A subdivision of microbiology dedicated to the study of bacteria.

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Comparative effectiveness of antibiotics against colonies and growing bacteria

Appropriate antibiotics usually eliminate bacteria. However are the same antibiotics as effective against bacteria that have grown to form a colony?
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How much genomic variation one usually find within a given bacterial species?

If I find the exact starting position (say 1152471) of the coding sequence of a given gene in the genome of a bacterium, is the genome of the bacterium in general stable enough so that I can expect to ...
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What does NaOH do for the plating of Bacillus subtilis?

A book called Molecular Biological Methods for Bacillus called for plating Bacillus subtilis cells on LB-agar plates with the following components: A general-purpose medium containing per litre: ...
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Comments on Experimental result regarding Anti-Bacterial soap

After using soaps, we rinse our hands with water. Do bacteria from the water attach themselves to our hands again? If we wipe our hands to cloth after washing our hands, do bacteria from the cloth ...
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Age-Dependent STD Infection

It seems that young females (comparing age 15 vs age 24) are 20x more likely to get chlamydia from a single unprotected sexual encounter with an infected male. What are the reasons for this? Are ...
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Are antibiotic-resistant bacteria necessarily less virulent than their original strain?

In the absence of antibiotic treatment, is there a reduction in the average severity of disease caused by resistant strains of pathogenic bacteria compared to wild-type ones? I would assume that ...
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After a bacterial infection is dealt with, do any of that colony of bacteria remain?

Suppose that I get a bacterial infection, which my immune system deals with. Are there any remnant bacteria, or are they all gone? Now consider the same scenario, but having been treated with a full ...
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Can Helicobacter pylori be eradicated naturally?

This question is not to discuss whether substances that have anti-H. pylori activity work or not, or to discuss the pros and cons of such but from a medical perspective. Assuming that one is able to ...
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How does the peptidoglycan layer of a bacterial cell wall help prevent osmotic lysis? [duplicate]

I recently read that penicillin works by damaging the peptidoglycan layers of a bacterial cell wall causing osmotic lysis, which is when the bacteria cell bursts due to osmotic pressure. I just ...
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“Most bacteria have at least one cell wall”

In the first answer to How do we find antibiotics? @Ashafix claims that Most bacteria have at least one cell wall Does this mean that there are bacteria with more than one cell membrane? Which ...
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Parafilm vs Foil for Retaining Plate Moisture while Allowing Gas Exchange

We often plate Streptomyces aureofaciens and grow them in a 30°C room. A post-doc I work with has always loosely wrapped the agar plates in aluminum foil to retain moisture and prevent cracking of the ...
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How do infectious bacteria determine when their numbers are high enough to attack a host?

When you get sick, you generally don't contract enough bacteria at once for them to succeed in battling your immune system, right? Their numbers must gradually increase in the host's body before they ...
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Glucose Oxidase, Condition to be Active?

I most recently was reading/watching information on honeys antibatical and antimicrobial properties. The enzyme Glucose Oxidase came up often as a main antimicrobial, yet it is inactive in honey. ...
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Why does cold kill humans but not bacteria? [closed]

Bacteria can continue like nothing happened after being exposed to low temperatures why doesn't this happen to humans as well? Why can't our metabolic machinery continue as normal?
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How can you develop a bacterial infection when taking antibiotics for another area of the body with a bacterial infection? [closed]

How can you develop a bacterial infection when taking antibiotics for another area? For example, "if someone was prescribed an antibiotic for a bacterial infection of the throat and while taking the ...
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Lysis of bacteria by complement system

The complement system creates pores in cell membrane which leads to influx of lots of water thereby causing lysis of bacterial cell. But what I fail to understand is that if bacteria have cell walls ...
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How are antibiotic resistant bacterial infections treated?

For example, how are infections of antibiotic resistant strains of MRSA, Streptococcus, or Gonorrhea treated?
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Do bacteria grow on pure dry glucose?

I've accidentally touched pure glucose with my bare hands (fingers to be specific), which was intended for cell-culture. I'm worried that bacteria from my skin may start to grow on the glucose and ...
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Can bacteria, viruses, and cancer cells be destroyed by resonance?

Radiotherapy has been used to treat cancer. Can the resonances by coordinated electromagnetic waves (and/or other forms of waves), of various frequencies, amplitudes and pulse rates, directed from ...
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Serological assays measuring antibody response

Given that an appropriate immune response to a bacteria may be thwarted in an individual, including not producing all of the antibodies which are known to occur in people who have been infected, or ...
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Did the Great Oxygenation Event also cause a mass extinction?

Its usually assumed that the Great Oxidation Event around 2.3 bya caused a great extinction of anaerobic life on earth. But as I was reading Nick Lane's book, ...
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Link between NaCl and sulfide reducing bacteria

Could there be any link between the consumption of NaCl and sulfide-reducing bacteria? In my study on mice, sulfide-reducing bacteria (SRB) are more abundant, in high-salt-fed group compared to the ...
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Did Cyanobacteria alone create the oxygen in Earth's atmosphere 3.5 billion years ago?

In other words, is the conveniently close-to-Californian-Universities pools of such representative of accepted theory. With the immediate follow-up question, when did the much more impressive amount ...
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What did cyanobacteria gain from photosynthesis?

As far as I understand, cyanobacteria began performing photosynthesis long before plant cells as we know them arrived on the scene. But cyanobacteria do not seem to use polysaccharides in the same way ...
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Does drinking alcohol having UTI may help to kill the bacteria?

It's well known that ethyl alcohol kills bacteria. And amount of alcohol in urine may be detected by EtG tests (as long as 3-4 days after intake). Does drinking alcohol in reasonable amount by ...
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What is the difference between an antibiotic and an antibacterial?

Concerning medicine, what are the differences between antibiotics and antibacterials?
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Why doesn't the rest of the body have something like the “blood-brain” barrier to protect itself from pathogens?

According to Wikipedia: "The blood–brain barrier acts very effectively to protect the brain from most pathogens". This is because the pathogens cannot pass through the tight junctions of the ...
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I don't want to know what bacteria is called but if the bacteria from unborn babies have benifits on health? [duplicate]

This question is not the same as the duplicate. I was told that when babies are born they are born with bacteria in their stomach. When a child takes antibiotics are there unique bacteria destroyed ...
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Why does a semi-set curd not set completely after a round of churning?

I left a pot of milk to turn into curd. While it did look like it had turned to curd since it started coming off the sidewall of the pot, but after having churned it, I realized that it wasn't set ...
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How do I “reinoculate” a petri dish with new bacteria?

Hypothetical question, intended to find a general procedure rather than case-specific recommendations: I have a petri dish full of growth medium with a large population of gram-negative, anaerobic ...
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Why doesn't one develop immunity to bacterial STIs?

Why doesn't one develop immunity to STIs such as Chlamydia/Gonorrhea and Syphilis even after the first exposure and treatment with antibiotics?
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Multiplication of bacillus subtilis

I have a supplement for use in horticulture. It is a powder that contains mostly subtilis with smaller amounts of a few other strains. Prior to horticultural application I would like to "multiply"...
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Why do gram-positive bacteria cause respiratory diseases more frequently than gram-negative bacteria? [closed]

My theory is that aerobic respiration by gram positive bacteria thrives in ideal conditions of the lungs therefore creates respiratory diseases?
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37 views

Detection of bacteria to measure quality of water

I have been working on a science project were I have built a water filter. To prove that it works I would need to test if there are bacteria in a sample which has been treated vs a sample that hasn't ...
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Is this bacteria or fungi?

I've been growing bacteria on an agar plate and after putting the dishes in the incubator, this is what I saw. I'm new to bacteria identification, and is this just contamination by fungi or is this ...
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What prevent us from synthesizing a cell from “scratch”?

In this recent article "Design and synthesis of a minimal bacterial genome", they created a minimal cell with only 473 genes. However, they didn't synthesize all of the necessary components of the ...
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How do minute changes in an organism, as a result of evolution, get carried over to next generation

The theory of evolution states that random variations in organisms occur to better suit their surroundings. We take for example a bacteria's flagella. There's a bacteria who has a tube kind of ...
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Gas from bacteria that's not methane

Is gas produced by bacteria always mainly methane? Or, are there bacteria out there that produce some biogas composed mainly of hydrogen, natural gas, propane, butane?
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What kind of organism grows in two-day-old lemon water? [closed]

I had a cup of lemon water on my desk that had been sitting out for 2-3 days. Today I took a sip without even realizing this. I looked at the water and it had something growing in it that looked like ...
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Growth charecteristics of Bacillus and Lactobacillus Species [closed]

Does anybody know weather the media (Hichrome Bacillus Agar) supports both Bacillus and Lactobacillus species, it is supposed to be the sample of the Lactobacillus plantarum spread on Hichrome ...
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Do plant and animal bacterial pathogen differ too much?

I will be involved in a plant disease produced by a bacterial pathogen, Acidovorax citrulli. I've previously worked with animal pathogens such as Haemophilus parasuis. Are there any big differences ...
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How to obtain bacteria samples at home?

As the original question went from hold to closed, I thought I would write up a more appropriate question. How should one go about getting bacterial samples to look at under a microscope at home? ...
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Can oral bacteria be targeted by white blood cells?

Today I was told from a dentist that a leukocyte is much larger than a bad bacterium. So a leukocyte cannot remove bad bacteria when they hide in very places like the space between the gums and teeth ...
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How can I make sure my UV-purificator kills bacteria?

I'm going to show a UV-purificator for water on science fair. How can I easily check if purificator kills most of the bacteria? I need a quick and possibly easy method.
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Are there bacteria that respire anaerobically in aerobic conditions?

There are facultative aerobic bacteria that switch to aerobic respiration in an aerobic state, but are there any organisms that would still perform anerobic respiration even when shifted to aerobic ...
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When streaking microbes on a plate to test growth after uv exposure, does how I streak make a difference?

I've been working on what I think might be a special strain of E. coli, I've been streaking it on LB agar plate and exposing it to varied times of UVC via the germicidal hood. I'm wondering if how I ...
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What is the difference between a bacterial artificial chromosome (BAC) and a plasmid?

Is it just that a BAC is generally larger and artificially constructed? Or are there any other differences?
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Blood testing for chlamydia

I am working with the bacterium Chlamydia trachomatis. The main test for this species is the naat test, which is based on amplification. But I would like to know how the bacteria affects the blood ...
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Reasons for variable quality virus production

I am making MSCV retrovirus, using DH5α cells to amplify a plasmid of interest, qiagen's midiprep kit to purify out the plasmid, then 293ft cells to produce the retrovirus, and 3t3 cells to test MOI. ...
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Effects of antibiotics on bacterial growth [closed]

I will do an experiment on effects of antibiotics on antibacterial growth and I will use three antibiotics: ampicilin, chloramphenicol and streptomycin. To make a hypothesis I must have a guess on ...