A subdivision of microbiology dedicated to the study of bacteria.

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Secretion in Gram negative bacteria

Among the 6 secretion systems in bacteria, can these 6 ways be sorted out in the order of how harmful it is to the human host? Like say type 3 is highly virulent so that comes first, but I don't know ...
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Can Hepatitis be caused by Bacteria?

There are many information about viral hepatitis. But it is interesting for me, can bacteria cause the hepatitis too?
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To what extent is it possible to understand if a bacteria can produce a protein? (in silico only!)

I have to answer a question about a sub-task of my thesis: Can the bacteria X produce the protein Y? I searched of course Google and BLAST. There is no data supporting that this specific bacteria ...
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Property of E.coli harboring F element

From Genetics by Ursula Goodenough E.coli harboring an F element are endowed with a number of phenotypic traits : 1.They are sensitive to infection by ssRNA phages and certain ssDNA phages. ...
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92 views

Why do peas change colour?

I am doing an experiment on preservation of peas. The first notable change to the peas over a period of one week is that they have changed colour from bright green to a dull colour... Why is this ...
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214 views

Expression of plasmid genes

Are plasmid genes always expressed? If so, then isn't a bacterium wasting it's resources in expressing genes (like antibiotic resistance) which are not required in "normal" conditions? If not, then ...
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How do plasmids protect themselves against restriction enzymes?

How do plasmids protect themselves from restriction enzymes released by bacteria (i.e., against bacteriophages)?
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Can viruses protect themselves against restriction enzymes?

Restriction enzymes cut the DNA of bacteriophages. Have bacteriophages evolved any mechanism to protect themselves from it ?
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Do toxin producing strains of E. coli constantly produce toxin or only under certain conditions?

Background E. coli is prevalent everywhere but only some strains produce toxins harmful to humans, such as E. coli O157:H7. Questions 1) Would anyone know if these strains are always producing ...
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Is it possible to merge the genomes of photosynthesizing and electron-transporting bacteria to make an electric cell?

I had an idea of making electric current from bacteria. There are some bacteria that can photosynthesize, and some others are capable of transmitting electrons through each other. Is it possible that ...
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Risks in bacterial phage therapy

I just finished reading J. Craig Venter's book Life at the Speed of Light: From the Double Helix to the Dawn of Digital Life. The book is a little over a year old now, and Venter has an optimistic ...
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215 views

What is the fastest growing bacterium?

E. coli has a generation time of about 20 min, and a single CFU will generate a colony a few millimeters in diameter after 16 hours of incubation. Is this the upper limit? How much faster can ...
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Are efflux pumps mutations or are they originally present in N. gonorrhoea?

I'll start by saying that I am no biologist. I am a math major trying to create a mathematical model for gonorrhea strain competition. My assignment is to create a model similar to a model that was ...
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195 views

Bacterial Conjugation/Horizontal Gene Transfer — how does the plasmid exchange work?

So according to a PPT I'm reading, bacterial conjugation works by the two bacteria joining pili and exchanging plasmids. So how exactly do the plasmids get across the gap? If I understand this ...
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Correlating Ki values of pesticides to bacterial growth

Four pesticides P1 to P4 are reversible inhibitors of an enzyme E that is essential for the growth of a bacterium B. Their Ki values are given in the table below. Each of these four pesticides ...
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73 views

Why bacteria produce light?

After seeing this video I am curious to know why the free living bacteria produce light. What advantage will they have ? Or is it just an "unintentional" result of one of their pathways ?
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Blast a sequence against multiple databases

I would like to BLASTP a list of protein sequences in fasta format against multiple protein databases. Since I'm only interested in the first hit of each database, it is possible that BLAST result ...
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218 views

Assay for Beta-galactosidase activity in single cell microscopy

I'd like to be able to measure the activity of $\beta$-galactosidase in living cells with simple optical (maybe fluorescence) microscopy. Ideally I'd like to do a minimum of genetic engineering, and ...
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Are there any bacteria that can receive ultrasound signals?

I'm looking for an example of bacteria that could receive ultrasound (at any ultrasound frequency) signal and possibly perform some (re)action afterwards.
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Is iodised salt harmful to the lactofermentation process?

I was discussing sauerkraut recipes with someone on IRC a minute ago, and they warned me not to use iodised salt in the process. My first (and so far, the only successful) batch is one I made with ...
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404 views

What is a good Microbiology atlas for Bacteriology as online version?

I am looking for a online atlas for Bacteriology, the cost does not matter. I have been searching SpringerLink, and Amazon but no good online version found. What is a good atlas for Bacteriology as a ...
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Requirements for soil sample transportation over long distance

I need to collected soil sample from agricultural area far away from my lab. It would required about a week for the sample to arrive to lab. I need the soil sample for genomic DNA isolation ...
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Were we able to create vitamin B12 in past?

All herbivores produce vitamin B12 de novo. Gorillas, for example, are "vegans" so I suppose some human ancestor was also herbivore. Have we ever been B12 self-producers? If so, why have we lost that ...
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Can I use yogurt to prevent the growth of black mold?

I have a mold problem in my bathroom. There is a narrow groove between the top of my tub and the wall it is attached to where a black gunk repeatedly grows. I can shoot the material out with a spray ...
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How to characterise a protein family in a putative genome island?

We have sequenced the genome of 200 bacterial strains belonging to the same species, a swine bacterial pathogen. In a previous work, it was observed that a protein family of adhesins is present in ...
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Can different strains of Rhizobium share an infection thread or symbiosome?

Rhizobium infection can be triggered at root hairs of legumes, creating infection threads. Can these infections threads be colonized by more than one type of Rhizobium (e.g. Fixing and non-fixing)? ...
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Effect of flask position in shaking incubator affects growth kinetics

During some cultivation experiments for my bachelor thesis I noticed that in liquid culture my bacteria (E. coli) grew differently, according to the position in the shaking incubator. Because the ...
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Differential Scanning Calorimetry for bacterial membranes

I would like to study the freezing and melting of bacterial membranes and would like to use Differential Scanning Calorimetry (DSC) to obtain the glass transition temperature of the membrane. However, ...
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College Bio Research: Bacteria found on computer keyboards. [closed]

I am a college students brainstorming ideas on different topics that may be appropriate for on-campus research. What I came up with and I would like to give it a try is analyze the different types of ...
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Improving throughput of CFU plating?

In a separate question I've described my general experimental setup where I need to measure the number of live cells in a growing bacterial culture in a fairly rapid and high-throughput manner. In ...
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Are all single-celled organisms Bacteria?

I read that "Bacteria are one-celled organisms that can multiply by division", are all one-celled organisms bacteria or are there any more narrow definitions?
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Can viruses that normally infect eukaryotic cells also infect bacteria?

Can standard viruses infect bacteria? I'm not speaking of bacteriophage but typical RNA and DNA virus such as influenza and Ebola.
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What is the nature of plasmids? [closed]

What are the plasmids composed of? Are they composed of genomic or non-genomic DNA? I guess the answer is "genomic DNA" but I'm not sure.
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What is the mechanism by which bactericides kill bacteria? [closed]

"Bactericides kill bacteria". What is the meaning of "killing" in this context, and how exactly is this "killing" happening chemically?
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Heterocyst function in cyanobacteria and its localization

1.What is the function of heterocyst? 2.Where it is present? A heterocyst I think may be a protecting organ in cells!
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Why is it that bacteria and fungi cannot develop resistance against essential oils?

I wonder why bacteria and fungi cannot develop resistance and mutate agains essential oils? For example, some fungi get killed by oregano oil. Being not a biologist, I hope my question is not so ...
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What is the maximum amount of different bacteria in the drinking water in Europe?

I am looking for a statistical amounts which are allowed. Some students say it is 0 for all bacteria, which I think is false. I found this USA source. I found there Total Coliforms (including fecal ...
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Is it possible for bacteria to acquire family-wide antibiotic resistance?

If there is bacteria, lets say Enterococci that is sensitive to: Amoxycillin-Clavulinic acid Ampicillin-Sulbactam Ofloxacin Ciprofloxacin Is it possible to ...
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How to get conservation of vaccine candidates between hundreds of bacterial strains?

We have identified vaccine candidates via an ex-vivo RNA-seq approach. Next step would be to perform conservation of these candidates (about 20) between multiple bacterial strains (about 200). I would ...
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How might one determine if bacteria are nitrogen fixing or not?

If you had an isolate bacteria sample how would you determine if they were nitrogen fixing?
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Does E. coli survive at any pH level?

Does E. coli survive at any pH level? If I was to incubate it in agar of different pH, would it still form a bacterial lawn as it's called?
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How is bacterial plasma membrane made?

Eukaryotes have ER which manufactures plasma membrane of cells. How is prokaryotic plasma membrane made ? What is the pathway and which enzymes are involved ?
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What are artifacts in microbiology?

What kind of objects can be qualified as artifacts? How do we distinguish such objects?
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What does NaOH do for the plating of Bacillus subtilis?

A book called Molecular Biological Methods for Bacillus called for plating Bacillus subtilis cells on LB-agar plates with the following components: A general-purpose medium containing per litre: ...
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What is the effect of antibiotic chloramphenicol on bacterial size?

When low amounts of antibiotics like Chloramphenicol is added in a bacterial culture, the steady-state growth rate decreases but what happens to the steady-state size? Can anybody please refer me to ...
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What are the evidence that all life today descended from a common ancestor (LUCA), and which organisms (if any) challenge the concept?

If I understand correctly, the concept of the LUCA (last universal common ancestor) is based on the hypothesis that archaea and bacteria share common ancestry. In the realm of mathematics, the same ...
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Do aerobic bacteria precipitate in a liquid culture?

In most books aerobic bacteria is shown growing only at the top of a static liquid culture tube? Shouldn't have precipitated instead of floating on the surface?
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I don't wash my hands and never get sick. Any theories? [closed]

I have only been sick a few times in my life(I'm 21 and probably 3-4 times) and even when I am sick I don't feel that bad and I am over it within a day or two. My family has always given me slack for ...
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Gene transfer between Gram positive and Gram negative bacteria?

How does transfer of genetic material occur between Gram positive and Gram negative bacteria?