The assay-development tag has no wiki summary.
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What would be the best design for spike-and-recovery and linearity-of-dilution validation experiments in one 96-well ELISA plate?
I have searched in the web for a detailed explanation of doing such validation experiment, but unfortunately couldn't find a satisfactory one. I came across the following sources:
Thermo ...
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Primary cilia: what cell types have non-motile cilia that migrate?
My understanding is that there are two broad categories of cilia: motile and non-motile (also called primary.
Examples of the former include sperm flagella and the cilia of epithelial cells that ...
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Providing small molecules to cells on a filter plate
Lets imagine that I have mammalian cells that I've immobilized on a filter. Now I want to keep providing small molecules to these immobilized cells without resolubilizing the cells.
The caveat is ...
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Serological assays not detecting native proteins
Is there anyone out there who has done much work with serological assays? We have antiserum for a manufactured viral protein but no luck so far getting it to detect native protein (unless today's ...
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Ideal low-protein binding membranes
I'm looking through the literature on the topic. It seems like hydrophilic PVDF membranes are ideal for low-protein binding but it also sounds like Regenerated Cellulose is an appropriate ...
