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2
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0answers
42 views

T cell sensitivity and persistence to specific bacterial proteins

Currently, the standard tests for Lyme Disease measure antibody production after exposure to a bacteria, Borrelia burgdorferi. Often, the tests are performed too soon after infection, before ...
3
votes
2answers
48 views

Can ELISA be used to detect a plant enzyme? Creating assay for a new enzyme

If the goal is to generate a rapid assay for an enzyme of plant source what are the typical options? i.e. Could one do something like: Generate an antibody to the enzyme and then use it to create an ...
1
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0answers
34 views

Assays to determine competitive binding versus non-competitive

I'm looking for both simple and complex assays or technologies than can be used to determine if two competing molecules are competitive or noncompetitive. I figure xray crystallography is a clear ...
1
vote
0answers
129 views

How to calculate the LOB, LOD and LOQ of an enzyme assay

I understand how to calculate limit of blank (LOB), limit of detection (LOD), and limit of quantitation (LOQ) in the traditional way i.e., average and SD of raw analytical signal of blanks and low ...
1
vote
1answer
18 views

Colormetric assay for a phenol product?

I am working on an assay for phenol products created by a colony of bacteria. I have searched up a paper that involves gibbs reageant but the protocol is kind of unclear. Does any of you know where I ...
0
votes
0answers
22 views

Determining hyaluronic acid hydrolysis via dye-binding assay

I have a protein that I am reasonably sure hydrolyzes hyaluronic acid, and I'm trying to design an experiment to test that. I was told that a dye binding plate assay where clear zones will indicate ...
1
vote
1answer
44 views

Is galvinoxyl antioxidant assay possible using NMR spectroscopy?

I would like to perform an antioxidant assay using the galvinoxyl protocol. The protocol states that we need EPR spectroscopy, but only NMR spectroscopy is available at my institution. Is there an ...
1
vote
1answer
916 views

Background correction when reading ELISA with TMB substrate

I'm doing some ELISA development, and I'd like some justification/best practices for background correction. I'm using a horseradish-peroxidase (HRP) detection system along with a TMB substrate and an ...
2
votes
1answer
39 views

Is NADPH unstable in UV light?

I am working on an enzyme activity assay using NADPH as a cofactor. The MSDS for NADPH Tetrasodium Salt, tells to store it in a place away from heat and light. Does this include UV light or just ...
1
vote
1answer
145 views

Choosing the best assay for my experiment? [closed]

Which of the following assays can be used to determine whether cell death specifically due to apoptosis has occurred in a given cell sample? Choose all that apply. WST-1 Assay Caspase Colorimetric ...
0
votes
0answers
107 views

What is the probe that absorbs at 450nm in the presence of NADH in this assay?

The colorimetric assays by Biovision and Sigma Aldrich seem to utilise a probe that binds to or reacts with NADH in order to cause absorbance at 450nm which can then be quantified by a ...
7
votes
1answer
93 views

Providing small molecules to cells on a filter plate

Lets imagine that I have mammalian cells that I've immobilized on a filter. Now I want to keep providing small molecules to these immobilized cells without resolubilizing the cells. The caveat is ...
2
votes
1answer
108 views

Pectinase Enzyme Assay

I am working on pectinase enzyme assay. I incubated 900 ul of substrate for 10 minutes in the water bath, followed by adding 2ml of DNSA reagent, then 100ul of enzyme extract added finally i read the ...
6
votes
1answer
381 views

Primary cilia: what cell types have non-motile cilia that migrate?

My understanding is that there are two broad categories of cilia: motile and non-motile (also called primary. Examples of the former include sperm flagella and the cilia of epithelial cells that ...
2
votes
2answers
95 views

Assays during drug discovery

After researching the definition of Assay, I am left with the idea that an assay refers to scientific screening. It could be of chemicals, microbes, etc. I understand that during drug-discovery ...
2
votes
1answer
2k views

What would be the best design for spike-and-recovery and linearity-of-dilution validation experiments in one 96-well ELISA plate?

I have searched in the web for a detailed explanation of doing such validation experiment, but unfortunately couldn't find a satisfactory one. I came across the following sources: Thermo ...
6
votes
1answer
131 views

Ideal low-protein binding membranes

I'm looking through the literature on the topic. It seems like hydrophilic PVDF membranes are ideal for low-protein binding but it also sounds like Regenerated Cellulose is an appropriate ...
5
votes
0answers
53 views

Serological assays not detecting native proteins [closed]

Is there anyone out there who has done much work with serological assays? We have antiserum for a manufactured viral protein but no luck so far getting it to detect native protein (unless today's ...