A subdivision of microbiology dedicated to the study of bacteria.

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Why isn't the RNA in bacteria always split up and replicating?

Isn't helicase always free floating in bacterial cells, and the DNA without a nuclear membrane and uncoiled and freefloating and so why doesn't the helicase keep breaking the double helix of DNA? ...
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57 views

How does the immune system recognize pathogens?

There are useful and pathogenic bacteria in our body. How does the immune system differentiate between them?
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How much genomic variation one usually find within a given bacterial species?

If I find the exact starting position (say 1152471) of the coding sequence of a given gene in the genome of a bacterium, is the genome of the bacterium in general stable enough so that I can expect to ...
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percentage of animal diseases caused by bacteria?

I know this is a long shot, but what is the approximate percentage out of all the animal diseases that are caused by bacteria? One of the Q&A websites answer it as 90, but is there any conclusive ...
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56 views

Why are bacterial cultures necessary?

I notice that in a lot of bacteria testing procedures the sample has to be cultured. You place the sample in agar or another growth medium and wait for the bacteria to proliferate before examining ...
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49 views

Using evolution of bacteria against themselves

We know that mutations happen regularly in bacteria and also that one bacteria might get the mutation and become stronger than the others and thus survive, causing antibiotic resistance as well. Can ...
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53 views

Examining milk for presence of harmful bacteria

I am not experienced in diagnostic bacteriology and am interested in understanding more about it in the context of analyzing food purity. For example, imagine I have a supply of raw milk which might ...
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335 views

Can bacteria release free DNA into their environment?

Natural transformation AKA natural competence involves the uptake of DNA into a competent bacterium (for horizontal gene transfer or as a food source). My question is about where this extracellular ...
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232 views

Does soap kill human cells?

I see many products, particularly hand soap and cleaning products, that claim to kill 99.9% or more of bacteria. This makes me wonder, if the chemicals are potent enough to break down bacterial cell ...
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119 views

If all bacteria in human gut are dead, can they be there again?

If someone likes to eat chocolate, she may have gut bacteria which like chocolate according to some articles that I have read. ...
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66 views

Favored Conditions of Bacterial Growth

I have read that bacteria "thrive" in warm places. Naturally, I am very interested in why this is the case. Humans for instance thrive also in relatively warm conditions if it's too cold or too warm ...
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70 views

Bacteria resistance to natural antibiotics? [duplicate]

It is a known fact that bacteria develop immunity to antibiotics. Why then did not all bacteria develop immunity to natural antibiotics like penicillin in the fungi? It is for sure that fungi did not ...
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69 views

Why a particular species of bacteria give rise to particular type of colony? [closed]

Bacterial colony varies in form, elevation, margin, opacity, chromogenesis etc. What gives definite character to a colony and what is the source of all the diversity? Is the reason similar to that of ...
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How do I test if microbes have survived after dehydration?

I have a solution containing various bacteria and fungi. My aim is to place solution on filter paper, and wait until it dries. I then wish to test if the organisms have survived, either on the dried ...
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77 views

Do adult humans exchange beneficial bacteria? If so, how?

Recently there has been increasing interest and research into symbiotic bacteria present in humans and human gut. I'm aware of two new discoveries: humans are surrounded by a unique cloud of ...
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45 views

Are there antibiotics for all bacteria somewhere in the earth? [closed]

When I read about Penicillin, I found that it came from a green mold to protect itself. If all bacteria and/or molds in competition to survive, can we believe that there is an antibiotic for each ...
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28 views

Why would a bacterial population show initial growth when it is in unfavorable growth conditions?

This figure shows the anaerobe E. faecalis grown in aerobic conditions, E. coli grown in restricted conditions that are not specified. Why do they show an increase in abundance initially? The black ...
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130 views

Two different sized colonies from the same species of bacteria. What does it mean? [closed]

I have got two different sized colonies in a plate of Salmonella Paratyphi, identified by biochemical methods. What does two different sized colonies mean? What kind of question can I investigate in ...
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68 views

How do you interpret this microbiology/ bacteriology research figure?

This is a figure from a research article that I am to do a senior presentation for. It is showing bacterial replication of an enteric bacteria population. The researchers hypothesize that the ...
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145 views

Can general soap kill bacteria?

I have read that general soap can kill bacteria by opening holes in the bacterial membrane. http://questions.sci-toys.com/node/90 However, I found some articles as well saying that it cannot. ...
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70 views

What if there are no bacteria such as Streptococcus mutans

If someone removed all bacteria such Streptococcus mutans in her mouth by starving long time, or by some chemical method, would she never have such bacteria? In my thought, because S.mutans only ...
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Why don't bacteria eat food leftovers?

I have thrown a dirty spoon after eating some pasta into one of my desk drawers (doesn't do me much honor). It stayed there for around a year. My house is warm and I think there is enough humidity for ...
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Which Lactobacillales (Lactic acid bacteria — LAB) strains are capable of degrading starch?

I've been researching bacteria strains but am having trouble finding amylolytic LAB strains with amylolytic and lactic acid producing character. The only species I've found, that are capable of ...
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206 views

Does a microwave oven disinfect food?

Imagine I am preparing food, just about to put it into a microwave oven, and some of it falls on the floor. Assuming it got some bacteria or other organisms (viruses?) on it, will the microwave ...
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64 views

How can E. coli affect C. elegans expression?

Plasmids can be transferred to E. coli. These transformed E. coli can be fed to C. elegans to silence its gene expression by RNAi. How can E.coli release RNAi to C. elegans? Even if we assume E. ...
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575 views

How heavy are all foreign microorganisms in and on the human body?

I define "foreign microorganism" as a microorganism which is not produced by the human body (not antibodies or leukocytes) including bacteria, viruses, fungi, biofilm aggregates or small lifeforms ...
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Enrichment of slow growing microbes: Large Test Tubes as an alternative?

Well, i would like to enrich bacterial and archaeal ammonia oxidisers from soil samples using Minimal Salt media. They are slow growing microbes. I would like to enrich them with the aim to produce ...
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50 views

Blood testing for chlamydia

I am working with the bacterium Chlamydia trachomatis. The main test for this species is the naat test, which is based on amplification. But I would like to know how the bacteria affects the blood ...
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How many diseases can be linked to disruption in the microbiome of a human?

I was listing to the radio and heard recent research found a link between children and higher cases of asthma when certain bacteria are missing from the microbiome. How many other diseases can be ...
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Can microorganisms (e.g. bacteria) be damaged or killed by 253,7 nm light?

The question arose in a hospital, where I saw these flyers advertising lamps that emit such light. It was claimed that they can clean the air of microorganisms up to an arbitrary degree. Does anyone ...
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63 views

Are aquatic filter feeders effective at cleaning water to a safe level?

I've been raising Daphnia magna cultures over the last few months as feedstock for freshwater aquarium fish. From my reading, Daphnia feeds on bacteria and single-celled algae, as well as other small ...
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Need clarification on a sentence regarding vesicle-mediated translocation

I've chanced upon a passage that is not entirely clear to me: In addition to conjugation, transformation and transduction, other less well recognised mechanisms of DNA uptake occur in nature, ...
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70 views

What does NaOH do for the plating of Bacillus subtilis?

A book called Molecular Biological Methods for Bacillus called for plating Bacillus subtilis cells on LB-agar plates with the following components: A general-purpose medium containing per litre: ...
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What is the effect of antibiotic chloramphenicol on bacterial size?

When low amounts of antibiotics like Chloramphenicol is added in a bacterial culture, the steady-state growth rate decreases but what happens to the steady-state size? Can anybody please refer me to ...
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What is the mechanism by which bactericides kill bacteria? [closed]

"Bactericides kill bacteria". What is the meaning of "killing" in this context, and how exactly is this "killing" happening chemically?
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Decomposition Rate of A Body In A Wet Environment [closed]

I have a photo of a decomposing dog which is in a water logged environment. My peers and I are debating how long that body has been decomposing. They are saying it is at least a year old. I however ...
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Unsure why Synechococcus moves at angle in cuvette

I'd really appreciate your help. I'm working with Synechococcus. In my experiment, I have Synechococcus in a solution of milli-Q water. I illuminated it with a focused white light through a pinhole ...
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158 views

What are the evidence that all life today descended from a common ancestor (LUCA), and which organisms (if any) challenge the concept?

If I understand correctly, the concept of the LUCA (last universal common ancestor) is based on the hypothesis that archaea and bacteria share common ancestry. In the realm of mathematics, the same ...
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232 views

Approximately how long do bacteria live for? [closed]

I would like to know how many days or years do the bacteria live approximately.
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What bacteria do unborn babies contain?

This Scientific American article states that "[human] infestation [by bacteria] begins at birth". This would suggest that unborn babies are free from any bacteria. However, if the mother catches a ...
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Secretion in Gram negative bacteria

Among the 6 secretion systems in bacteria, can these 6 ways be sorted out in the order of how harmful it is to the human host? Like say type 3 is highly virulent so that comes first, but I don't know ...
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Requirements for soil sample transportation over long distance

I need to collected soil sample from agricultural area far away from my lab. It would required about a week for the sample to arrive to lab. I need the soil sample for genomic DNA isolation ...
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Is it possible to merge the genomes of photosynthesizing and electron-transporting bacteria to make an electric cell?

I had an idea of making electric current from bacteria. There are some bacteria that can photosynthesize, and some others are capable of transmitting electrons through each other. Is it possible that ...
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What are the most important factors influencing a person’s gut microbes?

You are your bacteria! The probiotics and the antibiotics... There has been on going discussions about how our gut bacteria is important for a healthy lifestyle. Figure 1: Schematic diagram ...
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What concentration of table salt is needed to kill bacteria in sweaty gym clothes?

My gym clothes retain an unpleasant musk even after washing them with warm water and detergent. I've experimented with soaking my gym clothes in salty water before washing them. My hypothesis is that ...
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186 views

Why do some protocols require prewarming a liquid medium before inoculating?

For example, in this protocol for E. coli competent cell preparation, it says: Plate 10 uL E. coli BL21(DE3) cells on a LB-agar plate; incubate overnight (12 hours). Prepare 500 mL SOB medium ...
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What bacteria shed harvestable energy from root zone organic matter?

I recently heard of a successful effort that harvests energy from soil at plants' bases due to apparently bacterial breakdown of wastes from the plants: Via photosynthesis a plant produces organic ...
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Why pick just a single bacterial transformed colony

So after bacteria have been transformed to perhaps grow up a plasmid of interest, why pick only a single bacterial colony from a selective plate for further expansion? I understand that this is to ...
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395 views

Do I need to serially dilute E. coli cultures for optical density measures?

I am expected to track cell growth by measuring the incubating culture's cell concentration every 30 minutes or so. So my questions are: Why do I need to do serial dilution (assuming that I do)? Why ...
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Risks in bacterial phage therapy

I just finished reading J. Craig Venter's book Life at the Speed of Light: From the Double Helix to the Dawn of Digital Life. The book is a little over a year old now, and Venter has an optimistic ...