A subdivision of microbiology dedicated to the study of bacteria.

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Is it safe to refill a PET water bottle? [on hold]

At work, I drink quite a lot of water by refilling a PET water bottle such as this one and it could happen that I use the same bottle for at least two weeks. I know that the plastic might dissolve ...
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How can E. coli proliferate so rapidly?

The E. coli has a genome with approximately 5×106 bp. The main DNA polymerase involved in its chromosome duplication (DNA pol III , the one with highest processivity) can polymerize ~103 nucleotides ...
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is Acinetobacter spp produce biofilm [on hold]

Is Acinetobacter spp. produce biofilm ? And what the basic properties of this bacteria like biochemical test?And some literature about Acinetobacter.
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Survival of streptococcus after my scarlet fever

I'm currently sick because of scarlet fever but I got treated with antibiotics. I know that after 1.5 day using antibiotics the streptococci I release at home are dangerous anymore. But my question ...
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234 views

Are bacteria necessary for an individual's life, or, how long could a person live without bacteria? [closed]

Bacteria are essential to life in that they are responsible for breakdown of organic substances, etc. but are bacteria necessary for an individual's life? In other words, how long would a human ...
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Why are plasmid genes not already incorporated into bacterial chromosomes if necessary for stressful situations?

If plasmids are important for bacteria to express specific genes under stressful conditions, why are these genes not already incorporated into their chromosome to begin with? What is the evolutionary ...
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Can I use yogurt to prevent the growth of black mold?

I have a mold problem in my bathroom. There is a narrow groove between the top of my tub and the wall it is attached to where a black gunk repeatedly grows. I can shoot the material out with a spray ...
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What are the requirements for a stable carbon cycle in sealed “bottle gardens”`?

The most famous example of a sealed bottle garden is David Latimer's bottle with a Spiderworth plant, pictured below, which has been sealed for 40 years. My own attempt at a bottle garden is failing ...
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What microscope/magnification would I need to observe P. Acnes bacteria?

I am currently attempting to grow a culture of P. Acnes bacteria. Right now, my only hope in identifying colonies of the bacteria in the culture is to use a black light to find colonies that glow ...
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How to characterise a protein family in a putative genome island?

We have sequenced the genome of 200 bacterial strains belonging to the same species, a swine bacterial pathogen. In a previous work, it was observed that a protein family of adhesins is present in ...
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eradicating h.pylori naturally

This question is not to discuss whether substances with anti-h.pylori agents works or not, or the pros and cons of such but from a medical perspective. Assuming that one manage to control, reduce, or ...
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Does Pseudomonas aeruginosa have any common name at all?

Pseudomonas aeruginosa is the fifth most written about¹ binomial name of all species. I'm having trouble coming to terms with the possibility that such a well researched and studied organism has never ...
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How much of the weight of yoghurt is due to bacteria?

I recently started making all kinds of bacteria-processed foods by myself (such as yoghurt, but also Sauerteig and so on), and I found myself wondering how much of the weight of these products is ...
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Do bacteria with multiple flagella move faster than bacteria with a single flagella?

Do bacteria with multiple flagella move faster than bacteria with a single flagella? Assuming the flagella are at the same length.
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What does the gene name “lexA” stand for?

It is an important gene expressed in E. coli that represses the SOS response and also the expression of lambda lytic phase genes. UV light and damage to DNA is responsible for its breakdown and hence ...
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72 views

Production of processed Insulin in bacteria

Genetically-engineered bacteria are used to produce insulin in industry, but as far as I know, the bacteria can produce only proinsulin. Why is that? What happens in the human body in order to make ...
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Medium for Pseudomonas?

What is a good minimal medium for Pseudomonas bacteria? Do I need different media for different Pseudomonas species?
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Hot water and bacteria

I know that it is common to say, "use hot water when washing your hands" or when you've got a cut, "wash your hands with warm water," etc. I was wondering, why is this the case? Since bacteria grow in ...
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Can different strains of Rhizobium share an infection thread or symbiosome?

Rhizobium infection can be triggered at root hairs of legumes, creating infection threads. Can these infections threads be colonized by more than one type of Rhizobium (e.g. Fixing and non-fixing)? ...
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How to identify genes required for biofilm formation

This is just a homework question: Q: Let’s assume that there is an unknown set of E. coli genes that are required for biofilm formation. Describe a genetic experiment you could perform to try to ...
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Nature of plasmids [closed]

What are the plasmids composed of?? Genomic DNA or non-genomic DNA ? I think, the answer should be "genomic DNA"...but am not too sure...
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Are all single-celled organisms Bacteria?

I read that "Bacteria are one-celled organisms that can multiply by division", are all one-celled organisms bacteria or are there any more narrow definitions?
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58 views

How often does bacterial transformation happen?

I have been reading: M. Dröge, A. Pühler, W. Selbitschka, "Horizontal gene transfer among bacteria in terrestrial and aquatic habitats as assessed by microcosm and field studies", Biol. Fertil. ...
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Effect of flask position in shaking incubator affects growth kinetics

During some cultivation experiments for my bachelor thesis I noticed that in liquid culture my bacteria (E. coli) grew differently, according to the position in the shaking incubator. Because the ...
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Are added yogurt cultures harmful to the gut?

I have read somewhere that the live culture added to yogurt acting as "healthy" gut bacteria are actually synthetic and harmful. Any truth to this? If so, what are some alternatives to getting good ...
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Can Hepatitis be caused by Bacteria?

There are many information about viral hepatitis. But it is interesting for me, can bacteria cause the hepatitis too?
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65 views

Will an 11W UVC lamp kill bacteria?

If I shone a UVC lamp (11W) on something (say for example a plastic phone case) for an hour, would that kill off a noticeable amount of bacteria? That is, if I were to go over the case with a cotton ...
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Is there any way the food industry can benefit from biofilms?

I realize all of the disadvantages, but I am wondering if the food industry can actually benefit from the formation of biofilms.
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Does every wave length of UV kill bacteria?

Will every wave length of UV work for killing bacteria? So will UV A, UV B and UV C all work? Or will only the shortest of the three, UV C, work? Also, will the average UV lamp help against bacterial ...
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Can two Hfr strains of E. coli conjugate?

Genetics textbooks (and some internet searching) yield abundant examples of Hfr strains conjugating with F- cells, but these sources are surprisingly silent regarding the results of an Hfr ...
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Why does botulinum toxin seem to be more dangerous to humans than to other mammals?

Various mammals seem to get away with eating parts of carcasses that we would prefer to not even touch, and that we assume will make us sick. Because of that, I assume botulinum toxin is more ...
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How does isopropyl alcohol disinfect less in higher concentration?

Isopropyl alcohol can be used as disinfectant. For increasing concentration of it in water, the effect as disinfectant increases, and then decreases again. Typical concentrations for use as ...
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Can diseases be transmitted wirelessly? [closed]

According to the recent research about Electromagnetic Signals from Bacterial DNA (A. Widom, J. Swain, Y. N. Srivastava, S. Sivasubramanian) it seems there is a possibility of a "wireless" version of ...
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Do bacteria die of old age?

I know that the cells of mammals at least stop dividing when they are old, and then die a programmed cell death. Then other cells have to replace them. But in a bacterial colony, each cell ...
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Why is it that bacteria and fungi cannot develop resistance against essential oils?

I wonder why bacteria and fungi cannot develop resistance and mutate agains essential oils? For example, some fungi get killed by oregano oil. Being not a biologist, I hope my question is not so ...
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Is there a specific word for bacterial death?

What do you call it when a bacterium dies? Cellular death is apoptosis, necrosis, and bacterial is ...? I don't simply want to write in a paper that it - well dies!
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Can viruses that normally infect eukaryotic cells also infect bacteria?

Can standard viruses infect bacteria? I'm not speaking of bacteriophage but typical RNA and DNA virus such as influenza and Ebola.
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Does E. coli survive at any pH level?

Does E. coli survive at any pH level? If I was to incubate it in agar of different pH, would it still form a bacterial lawn as it's called?
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51 views

How long does it take for commercial fertilizers to break down? [closed]

Sometimes we buy carrots that are abit bitter, it's the taste of fertilzer. Once i put a tomato in the fridge, and when i ate it, it was bitter like shweppes, and i figured that was fertilizer also. ...
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Why isn't Bubonic Plague as virulent as it once was?

Although I am admittedly not anywhere near an expert on any topic, I still have a hard time understanding how Black Death and Bubonic plague are one in the same. Since plague still exists, with news ...
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What is the mechanism of oxygen uptake in E. coli?

How does E. coli uptake oxygen? Most of the literature I found is concerned with response to oxygen level supplied in the medium, as opposed to how much is actually transported inside. Can they shut ...
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What is the fastest growing bacterium?

E. coli has a generation time of about 20 min, and a single CFU will generate a colony a few millimeters in diameter after 16 hours of incubation. Is this the upper limit? How much faster can ...
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51 views

What are the dimensions in angstrom of bacteria? [closed]

I'm going to take a picture of a bacteria but I am unsure how large it is. What are the dimensions in angstrom of bacteria?
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Can a bacteriophage be used to treat bacterial diseases?

Some bacteriophages reproduce using the lytic cycle which ends with the destruction of the host bacterial cell. I was wondering if theoretically this could be used theraputically to treat bacterial ...
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Differential Scanning Calorimetry for bacterial membranes

I would like to study the freezing and melting of bacterial membranes and would like to use Differential Scanning Calorimetry (DSC) to obtain the glass transition temperature of the membrane. However, ...
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2k views

Calculation of the bacterial growth rate from a spectrophotomer growth curve

Typically the microbial growth in liquid cultures is monitored by turbidity. Data is obtained with a spectrophotometer to measure optical density at 600nm. The slope of the bacterial kinetic curve in ...
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Are efflux pumps mutations or are they originally present in N. gonorrhoea?

I'll start by saying that I am no biologist. I am a math major trying to create a mathematical model for gonorrhea strain competition. My assignment is to create a model similar to a model that was ...
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Could the growth rates of Vibrio vulnificus be affected by an oil spill?

I live on the Gulf Coast, and Vibrio vulnificus has recently made the news due to an increase in the number of reported infections. Naturally, it has people around here worried. I live in an area that ...
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What makes certain obligate anaerobes viable in fermentation starter cultures?

If Propionibacterium are obligate anaerobes (to wit, poisoned by oxygen), what makes 'Dairy' or classical propionibacteria (e.g. P. shermanii, P. jensenii, P. acidicpropionici, et al) viable in ...
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How does sucrose protect bacterial cells in lysozyme solutions?

I have a microbiology question. When we put bacterial cells in sucrose solution with concentration higher than 0.5M we observe plasmolysis - the cytoplasmic membrane detaches from the cell wall due ...