protein function?

In dissociated live cells, does staining them with an antibody block the protein's function? I am sorting cells based on their expression of a few marker genes, and culturing them post-sort for ...
0
votes
0answers
31 views

Is it possible to be more tolerant to mosquito bites after being bitten many times

I heard some people (a Swedish colleague told me) in north Scandinavia go naked somewhere where are many mosquitoes and let them bite for a while. Then they claim that they are ready to last the ...
0
votes
0answers
33 views

How can site-directed mutagenesis be used to suppress the production of anti-antibodies?

In a previous post of mine, I asked how to supress the creation of anti-antibodies in vivo. In the answer, it was mentioned that site-directed mutagenesis could be used. Currently, I can't find mcuh ...