Tagged Questions

A subdivision of microbiology dedicated to the study of bacteria.

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How to identify genes required for biofilm formation

This is just a homework question: Q: Let’s assume that there is an unknown set of E. coli genes that are required for biofilm formation. Describe a genetic experiment you could perform to try to ...
-3
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1answer
41 views

Nature of plasmids [closed]

What are the plasmids composed of?? Genomic DNA or non-genomic DNA ? I think, the answer should be "genomic DNA"...but am not too sure...
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0answers
42 views

Development of contagiousness after death [closed]

Thought experiment: In an unfortunate accident in the future my heart will stop exactly at 0:00:00 due to a cause which kills me instantly but leaves my body intact (poison, electric shock, whatever). ...
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2answers
39 views

Are all single-celled organisms Bacteria?

I read that "Bacteria are one-celled organisms that can multiply by division", are all one-celled organisms bacteria or are there any more narrow definitions?
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0answers
32 views

How often does bacterial transformation happen?

I have been reading: M. Dröge, A. Pühler, W. Selbitschka, "Horizontal gene transfer among bacteria in terrestrial and aquatic habitats as assessed by microcosm and field studies", Biol. Fertil. ...
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0answers
15 views

Effect of flask position in shaking incubator affects growth kinetics

During some cultivation experiments for my bachelor thesis I noticed that in liquid culture my bacteria (E. coli) grew differently, according to the position in the shaking incubator. Because the ...
2
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1answer
31 views

Are added yogurt cultures harmful to the gut?

I have read somewhere that the live culture added to yogurt acting as "healthy" gut bacteria are actually synthetic and harmful. Any truth to this? If so, what are some alternatives to getting good ...
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2answers
57 views

Can Hepatitis be caused by Bacteria?

There are many information about viral hepatitis. But it is interesting for me, can bacteria cause the hepatitis too?
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1answer
33 views

Will an 11W UVC lamp kill bacteria?

If I shone a UVC lamp (11W) on something (say for example a plastic phone case) for an hour, would that kill off a noticeable amount of bacteria? That is, if I were to go over the case with a cotton ...
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0answers
20 views

Is there any way the food industry can benefit from biofilms?

I realize all of the disadvantages, but I am wondering if the food industry can actually benefit from the formation of biofilms.
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1answer
55 views

Does every wave length of UV kill bacteria?

Will every wave length of UV work for killing bacteria? So will UV A, UV B and UV C all work? Or will only the shortest of the three, UV C, work? Also, will the average UV lamp help against bacterial ...
2
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0answers
16 views

Can two Hfr strains of E. coli conjugate?

Genetics textbooks (and some internet searching) yield abundant examples of Hfr strains conjugating with F- cells, but these sources are surprisingly silent regarding the results of an Hfr ...
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2answers
107 views

Why does botulinum toxin seem to be more dangerous to humans than to other mammals?

Various mammals seem to get away with eating parts of carcasses that we would prefer to not even touch, and that we assume will make us sick. Because of that, I assume botulinum toxin is more ...
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2answers
249 views

How does isopropyl alcohol disinfect less in higher concentration?

Isopropyl alcohol can be used as disinfectant. For increasing concentration of it in water, the effect as disinfectant increases, and then decreases again. Typical concentrations for use as ...
2
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0answers
49 views

Can diseases be transmitted wirelessly? [closed]

According to the recent research about Electromagnetic Signals from Bacterial DNA (A. Widom, J. Swain, Y. N. Srivastava, S. Sivasubramanian) it seems there is a possibility of a "wireless" version of ...
44
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1answer
5k views

Do bacteria die of old age?

I know that the cells of mammals at least stop dividing when they are old, and then die a programmed cell death. Then other cells have to replace them. But in a bacterial colony, each cell ...
0
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1answer
29 views

Why is it that bacteria and fungi cannot develop resistance against essential oils?

I wonder why bacteria and fungi cannot develop resistance and mutate agains essential oils? For example, some fungi get killed by oregano oil. Being not a biologist, I hope my question is not so ...
3
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3answers
68 views

Is there a specific word for bacterial death?

What do you call it when a bacterium dies? Cellular death is apoptosis, necrosis, and bacterial is ...? I don't simply want to write in a paper that it - well dies!
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2answers
114 views

Can viruses that normally infect eukaryotic cells also infect bacteria?

Can standard viruses infect bacteria? I'm not speaking of bacteriophage but typical RNA and DNA virus such as influenza and Ebola.
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1answer
67 views

Does E. coli survive at any pH level?

Does E. coli survive at any pH level? If I was to incubate it in agar of different pH, would it still form a bacterial lawn as it's called?
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0answers
19 views

Is there a natural defense against an increase in E.Coli level in natural water systems?

I was recently on an Oceanology coliform collection vessel in the Long Island Sound and I found out that human waste contains E.Coli and when not properly treated the bacteria can run off or ...
1
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1answer
31 views

How long does it take for commercial fertilizers to break down? [closed]

Sometimes we buy carrots that are abit bitter, it's the taste of fertilzer. Once i put a tomato in the fridge, and when i ate it, it was bitter like shweppes, and i figured that was fertilizer also. ...
2
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2answers
48 views

Why isn't Bubonic Plague as virulent as it once was?

Although I am admittedly not anywhere near an expert on any topic, I still have a hard time understanding how Black Death and Bubonic plague are one in the same. Since plague still exists, with news ...
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3answers
125 views

What is the mechanism of oxygen uptake in E. coli?

How does E. coli uptake oxygen? Most of the literature I found is concerned with response to oxygen level supplied in the medium, as opposed to how much is actually transported inside. Can they shut ...
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1answer
56 views

What is the fastest growing bacterium?

E. coli has a generation time of about 20 min, and a single CFU will generate a colony a few millimeters in diameter after 16 hours of incubation. Is this the upper limit? How much faster can ...
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1answer
47 views

What are the dimensions in angstrom of bacteria? [closed]

I'm going to take a picture of a bacteria but I am unsure how large it is. What are the dimensions in angstrom of bacteria?
8
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1answer
215 views

Can a bacteriophage be used to treat bacterial diseases?

Some bacteriophages reproduce using the lytic cycle which ends with the destruction of the host bacterial cell. I was wondering if theoretically this could be used theraputically to treat bacterial ...
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0answers
9 views

Differential Scanning Calorimetry for bacterial membranes

I would like to study the freezing and melting of bacterial membranes and would like to use DSC to obtain the glass transition temperature of the membrane. However, I am unsure where to find such ...
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1answer
1k views

Calculation of the bacterial growth rate from a spectrophotomer growth curve

Typically the microbial growth in liquid cultures is monitored by turbidity. Data is obtained with a spectrophotometer to measure optical density at 600nm. The slope of the bacterial kinetic curve in ...
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1answer
15 views

Are efflux pumps mutations or are they originally present in N. gonorrhoea?

I'll start by saying that I am no biologist. I am a math major trying to create a mathematical model for gonorrhea strain competition. My assignment is to create a model similar to a model that was ...
3
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1answer
40 views

Could the growth rates of Vibrio vulnificus be affected by an oil spill?

I live on the Gulf Coast, and Vibrio vulnificus has recently made the news due to an increase in the number of reported infections. Naturally, it has people around here worried. I live in an area that ...
2
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1answer
35 views

What makes certain obligate anaerobes viable in fermentation starter cultures?

If Propionibacterium are obligate anaerobes (to wit, poisoned by oxygen), what makes 'Dairy' or classical propionibacteria (e.g. P. shermanii, P. jensenii, P. acidicpropionici, et al) viable in ...
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24 views

how to measure sugar concentration in growth medium

I am growing up bacteria in a flask and was wondering how do I track the changes of sugar concentration from the growth medium in the flask. Thanks for the help
3
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1answer
84 views

How does sucrose protect bacterial cells in lysozyme solutions?

I have a microbiology question. When we put bacterial cells in sucrose solution with concentration higher than 0.5M we observe plasmolysis - the cytoplasmic membrane detaches from the cell wall due ...
0
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1answer
52 views

To what extent is it possible to understand if a bacteria can produce a protein? (in silico only!)

I have to answer a question about a sub-task of my thesis: Can the bacteria X produce the protein Y? I searched of course Google and BLAST. There is no data supporting that this specific bacteria ...
5
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1answer
366 views

How does heat shock transformation work?

What exactly happens when competent cells like DH5ɑ are heatshocked with DNA present? How does the DNA get inside the cells? Specifically, why are all the steps necessary? What if you heatshock right ...
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0answers
34 views

What causes the odor when a container is sealed with moisture inside?

I'm not sure if this is a biology or chemistry question since I don't know the cause of it (though I think it is bacteria or fungal related). I'm just wondering what creates that awful odor when a ...
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0answers
62 views

Transportation of bacteria

I would like to take an apparatus containing bacteria to another country (Bolivia) from the U.S.. How do I determine whether I am allowed to do this? The bacteria are: ...
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1answer
74 views

Blood consumption

Is consumption of blood more "dangerous" compared to meat? There was a news-article about unnatural chemicals found in the blood of mothers. This reminded me about a question I have pondered upon ...
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3answers
106 views

Restriction endonucleases are found in?

Quoting from : Scientific American July 1975 The Manipulation of genes by Stanley Cohen : Restriction endonucleases (and modification methylases) are widespread in microorganisms; genes for ...
6
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2answers
236 views

How Sex Evolved ? and when?

As I know evolution comes bit by bit mutation by mutation How sex evolved which requires a major change in at least two individuals one to become male and one to become female ? When that happened ...
3
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1answer
50 views

What is the film that covers the tongue?

What is the film that covers the tongue in the mornings, even after brushing the teeth and tongue the night before and why does it have color variations? Do the different colors mean anything?
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1answer
21 views

How is bacterial plasma membrane made?

Eukaryotes have ER which manufactures plasma membrane of cells. How is prokaryotic plasma membrane made ? What is the pathway and which enzymes are involved ?
0
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1answer
27 views

Property of E.coli harboring F element

From Genetics by Ursula Goodenough E.coli harboring an F element are endowed with a number of phenotypic traits : 1.They are sensitive to infection by ssRNA phages and certain ssDNA phages. ...
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2answers
58 views

Functioning of EDTA

I know that EDTA chelates metal ions. It weakens bacterial cell wall and inactivates the DNases. What is the reason why it can do so ? I guess it can inactivate DNases by altering the ...
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1answer
53 views

Buy bacteria for experiment?

Where can I buy paracoccus denitrificans? I need it for an experiment. I cannot find online where I can purchase this.
3
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1answer
29 views

Functioning of BCG vaccine

I read (from Nature Volume507, S4–S7 (06 March 2014) : For reasons that are poorly understood, BCG protects only infants; it is ineffective in older children and adults. Its efficacy also ...
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3answers
96 views

How quickly is antibiotic resistance lost?

I would imagine the bacterial genome is highly conserved and limited in its space, but maybe I am wrong. If you were to take a strain of antibiotic resistant bacteria and kept them isolated, but fed ...
2
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0answers
49 views

College Bio Research: Bacteria found on computer keyboards. [closed]

I am a college students brainstorming ideas on different topics that may be appropriate for on-campus research. What I came up with and I would like to give it a try is analyze the different types of ...
2
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3answers
802 views

How do a viruses or bacteria survive outside the body long enough to spread?

Say I cough on my table, then someone else touches it and picks up something I've got... how is it that these things can live outside the body, how long can they manage it, and how long is generally ...