A subdivision of microbiology dedicated to the study of bacteria.

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Risks in bacterial phage therapy

I just finished reading J. Craig Venter's book Life at the Speed of Light: From the Double Helix to the Dawn of Digital Life. The book is a little over a year old now, and Venter has an optimistic ...
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82 views

To what extent is it possible to understand if a bacteria can produce a protein? (in silico only!)

I have to answer a question about a sub-task of my thesis: Can the bacteria X produce the protein Y? I searched of course Google and BLAST. There is no data supporting that this specific bacteria ...
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33 views

Property of E.coli harboring F element

From Genetics by Ursula Goodenough E.coli harboring an F element are endowed with a number of phenotypic traits : 1.They are sensitive to infection by ssRNA phages and certain ssDNA phages. ...
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163 views

Why do peas change colour?

I am doing an experiment on preservation of peas. The first notable change to the peas over a period of one week is that they have changed colour from bright green to a dull colour... Why is this so?...
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303 views

Expression of plasmid genes

Are plasmid genes always expressed? If so, then isn't a bacterium wasting it's resources in expressing genes (like antibiotic resistance) which are not required in "normal" conditions? If not, then ...
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90 views

How do plasmids protect themselves against restriction enzymes?

How do plasmids protect themselves from restriction enzymes released by bacteria (i.e., against bacteriophages)?
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42 views

Do toxin producing strains of E. coli constantly produce toxin or only under certain conditions?

Background E. coli is prevalent everywhere but only some strains produce toxins harmful to humans, such as E. coli O157:H7. Questions 1) Would anyone know if these strains are always producing ...
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39 views

Comparative effectiveness of antibiotics against colonies and growing bacteria

Appropriate antibiotics usually eliminate bacteria. However are the same antibiotics as effective against bacteria that have grown to form a colony?
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35 views

How does the peptidoglycan layer of a bacterial cell wall help prevent osmotic lysis? [duplicate]

I recently read that penicillin works by damaging the peptidoglycan layers of a bacterial cell wall causing osmotic lysis, which is when the bacteria cell bursts due to osmotic pressure. I just ...
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487 views

How is a bacterial strain defined?

When a species of bacteria is referred to by its strain, are they a clone of single founder or is a certain amount variation allowed?
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138 views

If all bacteria in human gut are dead, can they be there again?

If someone likes to eat chocolate, she may have gut bacteria which like chocolate according to some articles that I have read. http://www.theatlantic.com/health/archive/2014/08/your-gut-bacteria-want-...
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48 views

Are there antibiotics for all bacteria somewhere in the earth? [closed]

When I read about Penicillin, I found that it came from a green mold to protect itself. If all bacteria and/or molds in competition to survive, can we believe that there is an antibiotic for each ...
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30 views

Why would a bacterial population show initial growth when it is in unfavorable growth conditions?

This figure shows the anaerobe E. faecalis grown in aerobic conditions, E. coli grown in restricted conditions that are not specified. Why do they show an increase in abundance initially? The black ...
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51 views

Blood testing for chlamydia

I am working with the bacterium Chlamydia trachomatis. The main test for this species is the naat test, which is based on amplification. But I would like to know how the bacteria affects the blood ...
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38 views

Is it possible to merge the genomes of photosynthesizing and electron-transporting bacteria to make an electric cell?

I had an idea of making electric current from bacteria. There are some bacteria that can photosynthesize, and some others are capable of transmitting electrons through each other. Is it possible that ...
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385 views

Does E. coli survive at any pH level?

Does E. coli survive at any pH level? If I was to incubate it in agar of different pH, would it still form a bacterial lawn as it's called?
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996 views

What is the fastest growing bacterium?

E. coli has a generation time of about 20 min, and a single CFU will generate a colony a few millimeters in diameter after 16 hours of incubation. Is this the upper limit? How much faster can ...
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19 views

Are efflux pumps mutations or are they originally present in N. gonorrhoea?

I'll start by saying that I am no biologist. I am a math major trying to create a mathematical model for gonorrhea strain competition. My assignment is to create a model similar to a model that was ...
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380 views

Bacterial Conjugation/Horizontal Gene Transfer — how does the plasmid exchange work?

So according to a PPT I'm reading, bacterial conjugation works by the two bacteria joining pili and exchanging plasmids. So how exactly do the plasmids get across the gap? If I understand this ...
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37 views

Correlating Ki values of pesticides to bacterial growth

Four pesticides P1 to P4 are reversible inhibitors of an enzyme E that is essential for the growth of a bacterium B. Their Ki values are given in the table below. Each of these four pesticides is ...
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87 views

Why bacteria produce light?

After seeing this video I am curious to know why the free living bacteria produce light. What advantage will they have ? Or is it just an "unintentional" result of one of their pathways ?
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482 views

Blast a sequence against multiple databases

I would like to BLASTP a list of protein sequences in fasta format against multiple protein databases. Since I'm only interested in the first hit of each database, it is possible that BLAST result ...
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3answers
335 views

Assay for Beta-galactosidase activity in single cell microscopy

I'd like to be able to measure the activity of $\beta$-galactosidase in living cells with simple optical (maybe fluorescence) microscopy. Ideally I'd like to do a minimum of genetic engineering, and ...
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86 views

Are there any bacteria that can receive ultrasound signals?

I'm looking for an example of bacteria that could receive ultrasound (at any ultrasound frequency) signal and possibly perform some (re)action afterwards.
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162 views

Is iodised salt harmful to the lactofermentation process?

I was discussing sauerkraut recipes with someone on IRC a minute ago, and they warned me not to use iodised salt in the process. My first (and so far, the only successful) batch is one I made with ...
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487 views

What is a good Microbiology atlas for Bacteriology as online version?

I am looking for a online atlas for Bacteriology, the cost does not matter. I have been searching SpringerLink, and Amazon but no good online version found. What is a good atlas for Bacteriology as a ...
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17 views

Can 3% hydrogen peroxide be used as effective disinfectant and antiseptic?

I was curious to see whether 3% hydrogen peroxide can be used as antimicrobial and virucidal product for daily use in home. There are several disinfecting products in the market claim that use of ...
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31 views

After a bacterial infection is dealt with, do any of that colony of bacteria remain?

Suppose that I get a bacterial infection, which my immune system deals with. Are there any remnant bacteria, or are they all gone? Now consider the same scenario, but having been treated with a full ...
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26 views

Parafilm vs Foil for Retaining Plate Moisture while Allowing Gas Exchange

We often plate Streptomyces aureofaciens and grow them in a 30°C room. A post-doc I work with has always loosely wrapped the agar plates in aluminum foil to retain moisture and prevent cracking of the ...
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Glucose Oxidase, Condition to be Active?

I most recently was reading/watching information on honeys antibatical and antimicrobial properties. The enzyme Glucose Oxidase came up often as a main antimicrobial, yet it is inactive in honey. ...
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24 views

How do I “reinoculate” a petri dish with new bacteria?

Hypothetical question, intended to find a general procedure rather than case-specific recommendations: I have a petri dish full of growth medium with a large population of gram-negative, anaerobic ...
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71 views

Why do gram-positive bacteria cause respiratory diseases more frequently than gram-negative bacteria? [closed]

My theory is that aerobic respiration by gram positive bacteria thrives in ideal conditions of the lungs therefore creates respiratory diseases?
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47 views

What kind of organism grows in two-day-old lemon water? [closed]

I had a cup of lemon water on my desk that had been sitting out for 2-3 days. Today I took a sip without even realizing this. I looked at the water and it had something growing in it that looked like ...
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15 views

Growth charecteristics of Bacillus and Lactobacillus Species [closed]

Does anybody know weather the media (Hichrome Bacillus Agar) supports both Bacillus and Lactobacillus species, it is supposed to be the sample of the Lactobacillus plantarum spread on Hichrome ...
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31 views

Can oral bacteria be targeted by white blood cells?

Today I was told from a dentist that a leukocyte is much larger than a bad bacterium. So a leukocyte cannot remove bad bacteria when they hide in very places like the space between the gums and teeth ...
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40 views

Multiplication of bacillus subtilis

I have a supplement for use in horticulture. It is a powder that contains mostly subtilis with smaller amounts of a few other strains. Prior to horticultural application I would like to "multiply"...
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60 views

Why doesn't one develop immunity to bacterial STIs?

Why doesn't one develop immunity to STIs such as Chlamydia/Gonorrhea and Syphilis even after the first exposure and treatment with antibiotics?
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34 views

Bacteria surviving a β-lactam antibiotic

What changes can occur in the cell wall of a bacteria for it to survive a β-lactam antibiotic? I think that because a bacteria possesses peptidoglycan in its cell wall, they are β-lactam sensitive, ...
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25 views

How do microbes develop resistance to anitmicrobial peptides?

I would like to better understand how bacteria use the "strategy" of alternations to lipid A and membrane proteins in order to resist antimicrobial peptides of the immune system? It is my ...
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20 views

how to calculate dilution factor and CFU

I'm very confused I did a bacterial experiment where i added 5 grams to 70ml of distilled water that contains 10g of fresh meat. I left it in a sampler container over 24 hours then i cultured ...
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38 views

Is it worth to experiment with pro-biotics at home?

Let's assume that Joe has done some investigation on Internet and wants to see, if pro-biotics would help him. Now he goes to amazon.com and buys a pro-biotic product with excellent average rating. ...
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Microbiology- Pseudomonas aeruginosa, BSAC data

I tested Pseudomonas aeruginosaagainst various antibiotics, using Stokes and Kirby-Bauer. When I compared my results with BSAC data it was completely different (understandable) but why for a lot of ...
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percentage of animal diseases caused by bacteria?

I know this is a long shot, but what is the approximate percentage out of all the animal diseases that are caused by bacteria? One of the Q&A websites answer it as 90, but is there any conclusive ...
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49 views

Which Lactobacillales (Lactic acid bacteria — LAB) strains are capable of degrading starch?

I've been researching bacteria strains but am having trouble finding amylolytic LAB strains with amylolytic and lactic acid producing character. The only species I've found, that are capable of ...
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33 views

Enrichment of slow growing microbes: Large Test Tubes as an alternative?

Well, i would like to enrich bacterial and archaeal ammonia oxidisers from soil samples using Minimal Salt media. They are slow growing microbes. I would like to enrich them with the aim to produce ...
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90 views

What does NaOH do for the plating of Bacillus subtilis?

A book called Molecular Biological Methods for Bacillus called for plating Bacillus subtilis cells on LB-agar plates with the following components: A general-purpose medium containing per litre: ...
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Unsure why Synechococcus moves at angle in cuvette

I'd really appreciate your help. I'm working with Synechococcus. In my experiment, I have Synechococcus in a solution of milli-Q water. I illuminated it with a focused white light through a pinhole ...
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36 views

Requirements for soil sample transportation over long distance

I need to collected soil sample from agricultural area far away from my lab. It would required about a week for the sample to arrive to lab. I need the soil sample for genomic DNA isolation (...
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154 views

Were we able to create vitamin B12 in past?

All herbivores produce vitamin B12 de novo. Gorillas, for example, are "vegans" so I suppose some human ancestor was also herbivore. Have we ever been B12 self-producers? If so, why have we lost that ...
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49 views

Can I use yogurt to prevent the growth of black mold?

I have a mold problem in my bathroom. There is a narrow groove between the top of my tub and the wall it is attached to where a black gunk repeatedly grows. I can shoot the material out with a spray ...