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6
votes
1answer
139 views

Primary cilia: what cell types have non-motile cilia that migrate?

My understanding is that there are two broad categories of cilia: motile and non-motile (also called primary. Examples of the former include sperm flagella and the cilia of epithelial cells that ...
2
votes
2answers
68 views

Assays during drug discovery

After researching the definition of Assay, I am left with the idea that an assay refers to scientific screening. It could be of chemicals, microbes, etc. I understand that during drug-discovery ...
1
vote
1answer
857 views

What would be the best design for spike-and-recovery and linearity-of-dilution validation experiments in one 96-well ELISA plate?

I have searched in the web for a detailed explanation of doing such validation experiment, but unfortunately couldn't find a satisfactory one. I came across the following sources: Thermo ...
6
votes
1answer
115 views

Ideal low-protein binding membranes

I'm looking through the literature on the topic. It seems like hydrophilic PVDF membranes are ideal for low-protein binding but it also sounds like Regenerated Cellulose is an appropriate ...
5
votes
0answers
43 views

Providing small molecules to cells on a filter plate

Lets imagine that I have mammalian cells that I've immobilized on a filter. Now I want to keep providing small molecules to these immobilized cells without resolubilizing the cells. The caveat is ...
4
votes
0answers
39 views

Serological assays not detecting native proteins

Is there anyone out there who has done much work with serological assays? We have antiserum for a manufactured viral protein but no luck so far getting it to detect native protein (unless today's ...