A subdivision of microbiology dedicated to the study of bacteria.

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Why is Thermus aquaticus and Taq Pol stable?

What is the molecular basis of the stability of the bacterium Thermus aquaticus and the enzyme Taq Pol it produces ? I have tried googling this but did not get a good answer.
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204 views

Blast a sequence against multiple databases

I would like to BLASTP a list of protein sequences in fasta format against multiple protein databases. Since I'm only interested in the first hit of each database, it is possible that BLAST result ...
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1answer
21 views

How to get conservation of vaccine candidates between hundreds of bacterial strains?

We have identified vaccine candidates via an ex-vivo RNA-seq approach. Next step would be to perform conservation of these candidates (about 20) between multiple bacterial strains (about 200). I would ...
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1answer
95 views

Does conjugation support gene selection?

I have already posted this on chat but haven't got any response. A recent question on group selection stimulated me to ask this here. QUESTIONS: Why should bacteria conjugate? If we consider that a ...
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1answer
110 views

Mesosome - does it exist?

This wikipedia page says that mesosomes are artifacts produced by chemical fixing techniques. Most of my textbooks still show a mesosome and describes it as having functions like - respiration, ...
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3answers
92 views

Assay for Beta-galactosidase activity in single cell microscopy

I'd like to be able to measure the activity of $\beta$-galactosidase in living cells with simple optical (maybe fluorescence) microscopy. Ideally I'd like to do a minimum of genetic engineering, and ...
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0answers
24 views

Improving throughput of CFU plating?

In a separate question I've described my general experimental setup where I need to measure the number of live cells in a growing bacterial culture in a fairly rapid and high-throughput manner. In ...
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1answer
236 views

Alternatives to CFU plating for measuring number of viable cells?

I am hoping to measure growth rates of a bacterial culture in several growth conditions. I am concerned that these growth conditions may cause cell death, which would lead to a decreased ...
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0answers
162 views

Image Processing Suite for bacterial microscopy: Schnitzcells or MicrobeTracker?

I am looking to start doing some work tracking the size and growth of individual bacterial cells in the microscope. In order to analyze the images I need software that can segment the cells, ...
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2answers
167 views

How much natural genetic variation is there between bacteria of the same species?

There are lots of questions about how and why there is genetic variation between organisms of the same species, but I haven't been able to find a numerical value for the expected amount of genetic ...
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1answer
149 views

Why does beta-2-Thienylalanine inhibit bacterial growth?

I'm trying to obtain a better understanding of the Guthrie Test, which checks whether people have a disease called PKU. The Guthrie test uses bacteria to check for Phenylalanine (the amino acid) in ...
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1answer
162 views

Whole genome amino acid composition tool?

I'm interested in a statistical tool to get bacterial codon usage at genomic level. Ideally, the tool should be flexible to analyse hundreds of bacterial genomes. I've looked in MeSH terms database ...
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4answers
110 views

How to measure bacterial content

I´m using water container placed on a radiator to humidify room air. Someone might argue that it is a perfect nest for bacteria. I have to put copper coins into the water to kill the bacteria. I ...
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2answers
601 views

Why do different bacteria have different shapes?

Why do different bacteria have different shapes? Is it only related to their function?
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208 views

What are artifacts in microbiology?

What kind of objects can be qualified as artifacts? How do we distinguish such objects?
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1answer
72 views

Aerobe or facultative anaerobe organism that metabolizes acetate?

I'm assuming bacteria, but will take any suggestions. Organism must survive primarily on the acetate (plus trace elements), but I can give/take electrons, if necessary. Not interested in strict ...
3
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1answer
234 views

How do I measure bacterial growth in agar dishes (either by cell mass or by cell count)?

I am doing an experiment in which I am growing S. mutans in agar dishes, and I am not sure how I would measure the growth of the S. mutans. I am also not sure if I would do this by measuring cell mass ...
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0answers
59 views

What are some readily available, easy to test, bacteria?

I want to do a science fair experiment about how bacteria interact with 5-carbon vs. 6-carbon sugars, and I want a bacteria that is easy to test and is readily available. Any ideas?
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0answers
33 views

Are there any gluten-eating bacteria that are readily available?

Is there some yeast or bacterium that will eat gluten? I was hoping for some kind of yeast, but I can't find one. Where could I find one?
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1answer
124 views

Expression of plasmid genes

Are plasmid genes always expressed? If so, then isn't a bacterium wasting it's resources in expressing genes (like antibiotic resistance) which are not required in "normal" conditions? If not, then ...
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1answer
60 views

Extract bacteria from compost?

I'm working on a project where I need to find certain cellulolytic bacteria. I was looking at this list : ...
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1answer
61 views

Are there any bacteria that can receive ultrasound signals?

I'm looking for an example of bacteria that could receive ultrasound (at any ultrasound frequency) signal and possibly perform some (re)action afterwards.
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How long can C. Tetani survive in soil?

Clostridium tetani (C. tetani) is a bacterium commonly found in soil and is excreted in the faeces of many animals (both mammals and birds) and serves, by means of the exotoxin, tetanospasmin, causes ...