A subdivision of microbiology dedicated to the study of bacteria.

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How many diseases can be linked to disruption in the microbiome of a human?

I was listing to the radio and heard recent research found a link between children and higher cases of asthma when certain bacteria are missing from the microbiome. How many other diseases can be ...
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67 views

Horizontal Gene Transfer

I understand the different ways bacteria can undergo horizontal gene transfer (transformation, transduction (phages), conjugation (plasmids)). Is there an experimental method to tell how a specific ...
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145 views

Does Pseudomonas aeruginosa have any common name at all?

Pseudomonas aeruginosa is the fifth most written about¹ binomial name of all species. I'm having trouble coming to terms with the possibility that such a well researched and studied organism has never ...
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136 views

Will an 11W UVC lamp kill bacteria?

If I shone a UVC lamp (11W) on something (say for example a plastic phone case) for an hour, would that kill off a noticeable amount of bacteria? That is, if I were to go over the case with a cotton ...
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87 views

Aerobe or facultative anaerobe organism that metabolizes acetate?

I'm assuming bacteria, but will take any suggestions. Organism must survive primarily on the acetate (plus trace elements), but I can give/take electrons, if necessary. Not interested in strict ...
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96 views

Have there been any experiments that duplicate chromosome copies from 23*2 to 23*3 or 23*4?

Deinococcus radiodurans is an amazing bacterium with a fantastic survival rate. It can survive to high doses of radiation, in a complete vacuum and in hydrochloric acid. How does this bacterium ...
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What is the advantage of using plant-derived antibacterials rather than bacteria-derived antibacterials?

So obviously we have a big problem with antibiotic resistance. Most of our antibiotics originate from bacteria themselves (or are synthetic variations on scaffolds which originate from bacteria). I ...
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42 views

How much genomic variation one usually find within a given bacterial species?

If I find the exact starting position (say 1152471) of the coding sequence of a given gene in the genome of a bacterium, is the genome of the bacterium in general stable enough so that I can expect to ...
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92 views

Two different sized colonies from the same species of bacteria. What does it mean? [closed]

I have got two different sized colonies in a plate of Salmonella Paratyphi, identified by biochemical methods. What does two different sized colonies mean? What kind of question can I investigate in ...
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Can two Hfr strains of E. coli conjugate?

Genetics textbooks (and some internet searching) yield abundant examples of Hfr strains conjugating with F- cells, but these sources are surprisingly silent regarding the results of an Hfr ...
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197 views

Can diseases be transmitted wirelessly? [closed]

According to the recent research about Electromagnetic Signals from Bacterial DNA (A. Widom, J. Swain, Y. N. Srivastava, S. Sivasubramanian) it seems there is a possibility of a "wireless" version of ...
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130 views

Transportation of bacteria

I would like to take an apparatus containing bacteria to another country (Bolivia) from the U.S.. How do I determine whether I am allowed to do this? The bacteria are: ...
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330 views

Image Processing Suite for bacterial microscopy: Schnitzcells or MicrobeTracker?

I am looking to start doing some work tracking the size and growth of individual bacterial cells in the microscope. In order to analyze the images I need software that can segment the cells, ...
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217 views

Functioning of EDTA

I know that EDTA chelates metal ions. It weakens bacterial cell wall and inactivates the DNases. What is the reason why it can do so ? I guess it can inactivate DNases by altering the ...
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233 views

List of all reported bacteria

I would like to know were to download a table file with all reported bacteria and its corresponding PHYLUM, CLASS, ORDER and FAMILY. Basically, its the same table than this one from GOLD genomes but ...
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32 views

Why are bacterial cultures necessary?

I notice that in a lot of bacteria testing procedures the sample has to be cultured. You place the sample in agar or another growth medium and wait for the bacteria to proliferate before examining ...
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71 views

What are the dimensions in angstrom of bacteria? [closed]

I'm going to take a picture of a bacteria but I am unsure how large it is. What are the dimensions in angstrom of bacteria?
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Buy bacteria for experiment?

Where can I buy paracoccus denitrificans? I need it for an experiment. I cannot find online where I can purchase this.
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What are the differences between G+ and G- bacteria?

The distinction between Gram-positive and Gram-negative bacteria is based upon the Gram staining method, that reflects the bacterial wall physical properties. However, this classification involves ...
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41 views

What is a catalytic domain?

I have to answer a few questions from an article I'm reading, and I am just really confused on defining "catalytic domain." I have to relate it to the antibiotic Teixobactin. Is catalytic domain the ...
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47 views

Using evolution of bacteria against themselves

We know that mutations happen regularly in bacteria and also that one bacteria might get the mutation and become stronger than the others and thus survive, causing antibiotic resistance as well. Can ...
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Can microorganisms (e.g. bacteria) be damaged or killed by 253,7 nm light?

The question arose in a hospital, where I saw these flyers advertising lamps that emit such light. It was claimed that they can clean the air of microorganisms up to an arbitrary degree. Does anyone ...
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712 views

What concentration of table salt is needed to kill bacteria in sweaty gym clothes?

My gym clothes retain an unpleasant musk even after washing them with warm water and detergent. I've experimented with soaking my gym clothes in salty water before washing them. My hypothesis is that ...
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81 views

Why is it that bacteria and fungi cannot develop resistance against essential oils?

I wonder why bacteria and fungi cannot develop resistance and mutate agains essential oils? For example, some fungi get killed by oregano oil. Being not a biologist, I hope my question is not so ...
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326 views

Why does beta-2-Thienylalanine inhibit bacterial growth?

I'm trying to obtain a better understanding of the Guthrie Test, which checks whether people have a disease called PKU. The Guthrie test uses bacteria to check for Phenylalanine (the amino acid) in ...
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How effective are restriction enzymes in protecting bacteria?

Bacteria use restriction enzymes to cut DNA of bacteriophages. Virus mutates really fast. Won't a point mutation in restriction site render the restriction enzymes of the bacteria useless ? So how ...
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Why don't bacteria eat food leftovers?

I have thrown a dirty spoon after eating some pasta into one of my desk drawers (doesn't do me much honor). It stayed there for around a year. My house is warm and I think there is enough humidity for ...
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145 views

What are the evidence that all life today descended from a common ancestor (LUCA), and which organisms (if any) challenge the concept?

If I understand correctly, the concept of the LUCA (last universal common ancestor) is based on the hypothesis that archaea and bacteria share common ancestry. In the realm of mathematics, the same ...
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Secretion in Gram negative bacteria

Among the 6 secretion systems in bacteria, can these 6 ways be sorted out in the order of how harmful it is to the human host? Like say type 3 is highly virulent so that comes first, but I don't know ...
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79 views

To what extent is it possible to understand if a bacteria can produce a protein? (in silico only!)

I have to answer a question about a sub-task of my thesis: Can the bacteria X produce the protein Y? I searched of course Google and BLAST. There is no data supporting that this specific bacteria ...
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32 views

Property of E.coli harboring F element

From Genetics by Ursula Goodenough E.coli harboring an F element are endowed with a number of phenotypic traits : 1.They are sensitive to infection by ssRNA phages and certain ssDNA phages. ...
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124 views

Why do peas change colour?

I am doing an experiment on preservation of peas. The first notable change to the peas over a period of one week is that they have changed colour from bright green to a dull colour... Why is this ...
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263 views

Expression of plasmid genes

Are plasmid genes always expressed? If so, then isn't a bacterium wasting it's resources in expressing genes (like antibiotic resistance) which are not required in "normal" conditions? If not, then ...
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85 views

How do plasmids protect themselves against restriction enzymes?

How do plasmids protect themselves from restriction enzymes released by bacteria (i.e., against bacteriophages)?
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Can viruses protect themselves against restriction enzymes?

Restriction enzymes cut the DNA of bacteriophages. Have bacteriophages evolved any mechanism to protect themselves from it ?
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Do toxin producing strains of E. coli constantly produce toxin or only under certain conditions?

Background E. coli is prevalent everywhere but only some strains produce toxins harmful to humans, such as E. coli O157:H7. Questions 1) Would anyone know if these strains are always producing ...
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How is a bacterial strain defined?

When a species of bacteria is referred to by its strain, are they a clone of single founder or is a certain amount variation allowed?
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Why isn't the RNA in bacteria always split up and replicating?

Isn't helicase always free floating in bacterial cells, and the DNA without a nuclear membrane and uncoiled and freefloating and so why doesn't the helicase keep breaking the double helix of DNA? ...
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Are there antibiotics for all bacteria somewhere in the earth? [closed]

When I read about Penicillin, I found that it came from a green mold to protect itself. If all bacteria and/or molds in competition to survive, can we believe that there is an antibiotic for each ...
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Why would a bacterial population show initial growth when it is in unfavorable growth conditions?

This figure shows the anaerobe E. faecalis grown in aerobic conditions, E. coli grown in restricted conditions that are not specified. Why do they show an increase in abundance initially? The black ...
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Is it possible to merge the genomes of photosynthesizing and electron-transporting bacteria to make an electric cell?

I had an idea of making electric current from bacteria. There are some bacteria that can photosynthesize, and some others are capable of transmitting electrons through each other. Is it possible that ...
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75 views

Risks in bacterial phage therapy

I just finished reading J. Craig Venter's book Life at the Speed of Light: From the Double Helix to the Dawn of Digital Life. The book is a little over a year old now, and Venter has an optimistic ...
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292 views

Does E. coli survive at any pH level?

Does E. coli survive at any pH level? If I was to incubate it in agar of different pH, would it still form a bacterial lawn as it's called?
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What is the fastest growing bacterium?

E. coli has a generation time of about 20 min, and a single CFU will generate a colony a few millimeters in diameter after 16 hours of incubation. Is this the upper limit? How much faster can ...
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Are efflux pumps mutations or are they originally present in N. gonorrhoea?

I'll start by saying that I am no biologist. I am a math major trying to create a mathematical model for gonorrhea strain competition. My assignment is to create a model similar to a model that was ...
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304 views

Bacterial Conjugation/Horizontal Gene Transfer — how does the plasmid exchange work?

So according to a PPT I'm reading, bacterial conjugation works by the two bacteria joining pili and exchanging plasmids. So how exactly do the plasmids get across the gap? If I understand this ...
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Correlating Ki values of pesticides to bacterial growth

Four pesticides P1 to P4 are reversible inhibitors of an enzyme E that is essential for the growth of a bacterium B. Their Ki values are given in the table below. Each of these four pesticides ...
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Why bacteria produce light?

After seeing this video I am curious to know why the free living bacteria produce light. What advantage will they have ? Or is it just an "unintentional" result of one of their pathways ?
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402 views

Blast a sequence against multiple databases

I would like to BLASTP a list of protein sequences in fasta format against multiple protein databases. Since I'm only interested in the first hit of each database, it is possible that BLAST result ...
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260 views

Assay for Beta-galactosidase activity in single cell microscopy

I'd like to be able to measure the activity of $\beta$-galactosidase in living cells with simple optical (maybe fluorescence) microscopy. Ideally I'd like to do a minimum of genetic engineering, and ...