A subdivision of microbiology dedicated to the study of bacteria.

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Glucose Oxidase, Condition to be Active?

I most recently was reading/watching information on honeys antibatical and antimicrobial properties. The enzyme Glucose Oxidase came up often as a main antimicrobial, yet it is inactive in honey. ...
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Why does cold kill humans but not bacteria? [closed]

Bacteria can continue like nothing happened after being exposed to low temperatures why doesn't this happen to humans as well? Why can't our metabolic machinery continue as normal?
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How can you develop a bacterial infection when taking antibiotics for another area of the body with a bacterial infection? [closed]

How can you develop a bacterial infection when taking antibiotics for another area? For example, "if someone was prescribed an antibiotic for a bacterial infection of the throat and while taking the ...
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Lysis of bacteria by complement system

The complement system creates pores in cell membrane which leads to influx of lots of water thereby causing lysis of bacterial cell. But what I fail to understand is that if bacteria have cell walls ...
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49 views

Do bacteria grow on pure dry glucose?

I've accidentally touched pure glucose with my bare hands (fingers to be specific), which was intended for cell-culture. I'm worried that bacteria from my skin may start to grow on the glucose and ...
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Serological assays measuring antibody response

Given that an appropriate immune response to a bacteria may be thwarted in an individual, including not producing all of the antibodies which are known to occur in people who have been infected, or ...
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Did the Great Oxygenation Event also cause a mass extinction?

Its usually assumed that the Great Oxidation Event around 2.3 bya caused a great extinction of anaerobic life on earth. But as I was reading Nick Lane's book, ...
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Did Cyanobacteria alone create the oxygen in Earth's atmosphere 3.5 billion years ago?

In other words, is the conveniently close-to-Californian-Universities pools of such representative of accepted theory. With the immediate follow-up question, when did the much more impressive amount ...
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What did cyanobacteria gain from photosynthesis?

As far as I understand, cyanobacteria began performing photosynthesis long before plant cells as we know them arrived on the scene. But cyanobacteria do not seem to use polysaccharides in the same way ...
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54 views

Does drinking alcohol having UTI may help to kill the bacteria?

It's well known that ethyl alcohol kills bacteria. And amount of alcohol in urine may be detected by EtG tests (as long as 3-4 days after intake). Does drinking alcohol in reasonable amount by ...
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38 views

Link between NaCl and sulfide reducing bacteria

Could there be any link between the consumption of NaCl and sulfide-reducing bacteria? In my study on mice, sulfide-reducing bacteria (SRB) are more abundant, in high-salt-fed group compared to the ...
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Why doesn't the rest of the body have something like the “blood-brain” barrier to protect itself from pathogens?

According to Wikipedia: "The blood–brain barrier acts very effectively to protect the brain from most pathogens". This is because the pathogens cannot pass through the tight junctions of the ...
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Why does a semi-set curd not set completely after a round of churning?

I left a pot of milk to turn into curd. While it did look like it had turned to curd since it started coming off the sidewall of the pot, but after having churned it, I realized that it wasn't set ...
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How do I “reinoculate” a petri dish with new bacteria?

Hypothetical question, intended to find a general procedure rather than case-specific recommendations: I have a petri dish full of growth medium with a large population of gram-negative, anaerobic ...
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43 views

Comments on Experimental result regarding Anti-Bacterial soap

After using soaps, we rinse our hands with water. Do bacteria from the water attach themselves to our hands again? If we wipe our hands to cloth after washing our hands, do bacteria from the cloth ...
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Why do gram-positive bacteria cause respiratory diseases more frequently than gram-negative bacteria? [closed]

My theory is that aerobic respiration by gram positive bacteria thrives in ideal conditions of the lungs therefore creates respiratory diseases?
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38 views

Is this bacteria or fungi?

I've been growing bacteria on an agar plate and after putting the dishes in the incubator, this is what I saw. I'm new to bacteria identification, and is this just contamination by fungi or is this ...
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1answer
36 views

Detection of bacteria to measure quality of water

I have been working on a science project were I have built a water filter. To prove that it works I would need to test if there are bacteria in a sample which has been treated vs a sample that hasn't ...
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51 views

What prevent us from synthesizing a cell from “scratch”?

In this recent article "Design and synthesis of a minimal bacterial genome", they created a minimal cell with only 473 genes. However, they didn't synthesize all of the necessary components of the ...
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I don't want to know what bacteria is called but if the bacteria from unborn babies have benifits on health? [duplicate]

This question is not the same as the duplicate. I was told that when babies are born they are born with bacteria in their stomach. When a child takes antibiotics are there unique bacteria destroyed ...
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What kind of organism grows in two-day-old lemon water? [closed]

I had a cup of lemon water on my desk that had been sitting out for 2-3 days. Today I took a sip without even realizing this. I looked at the water and it had something growing in it that looked like ...
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Growth charecteristics of Bacillus and Lactobacillus Species [closed]

Does anybody know weather the media (Hichrome Bacillus Agar) supports both Bacillus and Lactobacillus species, it is supposed to be the sample of the Lactobacillus plantarum spread on Hichrome ...
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Do plant and animal bacterial pathogen differ too much?

I will be involved in a plant disease produced by a bacterial pathogen, Acidovorax citrulli. I've previously worked with animal pathogens such as Haemophilus parasuis. Are there any big differences ...
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Can oral bacteria be targeted by white blood cells?

Today I was told from a dentist that a leukocyte is much larger than a bad bacterium. So a leukocyte cannot remove bad bacteria when they hide in very places like the space between the gums and teeth ...
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Multiplication of bacillus subtilis

I have a supplement for use in horticulture. It is a powder that contains mostly subtilis with smaller amounts of a few other strains. Prior to horticultural application I would like to ...
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Are there bacteria that respire anaerobically in aerobic conditions?

There are facultative aerobic bacteria that switch to aerobic respiration in an aerobic state, but are there any organisms that would still perform anerobic respiration even when shifted to aerobic ...
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When streaking microbes on a plate to test growth after uv exposure, does how I streak make a difference?

I've been working on what I think might be a special strain of E. coli, I've been streaking it on LB agar plate and exposing it to varied times of UVC via the germicidal hood. I'm wondering if how I ...
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How can I make sure my UV-purificator kills bacteria?

I'm going to show a UV-purificator for water on science fair. How can I easily check if purificator kills most of the bacteria? I need a quick and possibly easy method.
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Reasons for variable quality virus production

I am making MSCV retrovirus, using DH5α cells to amplify a plasmid of interest, qiagen's midiprep kit to purify out the plasmid, then 293ft cells to produce the retrovirus, and 3t3 cells to test MOI. ...
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Effects of antibiotics on bacterial growth [closed]

I will do an experiment on effects of antibiotics on antibacterial growth and I will use three antibiotics: ampicilin, chloramphenicol and streptomycin. To make a hypothesis I must have a guess on ...
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Can bacteria, viruses, and cancer cells be destroyed by resonance?

Radiotherapy has been used to treat cancer. Can the resonances by coordinated electromagnetic waves (and/or other forms of waves), of various frequencies, amplitudes and pulse rates, directed from ...
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How to promote denitrifying microbe activity

I'm an amateur fresh-water aquarist looking at the problem of nitrate reduction (into largely-inert nitrogen gas) in a small-scale aquarium environment. The process of turning the byproducts of fish ...
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57 views

Why doesn't one develop immunity to bacterial STIs?

Why doesn't one develop immunity to STIs such as Chlamydia/Gonorrhea and Syphilis even after the first exposure and treatment with antibiotics?
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Bacteria surviving a β-lactam antibiotic

What changes can occur in the cell wall of a bacteria for it to survive a β-lactam antibiotic? I think that because a bacteria possesses peptidoglycan in its cell wall, they are β-lactam sensitive, ...
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What is a catalytic domain?

I have to answer a few questions from an article I'm reading, and I am just really confused on defining "catalytic domain." I have to relate it to the antibiotic Teixobactin. Is catalytic domain the ...
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How is a bacterial strain defined?

When a species of bacteria is referred to by its strain, are they a clone of single founder or is a certain amount variation allowed?
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How long can you effectively store a glycerol stock at -20 degrees Celsius?

I know that glycerol stocks are typically kept in a -80 °C freezer, however there are some people who do not have access to such equipment. How long would you be able to keep a glycerol stock at ...
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How do microbes develop resistance to anitmicrobial peptides?

I would like to better understand how bacteria use the "strategy" of alternations to lipid A and membrane proteins in order to resist antimicrobial peptides of the immune system? It is my ...
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Pink growth (aka pink slime) in bathtub or shower

I was wondering if anybody had studied or was knowledgeable about the pink growth that people often find around water fixtures in their homes, especially bath tubs and showers? My understanding is ...
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Are Betaproteobacteria more 'simple' than Gammaproteobacteria?

More generally, are all proteobacteria named in order of complexity (Alpha, Beta, Gamma, Delta, Epsilon)? If so, would it be right to say that Betaproteobacteria can survive in lower nutrition / more ...
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how to calculate dilution factor and CFU

I'm very confused I did a bacterial experiment where i added 5 grams to 70ml of distilled water that contains 10g of fresh meat. I left it in a sampler container over 24 hours then i cultured ...
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Feasibility of “smart effective microorganisms”

This might be a stupid question as I have little to no knowledge of biology, but I would really like to find out if it would be feasible to make such a system as a way of preventing infections. I ...
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What is the advantage of using plant-derived antibacterials rather than bacteria-derived antibacterials?

So obviously we have a big problem with antibiotic resistance. Most of our antibiotics originate from bacteria themselves (or are synthetic variations on scaffolds which originate from bacteria). I ...
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Advocate for the pop-culture idea of withholding vaccination [closed]

While it is widely accepted that vaccination is preferable to not vaccinating, would anyone like to give a shot at providing evidence in favor of not vaccinating? Anything goes.
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Is it worth to experiment with pro-biotics at home?

Let's assume that Joe has done some investigation on Internet and wants to see, if pro-biotics would help him. Now he goes to amazon.com and buys a pro-biotic product with excellent average rating. ...
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Can an argument be made that humans are 90% bacterial?

On the blog, All about Scientist in Microword: Microbiology, I read the post We are 90% bacteria, actually, which says that humans are 90% bacterial cells. If this is the case, then why don't we ...
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Is 100% antibacterial resistance possible?

The quest to develop antibacterial and antibiotic substances has often been described as an arms-race, as bacterial life develops resistance, requiring us to develop novel agents which can effectively ...
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Microbiology- Pseudomonas aeruginosa, BSAC data

I tested Pseudomonas aeruginosaagainst various antibiotics, using Stokes and Kirby-Bauer. When I compared my results with BSAC data it was completely different (understandable) but why for a lot of ...
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What is the difference between a bacterial artificial chromosome (BAC) and a plasmid?

Is it just that a BAC is generally larger and artificially constructed? Or are there any other differences?
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Why do bacteria need messenger RNA?

Why do bacteria need mRNA? Isn't the DNA free floating without nuclear membrane so why doesn't the tRNA read the code directly off of the split DNA strands? (ofcourse after helicase splits them). ...