A subdivision of microbiology dedicated to the study of bacteria.

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What are the evidence that all life today descended from a common ancestor (LUCA), and which organisms (if any) challenge the concept?

If I understand correctly, the concept of the LUCA (last universal common ancestor) is based on the hypothesis that archaea and bacteria share common ancestry. In the realm of mathematics, the same ...
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Decomposition Rate of A Body In A Wet Environment

I have a photo of a decomposing dog which is in a water logged environment. My peers and I are debating how long that body has been decomposing. They are saying it is at least a year old. I however ...
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What bacteria shed harvestable energy from root zone organic matter?

I recently heard of a successful effort that harvests energy from soil at plants' bases due to apparently bacterial breakdown of wastes from the plants: Via photosynthesis a plant produces organic ...
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Generate T2/T1 Phage Resistant E Coli

I would like to generate T2/T1 phage resistant Stbl3 E Coli to use in virus production. Is there a plasmid somebody has used that confers resistance, or is this done another way?
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eradicating h.pylori naturally

This question is not to discuss whether substances with anti-h.pylori agents works or not, or the pros and cons of such but from a medical perspective. Assuming that one manage to control, reduce, or ...
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Do sulfate reducing bacteria help during the formation of the mineral dolomite in low temperature environments?

Do sulfate reducing bacteria help during the formation of the mineral dolomite in low temperature environments? Wikipedia says The actual role of bacteria in the low-temperature formation of ...
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Cheapest Way to Measure Germ Density

I would like to measure the surface density of Staphylococcus bacteria and Rhinoviruses (only those two, to be specific) within my home. What's the cheapest way? The textbook procedure is to: rub ...
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Unsure why Synechococcus moves at angle in cuvette

I'd really appreciate your help. I'm working with Synechococcus. In my experiment, I have Synechococcus in a solution of milli-Q water. I illuminated it with a focused white light through a pinhole ...
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Can two Hfr strains of E. coli conjugate?

Genetics textbooks (and some internet searching) yield abundant examples of Hfr strains conjugating with F- cells, but these sources are surprisingly silent regarding the results of an Hfr ...
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101 views

Transportation of bacteria

I would like to take an apparatus containing bacteria to another country (Bolivia) from the U.S.. How do I determine whether I am allowed to do this? The bacteria are: ...
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284 views

Image Processing Suite for bacterial microscopy: Schnitzcells or MicrobeTracker?

I am looking to start doing some work tracking the size and growth of individual bacterial cells in the microscope. In order to analyze the images I need software that can segment the cells, ...
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Are the intestinal flora substantially different within a diverticulum of the colon?

From the Wikipedia page for the vermiform appendix: This proposal is based on a new understanding of how the immune system supports the growth of beneficial intestinal bacteria, in combination ...
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Requirements for soil sample transportation over long distance

I need to collected soil sample from agricultural area far away from my lab. It would required about a week for the sample to arrive to lab. I need the soil sample for genomic DNA isolation ...
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101 views

Were we able to create vitamin B12 in past?

All herbivores produce vitamin B12 de novo. Gorillas, for example, are "vegans" so I suppose some human ancestor was also herbivore. Have we ever been B12 self-producers? If so, why have we lost that ...
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Can I use yogurt to prevent the growth of black mold?

I have a mold problem in my bathroom. There is a narrow groove between the top of my tub and the wall it is attached to where a black gunk repeatedly grows. I can shoot the material out with a spray ...
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28 views

How to characterise a protein family in a putative genome island?

We have sequenced the genome of 200 bacterial strains belonging to the same species, a swine bacterial pathogen. In a previous work, it was observed that a protein family of adhesins is present in ...
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Can different strains of Rhizobium share an infection thread or symbiosome?

Rhizobium infection can be triggered at root hairs of legumes, creating infection threads. Can these infections threads be colonized by more than one type of Rhizobium (e.g. Fixing and non-fixing)? ...
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Effect of flask position in shaking incubator affects growth kinetics

During some cultivation experiments for my bachelor thesis I noticed that in liquid culture my bacteria (E. coli) grew differently, according to the position in the shaking incubator. Because the ...
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Differential Scanning Calorimetry for bacterial membranes

I would like to study the freezing and melting of bacterial membranes and would like to use Differential Scanning Calorimetry (DSC) to obtain the glass transition temperature of the membrane. However, ...
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Improving throughput of CFU plating?

In a separate question I've described my general experimental setup where I need to measure the number of live cells in a growing bacterial culture in a fairly rapid and high-throughput manner. In ...
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What is the effect of antibiotic chloramphenicol on bacterial size?

When low amounts of antibiotics like Chloramphenicol is added in a bacterial culture, the steady-state growth rate decreases but what happens to the steady-state size? Can anybody please refer me to ...
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21 views

Do aerobic bacteria precipitate in a liquid culture?

In most books aerobic bacteria is shown growing only at the top of a static liquid culture tube? Shouldn't have precipitated instead of floating on the surface?