A subdivision of microbiology dedicated to the study of bacteria.

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How effective are restriction enzymes in protecting bacteria?

Bacteria use restriction enzymes to cut DNA of bacteriophages. Virus mutates really fast. Won't a point mutation in restriction site render the restriction enzymes of the bacteria useless ? So how ...
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Do bacteria have any mechanism to protect themselves from RNA bacteriophage?

Bacteria can cut up DNA of DNA bacteriophages by using restriction enzymes. Of what I know about restriction enzymes, I think they don't cut RNA. So, do bacteria have any mechanism through which they ...
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Which strains of C. diphteria are causing Diphteria toxin?

I got this question: Diphteria toxin is produced only by those strains of C. diphtheria that have which of the following characteristics? Encapsulated glucose fermenters lysogenic for b-prophage ...
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Why restriction enzymes cut (usually) at palindromic sequences?

Restriction enzymes usually cut only at palindromic sequences. Is there any specific reason for that ? Is there any advantage for bacteria if it cuts up virus at this type of sequences ?
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What is the advantage of restriction enzymes cutting only at specific sites?

Bacteriophages have sequences which often do not have specific sites for restriction enzymes of bacteria to cut at and so can attack the bacteria. Wouldn't it be better if bacteria had something ...
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How do plasmids protect themselves against restriction enzymes?

How do plasmids protect themselves from restriction enzymes released by bacteria (i.e., against bacteriophages)?
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Why do plasmids replicate on their own?

I know how plasmids can replicate independently of the main genome and know that they confer various properties to the bacteria and are useful in conjugation. My question is - what is the advantage ...
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Can viruses protect themselves against restriction enzymes?

Restriction enzymes cut the DNA of bacteriophages. Have bacteriophages evolved any mechanism to protect themselves from it ?
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Is iodised salt harmful to the lactofermentation process?

I was discussing sauerkraut recipes with someone on IRC a minute ago, and they warned me not to use iodised salt in the process. My first (and so far, the only successful) batch is one I made with ...
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Metawatt database setup

I am currently trying to setup Metawatt on my server but am running into trouble as described in my email to the author (who has an auto reply that he will not answer before September). So any help ...
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372 views

How to obtain bacteria samples at home?

As the original question went from hold to closed, I thought I would write up a more appropriate question. How should one go about getting bacterial samples to look at under a microscope at home? ...
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Do toxin producing strains of E. coli constantly produce toxin or only under certain conditions?

Background E. coli is prevalent everywhere but only some strains produce toxins harmful to humans, such as E. coli O157:H7. Questions 1) Would anyone know if these strains are always producing ...
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What are the functions of magnetic bacteria?

I'm trying to understand why are bacteria "equipped" with magnetosomes and why/what for do they need such organelles.
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Aside from cockroaches and other insects, which species (of all kinds) are 'immune' to ionizing radiation?

I have just been reading this excellent question and answer about cockroaches ability to withstand ionizing radiation. My question is an extension of that question: aside from insects, which other ...
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363 views

Possibility and feasibility of producing designed antibodies with bacteria

As stated here it is possible to produce fragments of antibodies in bacteria and harvest them (from the medium, I guess, but I don't have access to the full article). As it is possible to design ...
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213 views

What is a good Microbiology atlas for Bacteriology as online version?

I am looking for a online atlas for Bacteriology, the cost does not matter. I have been searching SpringerLink, and Amazon but no good online version found. What is a good atlas for Bacteriology as a ...
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683 views

What are the differences between G+ and G- bacteria?

The distinction between Gram-positive and Gram-negative bacteria is based upon the Gram staining method, that reflects the bacterial wall physical properties. However, this classification involves ...
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Is there a practical upper limit to amount of nucleotides or genes in a transformed plasmid?

I'm currently working on a synthetic biology project which involves working with lots of different parts. I would ultimately like to integrate these genes by transforming a single plasmid. I've heard ...
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What effect do oligosaccharides, like those found in legumes, have on the composition of intestinal flora in humans and if so how so?

I know that intestinal flora metabolising oligosaccharides, like those found in legumes, is the cause for the well known fact that legumes cause flatulence, but does an oligosaccharide-rich intestine ...
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How can I save bacillus strains on filter paper without an -80 degree freezer?

I want to save my bacillus strains but I don't have access to a -80 degree freezer. What are possible alternatives?