A subdivision of microbiology dedicated to the study of bacteria.

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Can viruses infect bacteria?

Can standard viruses infect bacteria? I'm not speaking of bacteriophage but typical RNA and DNA virus such as influenza and Ebola.
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Does E. coli survive at any pH level?

Does E. coli survive at any pH level? If I was to incubate it in agar of different pH, would it still form a bacterial lawn as it's called?
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Is there a natural defense against an increase in E.Coli level in natural water systems?

I was recently on an Oceanology coliform collection vessel in the Long Island Sound and I found out that human waste contains E.Coli and when not properly treated the bacteria can run off or ...
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19 views

How long does it take for commercial fertilizers to break down? [on hold]

Sometimes we buy carrots that are abit bitter, it's the taste of fertilzer. Once i put a tomato in the fridge, and when i ate it, it was bitter like shweppes, and i figured that was fertilizer also. ...
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Why isn't Bubonic Plague as virulent as it once was?

Although I am admittedly not anywhere near an expert on any topic, I still have a hard time understanding how Black Death and Bubonic plague are one in the same. Since plague still exists, with news ...
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What is the mechanism of oxygen uptake in E. coli?

How does E. coli uptake oxygen? Most of the literature I found is concerned with response to oxygen level supplied in the medium, as opposed to how much is actually transported inside. Can they shut ...
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What is the fastest growing bacterium?

E. coli has a generation time of about 20 min, and a single CFU will generate a colony a few millimeters in diameter after 16 hours of incubation. Is this the upper limit? How much faster can ...
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43 views

What are the dimensions in angstrom of bacteria? [closed]

I'm going to take a picture of a bacteria but I am unsure how large it is. What are the dimensions in angstrom of bacteria?
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Can a bacteriophage be used to treat bacterial diseases?

Some bacteriophages reproduce using the lytic cycle which ends with the destruction of the host bacterial cell. I was wondering if theoretically this could be used theraputically to treat bacterial ...
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Differential Scanning Calorimetry for bacterial membranes

I would like to study the freezing and melting of bacterial membranes and would like to use DSC to obtain the glass transition temperature of the membrane. However, I am unsure where to find such ...
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Calculation of the bacterial growth rate from a spectrophotomer growth curve

Typically the microbial growth in liquid cultures is monitored by turbidity. Data is obtained with a spectrophotometer to measure optical density at 600nm. The slope of the bacterial kinetic curve in ...
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Are efflux pumps mutations or are they originally present in N. gonorrhoea?

I'll start by saying that I am no biologist. I am a math major trying to create a mathematical model for gonorrhea strain competition. My assignment is to create a model similar to a model that was ...
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Could the growth rates of Vibrio vulnificus be affected by an oil spill?

I live on the Gulf Coast, and Vibrio vulnificus has recently made the news due to an increase in the number of reported infections. Naturally, it has people around here worried. I live in an area that ...
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What makes certain obligate anaerobes viable in fermentation starter cultures?

If Propionibacterium are obligate anaerobes (to wit, poisoned by oxygen), what makes 'Dairy' or classical propionibacteria (e.g. P. shermanii, P. jensenii, P. acidicpropionici, et al) viable in ...
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how to measure sugar concentration in growth medium

I am growing up bacteria in a flask and was wondering how do I track the changes of sugar concentration from the growth medium in the flask. Thanks for the help
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How does sucrose protect bacterial cells in lysozyme solutions?

I have a microbiology question. When we put bacterial cells in sucrose solution with concentration higher than 0.5M we observe plasmolysis - the cytoplasmic membrane detaches from the cell wall due ...
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To what extent is it possible to understand if a bacteria can produce a protein? (in silico only!)

I have to answer a question about a sub-task of my thesis: Can the bacteria X produce the protein Y? I searched of course Google and BLAST. There is no data supporting that this specific bacteria ...
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86 views

How does heat shock transformation work?

What exactly happens when competent cells like DH5ɑ are heatshocked with DNA present? How does the DNA get inside the cells? Specifically, why are all the steps necessary? What if you heatshock right ...
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32 views

What causes the odor when a container is sealed with moisture inside?

I'm not sure if this is a biology or chemistry question since I don't know the cause of it (though I think it is bacteria or fungal related). I'm just wondering what creates that awful odor when a ...
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Transportation of bacteria

I would like to take an apparatus containing bacteria to another country (Bolivia) from the U.S.. How do I determine whether I am allowed to do this? The bacteria are: ...
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Blood consumption

Is consumption of blood more "dangerous" compared to meat? There was a news-article about unnatural chemicals found in the blood of mothers. This reminded me about a question I have pondered upon ...
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Restriction endonucleases are found in?

Quoting from : Scientific American July 1975 The Manipulation of genes by Stanley Cohen : Restriction endonucleases (and modification methylases) are widespread in microorganisms; genes for ...
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How Sex Evolved ? and when?

As I know evolution comes bit by bit mutation by mutation How sex evolved which requires a major change in at least two individuals one to become male and one to become female ? When that happened ...
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What is the film that covers the tongue?

What is the film that covers the tongue in the mornings, even after brushing the teeth and tongue the night before and why does it have color variations? Do the different colors mean anything?
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How is bacterial plasma membrane made?

Eukaryotes have ER which manufactures plasma membrane of cells. How is prokaryotic plasma membrane made ? What is the pathway and which enzymes are involved ?
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Property of E.coli harboring F element

From Genetics by Ursula Goodenough E.coli harboring an F element are endowed with a number of phenotypic traits : 1.They are sensitive to infection by ssRNA phages and certain ssDNA phages. ...
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Functioning of EDTA

I know that EDTA chelates metal ions. It weakens bacterial cell wall and inactivates the DNases. What is the reason why it can do so ? I guess it can inactivate DNases by altering the ...
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Buy bacteria for experiment?

Where can I buy paracoccus denitrificans? I need it for an experiment. I cannot find online where I can purchase this.
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Functioning of BCG vaccine

I read (from Nature Volume507, S4–S7 (06 March 2014) : For reasons that are poorly understood, BCG protects only infants; it is ineffective in older children and adults. Its efficacy also ...
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How quickly is antibiotic resistance lost?

I would imagine the bacterial genome is highly conserved and limited in its space, but maybe I am wrong. If you were to take a strain of antibiotic resistant bacteria and kept them isolated, but fed ...
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College Bio Research: Bacteria found on computer keyboards. [closed]

I am a college students brainstorming ideas on different topics that may be appropriate for on-campus research. What I came up with and I would like to give it a try is analyze the different types of ...
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261 views

How do a viruses or bacteria survive outside the body long enough to spread?

Say I cough on my table, then someone else touches it and picks up something I've got... how is it that these things can live outside the body, how long can they manage it, and how long is generally ...
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Bacterial Conjugation/Horizontal Gene Transfer — how does the plasmid exchange work?

So according to a PPT I'm reading, bacterial conjugation works by the two bacteria joining pili and exchanging plasmids. So how exactly do the plasmids get across the gap? If I understand this ...
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Do lysogenic viruses only infect bacteria?

In the video sent by my teacher on viruses, the example he used for lysogenic viruses was a bacteriophage infecting a bacteria. When he was describing how the genetic material was incorporated into ...
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Quorum Sensing in Vibrio cholerae

This is a figure summarising the quorum sensing mechanism in Vibrio cholerae. In this video by Bonnie Bassler, she explains how quorum sensing can be targeted to control infections. At 15:09 she ...
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How can promoter binding sites be determined?

I have been trying to find out which sigma factor is responsible for the transcription of RNA polymerase subunits $\alpha$ (rpoA) and $\beta ^{\prime}$ (rpoC) in Bacillus subtilis. I would expect it ...
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Does antibacterial soap work chemically?

Normal soap requires scrubbing, does anti-bacterial soap require scrubbing too? Since it (I assume) works chemically, can I produce the same cleansing effect without lathering with soap and using ...
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Prepare Mueller-Hinton agar at home

I'm doing a self science project, it's easy if i have a lot of money to buy a Mueller-Hinton agar bottle from Ebay for about 110$ (include shipping cost). The main problem is it is very expensive for ...
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I don't wash my hands and never get sick. Any theories? [closed]

I have only been sick a few times in my life(I'm 21 and probably 3-4 times) and even when I am sick I don't feel that bad and I am over it within a day or two. My family has always given me slack for ...
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Correlating Ki values of pesticides to bacterial growth

Four pesticides P1 to P4 are reversible inhibitors of an enzyme E that is essential for the growth of a bacterium B. Their Ki values are given in the table below. Each of these four pesticides ...
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Correlating B-lactamase expression to absorbance

When penicillin-resistant bacteria are grown in liquid culture media, B -lactamase is secreted into the medium. The supernatant of such a medium can be assayed for B- lactamase activity. Culture ...
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Replication of DNA in E. coli: what are DARS and RIDA?

I understand what is DnaA, its role in replication and the fact that it's only active when binding ATP. I don't understand what are DARS and RIDA and how they control the amount of DnaA-ATP:DnaA-ADP
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What is the maximum amount of different bacteria in the drinking water in Europe?

I am looking for a statistical amounts which are allowed. Some students say it is 0 for all bacteria, which I think is false. I found this USA source. I found there Total Coliforms (including fecal ...
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Why do peas change colour?

I am doing an experiment on preservation of peas. The first notable change to the peas over a period of one week is that they have changed colour from bright green to a dull colour... Why is this ...
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Why bacteria produce light?

After seeing this video I am curious to know why the free living bacteria produce light. What advantage will they have ? Or is it just an "unintentional" result of one of their pathways ?
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Is it possible for bacteria to acquire family-wide antibiotic resistance?

If there is bacteria, lets say Enterococci that is sensitive to: Amoxycillin-Clavulinic acid Ampicillin-Sulbactam Ofloxacin Ciprofloxacin Is it possible to ...
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Can antibiotic resistant bacteria compete with normal one in an antibiotic free environment?

The question is based on an intuition that antibiotic resistance can't come along. This mutation will probably make bacteria less tenacious. Is there any research how AR bacteria compete with normal ...
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Gene transfer between Gram positive and Gram negative bacteria?

How does transfer of genetic material occur between Gram positive and Gram negative bacteria?
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Speciation and Phylogeny of Lactobacillus

The lactobacillus, also called Döderlein's bacillus is a genus of facultative aerobic bacteria. There are several species such as Lactobacillus acidophillus and Lactobacillus reuteri. I have several ...
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List of all reported bacteria

I would like to know were to download a table file with all reported bacteria and its corresponding PHYLUM, CLASS, ORDER and FAMILY. Basically, its the same table than this one from GOLD genomes but ...