A subdivision of microbiology dedicated to the study of bacteria.

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Can Helicobacter pylori be eradicated naturally?

This question is not to discuss whether substances that have anti-H. pylori activity work or not, or to discuss the pros and cons of such but from a medical perspective. Assuming that one is able to ...
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242 views

What bacteria shed harvestable energy from root zone organic matter?

I recently heard of a successful effort that harvests energy from soil at plants' bases due to apparently bacterial breakdown of wastes from the plants: Via photosynthesis a plant produces organic ...
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45 views

Did the Great Oxygenation Event also cause a mass extinction?

Its usually assumed that the Great Oxidation Event around 2.3 bya caused a great extinction of anaerobic life on earth. But as I was reading Nick Lane's book, ...
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27 views

Are there bacteria that respire anaerobically in aerobic conditions?

There are facultative aerobic bacteria that switch to aerobic respiration in an aerobic state, but are there any organisms that would still perform anerobic respiration even when shifted to aerobic ...
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62 views

What is the advantage of using plant-derived antibacterials rather than bacteria-derived antibacterials?

So obviously we have a big problem with antibiotic resistance. Most of our antibiotics originate from bacteria themselves (or are synthetic variations on scaffolds which originate from bacteria). I ...
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27 views

How do I test if microbes have survived after dehydration?

I have a solution containing various bacteria and fungi. My aim is to place solution on filter paper, and wait until it dries. I then wish to test if the organisms have survived, either on the dried ...
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30 views

Generate T2/T1 Phage Resistant E Coli

I would like to generate T2/T1 phage resistant Stbl3 E Coli to use in virus production. Is there a plasmid somebody has used that confers resistance, or is this done another way?
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38 views

Do sulfate reducing bacteria help during the formation of the mineral dolomite in low temperature environments?

Do sulfate reducing bacteria help during the formation of the mineral dolomite in low temperature environments? Wikipedia says The actual role of bacteria in the low-temperature formation of ...
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128 views

Cheapest Way to Measure Germ Density

I would like to measure the surface density of Staphylococcus bacteria and Rhinoviruses (only those two, to be specific) within my home. What's the cheapest way? The textbook procedure is to: rub ...
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34 views

Can two Hfr strains of E. coli conjugate?

Genetics textbooks (and some internet searching) yield abundant examples of Hfr strains conjugating with F- cells, but these sources are surprisingly silent regarding the results of an Hfr ...
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139 views

Transportation of bacteria

I would like to take an apparatus containing bacteria to another country (Bolivia) from the U.S.. How do I determine whether I am allowed to do this? The bacteria are: http://www.atcc.org/Products/...
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371 views

Image Processing Suite for bacterial microscopy: Schnitzcells or MicrobeTracker?

I am looking to start doing some work tracking the size and growth of individual bacterial cells in the microscope. In order to analyze the images I need software that can segment the cells, ...
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29 views

After a bacterial infection is dealt with, do any of that colony of bacteria remain?

Suppose that I get a bacterial infection, which my immune system deals with. Are there any remnant bacteria, or are they all gone? Now consider the same scenario, but having been treated with a full ...
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15 views

Parafilm vs Foil for Retaining Plate Moisture while Allowing Gas Exchange

We often plate Streptomyces aureofaciens and grow them in a 30°C room. A post-doc I work with has always loosely wrapped the agar plates in aluminum foil to retain moisture and prevent cracking of the ...
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9 views

Glucose Oxidase, Condition to be Active?

I most recently was reading/watching information on honeys antibatical and antimicrobial properties. The enzyme Glucose Oxidase came up often as a main antimicrobial, yet it is inactive in honey. ...
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24 views

How do I “reinoculate” a petri dish with new bacteria?

Hypothetical question, intended to find a general procedure rather than case-specific recommendations: I have a petri dish full of growth medium with a large population of gram-negative, anaerobic ...
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31 views

Can oral bacteria be targeted by white blood cells?

Today I was told from a dentist that a leukocyte is much larger than a bad bacterium. So a leukocyte cannot remove bad bacteria when they hide in very places like the space between the gums and teeth ...
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38 views

Multiplication of bacillus subtilis

I have a supplement for use in horticulture. It is a powder that contains mostly subtilis with smaller amounts of a few other strains. Prior to horticultural application I would like to "multiply"...
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58 views

Why doesn't one develop immunity to bacterial STIs?

Why doesn't one develop immunity to STIs such as Chlamydia/Gonorrhea and Syphilis even after the first exposure and treatment with antibiotics?
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34 views

Bacteria surviving a β-lactam antibiotic

What changes can occur in the cell wall of a bacteria for it to survive a β-lactam antibiotic? I think that because a bacteria possesses peptidoglycan in its cell wall, they are β-lactam sensitive, ...
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25 views

How do microbes develop resistance to anitmicrobial peptides?

I would like to better understand how bacteria use the "strategy" of alternations to lipid A and membrane proteins in order to resist antimicrobial peptides of the immune system? It is my ...
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20 views

how to calculate dilution factor and CFU

I'm very confused I did a bacterial experiment where i added 5 grams to 70ml of distilled water that contains 10g of fresh meat. I left it in a sampler container over 24 hours then i cultured ...
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38 views

Is it worth to experiment with pro-biotics at home?

Let's assume that Joe has done some investigation on Internet and wants to see, if pro-biotics would help him. Now he goes to amazon.com and buys a pro-biotic product with excellent average rating. ...
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16 views

Microbiology- Pseudomonas aeruginosa, BSAC data

I tested Pseudomonas aeruginosaagainst various antibiotics, using Stokes and Kirby-Bauer. When I compared my results with BSAC data it was completely different (understandable) but why for a lot of ...
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12 views

percentage of animal diseases caused by bacteria?

I know this is a long shot, but what is the approximate percentage out of all the animal diseases that are caused by bacteria? One of the Q&A websites answer it as 90, but is there any conclusive ...
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48 views

Which Lactobacillales (Lactic acid bacteria — LAB) strains are capable of degrading starch?

I've been researching bacteria strains but am having trouble finding amylolytic LAB strains with amylolytic and lactic acid producing character. The only species I've found, that are capable of ...
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30 views

Enrichment of slow growing microbes: Large Test Tubes as an alternative?

Well, i would like to enrich bacterial and archaeal ammonia oxidisers from soil samples using Minimal Salt media. They are slow growing microbes. I would like to enrich them with the aim to produce ...
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24 views

Unsure why Synechococcus moves at angle in cuvette

I'd really appreciate your help. I'm working with Synechococcus. In my experiment, I have Synechococcus in a solution of milli-Q water. I illuminated it with a focused white light through a pinhole ...
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35 views

Requirements for soil sample transportation over long distance

I need to collected soil sample from agricultural area far away from my lab. It would required about a week for the sample to arrive to lab. I need the soil sample for genomic DNA isolation (...
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148 views

Were we able to create vitamin B12 in past?

All herbivores produce vitamin B12 de novo. Gorillas, for example, are "vegans" so I suppose some human ancestor was also herbivore. Have we ever been B12 self-producers? If so, why have we lost that ...
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49 views

Can I use yogurt to prevent the growth of black mold?

I have a mold problem in my bathroom. There is a narrow groove between the top of my tub and the wall it is attached to where a black gunk repeatedly grows. I can shoot the material out with a spray ...
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67 views

How to characterise a protein family in a putative genome island?

We have sequenced the genome of 200 bacterial strains belonging to the same species, a swine bacterial pathogen. In a previous work, it was observed that a protein family of adhesins is present in ...
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16 views

Can different strains of Rhizobium share an infection thread or symbiosome?

Rhizobium infection can be triggered at root hairs of legumes, creating infection threads. Can these infections threads be colonized by more than one type of Rhizobium (e.g. Fixing and non-fixing)? ...
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97 views

Effect of flask position in shaking incubator affects growth kinetics

During some cultivation experiments for my bachelor thesis I noticed that in liquid culture my bacteria (E. coli) grew differently, according to the position in the shaking incubator. Because the ...
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20 views

Differential Scanning Calorimetry for bacterial membranes

I would like to study the freezing and melting of bacterial membranes and would like to use Differential Scanning Calorimetry (DSC) to obtain the glass transition temperature of the membrane. However, ...
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55 views

Improving throughput of CFU plating?

In a separate question I've described my general experimental setup where I need to measure the number of live cells in a growing bacterial culture in a fairly rapid and high-throughput manner. In ...
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15 views

Lysis of bacteria by complement system

The complement system creates pores in cell membrane which leads to influx of lots of water thereby causing lysis of bacterial cell. But what I fail to understand is that if bacteria have cell walls ...
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8 views

Serological assays measuring antibody response

Given that an appropriate immune response to a bacteria may be thwarted in an individual, including not producing all of the antibodies which are known to occur in people who have been infected, or ...
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17 views

Did Cyanobacteria alone create the oxygen in Earth's atmosphere 3.5 billion years ago?

In other words, is the conveniently close-to-Californian-Universities pools of such representative of accepted theory. With the immediate follow-up question, when did the much more impressive amount ...
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40 views

Link between NaCl and sulfide reducing bacteria

Could there be any link between the consumption of NaCl and sulfide-reducing bacteria? In my study on mice, sulfide-reducing bacteria (SRB) are more abundant, in high-salt-fed group compared to the ...
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7 views

Why does a semi-set curd not set completely after a round of churning?

I left a pot of milk to turn into curd. While it did look like it had turned to curd since it started coming off the sidewall of the pot, but after having churned it, I realized that it wasn't set ...
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43 views

Is this bacteria or fungi?

I've been growing bacteria on an agar plate and after putting the dishes in the incubator, this is what I saw. I'm new to bacteria identification, and is this just contamination by fungi or is this ...
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21 views

Do plant and animal bacterial pathogen differ too much?

I will be involved in a plant disease produced by a bacterial pathogen, Acidovorax citrulli. I've previously worked with animal pathogens such as Haemophilus parasuis. Are there any big differences ...
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6 views

Reasons for variable quality virus production

I am making MSCV retrovirus, using DH5α cells to amplify a plasmid of interest, qiagen's midiprep kit to purify out the plasmid, then 293ft cells to produce the retrovirus, and 3t3 cells to test MOI. ...
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19 views

How to promote denitrifying microbe activity

I'm an amateur fresh-water aquarist looking at the problem of nitrate reduction (into largely-inert nitrogen gas) in a small-scale aquarium environment. The process of turning the byproducts of fish ...
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16 views

Are Betaproteobacteria more 'simple' than Gammaproteobacteria?

More generally, are all proteobacteria named in order of complexity (Alpha, Beta, Gamma, Delta, Epsilon)? If so, would it be right to say that Betaproteobacteria can survive in lower nutrition / more ...
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19 views

Feasibility of “smart effective microorganisms”

This might be a stupid question as I have little to no knowledge of biology, but I would really like to find out if it would be feasible to make such a system as a way of preventing infections. I ...
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31 views

Need clarification on a sentence regarding vesicle-mediated translocation

I've chanced upon a passage that is not entirely clear to me: In addition to conjugation, transformation and transduction, other less well recognised mechanisms of DNA uptake occur in nature, ...
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What is the effect of antibiotic chloramphenicol on bacterial size?

When low amounts of antibiotics like Chloramphenicol is added in a bacterial culture, the steady-state growth rate decreases but what happens to the steady-state size? Can anybody please refer me to ...
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Where does P. gingivalis come from?

This is a bacterium that is associated with periodontitis - inflammation of the gums that can lead to tooth loss. I asked my PI this question. He didn't get it. He said well, it's communicable ...