The tag has no wiki summary.

learn more… | top users | synonyms

1
vote
1answer
57 views

Background correction when reading ELISA with TMB substrate

I'm doing some ELISA development, and I'd like some justification/best practices for background correction. I'm using a horseradish-peroxidase (HRP) detection system along with a TMB substrate and an ...
0
votes
0answers
46 views

What is the probe that absorbs at 450nm in the presence of NADH in this assay?

The colorimetric assays by Biovision and Sigma Aldrich seem to utilise a probe that binds to or reacts with NADH in order to cause absorbance at 450nm which can then be quantified by a ...