A subdivision of microbiology dedicated to the study of bacteria.

learn more… | top users | synonyms (1)

2
votes
0answers
17 views

How does sucrose protect bacterial cells in lysozyme solutions?

I have a microbiology question. When we put bacterial cells in sucrose solution with concentration higher than 0.5M we observe hemolysis - the cytoplasmic membrane detaches from the cell wall due to ...
0
votes
1answer
38 views

To what extent is it possible to understand if a bacteria can produce a protein? (in silico only!)

I have to answer a question about a sub-task of my thesis: Can the bacteria X produce the protein Y? I searched of course Google and BLAST. There is no data supporting that this specific bacteria ...
5
votes
1answer
70 views

How does heat shock transformation work?

What exactly happens when competent cells like DH5ɑ are heatshocked with DNA present? How does the DNA get inside the cells? Specifically, why are all the steps necessary? What if you heatshock right ...
0
votes
0answers
29 views

What causes the odor when a container is sealed with moisture inside?

I'm not sure if this is a biology or chemistry question since I don't know the cause of it (though I think it is bacteria or fungal related). I'm just wondering what creates that awful odor when a ...
2
votes
0answers
53 views

Transportation of bacteria

I would like to take an apparatus containing bacteria to another country (Bolivia) from the U.S.. How do I determine whether I am allowed to do this? The bacteria are: ...
1
vote
0answers
40 views

Blood consumption

Is consumption of blood more "dangerous" compared to meat? There was a news-article about unnatural chemicals found in the blood of mothers. This reminded me about a question I have pondered upon ...
3
votes
3answers
98 views

Restriction endonucleases are found in?

Quoting from : Scientific American July 1975 The Manipulation of genes by Stanley Cohen : Restriction endonucleases (and modification methylases) are widespread in microorganisms; genes for ...
6
votes
2answers
199 views

How Sex Evolved ? and when?

As I know evolution comes bit by bit mutation by mutation How sex evolved which requires a major change in at least two individuals one to become male and one to become female ? When that happened ...
3
votes
1answer
46 views

What is the film that covers the tongue?

What is the film that covers the tongue in the mornings, even after brushing the teeth and tongue the night before and why does it have color variations? Do the different colors mean anything?
0
votes
1answer
19 views

How is bacterial plasma membrane made?

Eukaryotes have ER which manufactures plasma membrane of cells. How is prokaryotic plasma membrane made ? What is the pathway and which enzymes are involved ?
0
votes
1answer
24 views

Property of E.coli harboring F element

From Genetics by Ursula Goodenough E.coli harboring an F element are endowed with a number of phenotypic traits : 1.They are sensitive to infection by ssRNA phages and certain ssDNA phages. ...
1
vote
2answers
43 views

Functioning of EDTA

I know that EDTA chelates metal ions. It weakens bacterial cell wall and inactivates the DNases. What is the reason why it can do so ? I guess it can inactivate DNases by altering the ...
1
vote
1answer
49 views

Buy bacteria for experiment?

Where can I buy paracoccus denitrificans? I need it for an experiment. I cannot find online where I can purchase this.
3
votes
1answer
25 views

Functioning of BCG vaccine

I read (from Nature Volume507, S4–S7 (06 March 2014) : For reasons that are poorly understood, BCG protects only infants; it is ineffective in older children and adults. Its efficacy also ...
2
votes
3answers
83 views

How quickly is antibiotic resistance lost?

I would imagine the bacterial genome is highly conserved and limited in its space, but maybe I am wrong. If you were to take a strain of antibiotic resistant bacteria and kept them isolated, but fed ...
2
votes
0answers
37 views

College Bio Research: Bacteria found on computer keyboards. [closed]

I am a college students brainstorming ideas on different topics that may be appropriate for on-campus research. What I came up with and I would like to give it a try is analyze the different types of ...
2
votes
3answers
141 views

How do a viruses or bacteria survive outside the body long enough to spread?

Say I cough on my table, then someone else touches it and picks up something I've got... how is it that these things can live outside the body, how long can they manage it, and how long is generally ...
1
vote
1answer
63 views

Bacterial Conjugation/Horizontal Gene Transfer — how does the plasmid exchange work?

So according to a PPT I'm reading, bacterial conjugation works by the two bacteria joining pili and exchanging plasmids. So how exactly do the plasmids get across the gap? If I understand this ...
1
vote
1answer
42 views

Do lysogenic viruses only infect bacteria?

In the video sent by my teacher on viruses, the example he used for lysogenic viruses was a bacteriophage infecting a bacteria. When he was describing how the genetic material was incorporated into ...
2
votes
1answer
43 views

Quorum Sensing in Vibrio cholerae

This is a figure summarising the quorum sensing mechanism in Vibrio cholerae. In this video by Bonnie Bassler, she explains how quorum sensing can be targeted to control infections. At 15:09 she ...
6
votes
1answer
99 views

How can promoter binding sites be determined?

I have been trying to find out which sigma factor is responsible for the transcription of RNA polymerase subunits $\alpha$ (rpoA) and $\beta ^{\prime}$ (rpoC) in Bacillus subtilis. I would expect it ...
1
vote
2answers
64 views

Does antibacterial soap work chemically?

Normal soap requires scrubbing, does anti-bacterial soap require scrubbing too? Since it (I assume) works chemically, can I produce the same cleansing effect without lathering with soap and using ...
0
votes
0answers
43 views

Prepare Mueller-Hinton agar at home

I'm doing a self science project, it's easy if i have a lot of money to buy a Mueller-Hinton agar bottle from Ebay for about 110$ (include shipping cost). The main problem is it is very expensive for ...
-1
votes
1answer
161 views

I don't wash my hands and never get sick. Any theories? [closed]

I have only been sick a few times in my life(I'm 21 and probably 3-4 times) and even when I am sick I don't feel that bad and I am over it within a day or two. My family has always given me slack for ...
1
vote
1answer
26 views

Correlating Ki values of pesticides to bacterial growth

Four pesticides P1 to P4 are reversible inhibitors of an enzyme E that is essential for the growth of a bacterium B. Their Ki values are given in the table below. Each of these four pesticides ...
1
vote
1answer
27 views

Correlating B-lactamase expression to absorbance

When penicillin-resistant bacteria are grown in liquid culture media, B -lactamase is secreted into the medium. The supernatant of such a medium can be assayed for B- lactamase activity. Culture ...
0
votes
0answers
44 views

Replication of DNA in E. coli: what are DARS and RIDA?

I understand what is DnaA, its role in replication and the fact that it's only active when binding ATP. I don't understand what are DARS and RIDA and how they control the amount of DnaA-ATP:DnaA-ADP
0
votes
1answer
34 views

What is the maximum amount of different bacteria in the drinking water in Europe?

I am looking for a statistical amounts which are allowed. Some students say it is 0 for all bacteria, which I think is false. I found this USA source. I found there Total Coliforms (including fecal ...
1
vote
1answer
47 views

Why do peas change colour?

I am doing an experiment on preservation of peas. The first notable change to the peas over a period of one week is that they have changed colour from bright green to a dull colour... Why is this ...
1
vote
1answer
53 views

Why bacteria produce light?

After seeing this video I am curious to know why the free living bacteria produce light. What advantage will they have ? Or is it just an "unintentional" result of one of their pathways ?
0
votes
0answers
22 views

Is it possible for bacteria to acquire family-wide antibiotic resistance?

If there is bacteria, lets say Enterococci that is sensitive to: Amoxycillin-Clavulinic acid Ampicillin-Sulbactam Ofloxacin Ciprofloxacin Is it possible to ...
1
vote
3answers
77 views

Can antibiotic resistant bacteria compete with normal one in an antibiotic free environment?

The question is based on an intuition that antibiotic resistance can't come along. This mutation will probably make bacteria less tenacious. Is there any research how AR bacteria compete with normal ...
-1
votes
1answer
96 views

Gene transfer between Gram positive and Gram negative bacteria?

How does transfer of genetic material occur between Gram positive and Gram negative bacteria?
2
votes
0answers
38 views

Speciation and Phylogeny of Lactobacillus

The lactobacillus, also called Döderlein's bacillus is a genus of facultative aerobic bacteria. There are several species such as Lactobacillus acidophillus and Lactobacillus reuteri. I have several ...
1
vote
1answer
142 views

List of all reported bacteria

I would like to know were to download a table file with all reported bacteria and its corresponding PHYLUM, CLASS, ORDER and FAMILY. Basically, its the same table than this one from GOLD genomes but ...
3
votes
1answer
68 views

When might an inhibitor of bacterial transformation be useful?

I am part of a project elucidating some structures that are required for bacterial transformation. We have the opportunity to screen inhibitors of the system to stop it from functioning. I am not a ...
2
votes
1answer
50 views

Why is Thermus aquaticus and Taq Pol stable?

What is the molecular basis of the stability of the bacterium Thermus aquaticus and the enzyme Taq Pol it produces ? I have tried googling this but did not get a good answer.
1
vote
2answers
187 views

Blast a sequence against multiple databases

I would like to BLASTP a list of protein sequences in fasta format against multiple protein databases. Since I'm only interested in the first hit of each database, it is possible that BLAST result ...
0
votes
1answer
21 views

How to get conservation of vaccine candidates between hundreds of bacterial strains?

We have identified vaccine candidates via an ex-vivo RNA-seq approach. Next step would be to perform conservation of these candidates (about 20) between multiple bacterial strains (about 200). I would ...
4
votes
1answer
94 views

Does conjugation support gene selection?

I have already posted this on chat but haven't got any response. A recent question on group selection stimulated me to ask this here. QUESTIONS: Why should bacteria conjugate? If we consider that a ...
2
votes
1answer
99 views

Mesosome - does it exist?

This wikipedia page says that mesosomes are artifacts produced by chemical fixing techniques. Most of my textbooks still show a mesosome and describes it as having functions like - respiration, ...
1
vote
3answers
83 views

Assay for Beta-galactosidase activity in single cell microscopy

I'd like to be able to measure the activity of $\beta$-galactosidase in living cells with simple optical (maybe fluorescence) microscopy. Ideally I'd like to do a minimum of genetic engineering, and ...
1
vote
0answers
23 views

Improving throughput of CFU plating?

In a separate question I've described my general experimental setup where I need to measure the number of live cells in a growing bacterial culture in a fairly rapid and high-throughput manner. In ...
6
votes
1answer
212 views

Alternatives to CFU plating for measuring number of viable cells?

I am hoping to measure growth rates of a bacterial culture in several growth conditions. I am concerned that these growth conditions may cause cell death, which would lead to a decreased ...
2
votes
0answers
147 views

Image Processing Suite for bacterial microscopy: Schnitzcells or MicrobeTracker?

I am looking to start doing some work tracking the size and growth of individual bacterial cells in the microscope. In order to analyze the images I need software that can segment the cells, ...
2
votes
2answers
163 views

How much natural genetic variation is there between bacteria of the same species?

There are lots of questions about how and why there is genetic variation between organisms of the same species, but I haven't been able to find a numerical value for the expected amount of genetic ...
1
vote
1answer
134 views

Why does beta-2-Thienylalanine inhibit bacterial growth?

I'm trying to obtain a better understanding of the Guthrie Test, which checks whether people have a disease called PKU. The Guthrie test uses bacteria to check for Phenylalanine (the amino acid) in ...
6
votes
1answer
157 views

Whole genome amino acid composition tool?

I'm interested in a statistical tool to get bacterial codon usage at genomic level. Ideally, the tool should be flexible to analyse hundreds of bacterial genomes. I've looked in MeSH terms database ...
4
votes
4answers
98 views

How to measure bacterial content

I´m using water container placed on a radiator to humidify room air. Someone might argue that it is a perfect nest for bacteria. I have to put copper coins into the water to kill the bacteria. I ...
4
votes
1answer
536 views

Why do different bacteria have different shapes?

Why do different bacteria have different shapes? Is it only related to their function?