The study of chemistry within the scope of biology: the compounds that occur and the reactions involving them in living organisms.

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Results of fermenting bakers yeast, Sugar and different categories of fruit peels

Based on this recipe I made three Jars of the fermented solution each containing: Papaya Peels Lemon+ Pomegranate peels Pineapple Peels In addition to above recipe I added 1 large tbsp of ...
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1answer
43 views

Why is lead dangerous? [closed]

Lead is known to be bad for your health for a long time, but I can't find a explanation why is it so. In school they teach that heavy metals are poisonous, and they are trapped inside our bodies. I ...
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2answers
30 views

How does one determine intracellular concentration?

The TL;DR version Is there a fast way to determine what the cell environment of a particular cell (E.g RBC) is? (in terms of solute/ionic concentration) I'm not sure if the question belongs here, ...
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1answer
33 views

How is the rate of transcription influenced by temperature?

How is the rate of transcription influenced by temperature? More precisely, I am looking for an article who quantitatively measured the rate of transcription of an "average gene" and show how this ...
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1answer
48 views

Influence of temperature on protein binding and decay rates

For computer modeling purposes, I am looking for some referenced quantitative measurements of the effect(s) of temperature on the dynamic of biochemical reactions. Question In particular, my ...
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1answer
104 views

Integration of several environmental signals

I am looking for examples of different functions that are good fit to how signals are computed in order to respond to the environment. Let's make my question more copmrehensible with an example... ...
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1answer
55 views

Influence of temperature on transcription, protein binding and decay rates

I am the kind of biologist who doesn't know much about molecular genetics and about the dynamic of biochemical reactions. Question My question concerns the influence of temperature on the dynamic of ...
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23 views

Enzyme Assay - pectinase

During assaying an enzyme at high temperature, the substrate (Pectin) is degraded by the high temperature rather than by enzyme, so, how can I minimize degradation of the substrate by the temperature? ...
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1answer
131 views

Why do we absorb vitamins better from whole foods than from pills?

We can't rely on pills to provide us our nutrients because we don't absorb the nutrients from the pills as effectively. Presumably this is because pills are lacking components that are required for ...
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3answers
130 views

Why can't you just take a vitamin? Why you need a healthy diet on top of that?

From what I understand, your body needs certain amounts of vitamins and minerals to maintain health. Why can't we just take enough pills to obtain these vitamins and minerals?
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1answer
45 views

What causes skewed ladder bands on an agarose gel?

I frequently ran into an issue where the bands of my ladder become skewed. What causes this? Here's an example: 0.7% agarose gel, 100V, 60 minutes. Same problem occurs with 1%. I loaded 10 ul of a 20 ...
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1answer
27 views

What is the expected effect of pH on the activity of a fungal pectinase?

I am working on an enzyme assay for a fungal pectinase.I assayed the enzyme in different buffers from pH 1-12.5 However,the enzyme has good activities starting from pH1-10.5. Is it possible to have ...
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0answers
24 views

Fatty Acid Synthesis of Fats Longs than 16 C

I understand that the human body when performing Fatty Acid Synthesis can synthesize only until C16 (palmitate). However the ER has desaturases and elongases. I know that elongases are used to add ...
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1answer
67 views

Enzyme Assay, absorbance change to concentration change

I have a few issues with this question. An enzyme assay is performed and the kinetic data graphed. The y-intercept of a Lineweaver-Burk plot is 0.6 min/ΔA. The substrate being assayed has a molar ...
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1answer
33 views

What is the biding site code recognized by the parts of the spliceosome

Another question about another Youtube video. At 0:50, the splicing process begins to remove the non-coding section of the DNA (intron), so the different parts of the spliceosome attach to the borders ...
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1answer
47 views

What is an effective method for putting on blue rubber butyl stoppers?

They look like this. We have to do a lot of gas sampling and these are really difficult to assemble. I've been scouring the internet for advice but there are none. Do you have experience with ...
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2answers
216 views

Why is oxygen needed for the electron transfer phosphorylation?

I understand that oxygen is the acceptor of electrons and hydrogen ions during the electron transfer phosphorylation, the last step off the ATP-producing aerobic respiration. But why? Aren't there ...
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2answers
32 views

Concentration dependent cellular processes

Are there any famous biological processes that depend strongly on a chemical concentration reaching a particular value, like some sort of switch? E.g. if concentration of chemical x reaches ...
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1answer
41 views

Why do oncogenes show genetic dominance?

As we know that tumor suppressor gene causes cancer only when both the alleles are recessive in nature.But in case of oncogenes if only one allele is dominant it can cause cancer.Why in case of ...
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1answer
38 views

Mechanism of DNA gyrase inhibitor

Why DNA gyrase inhibitor, such as Nalidixic acid and Norfloxacin, do not stop gyrase from cutting DNA helix but only prevent them from decatenation replicating DNA?
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2answers
54 views

Bohr effect in respiration

Would I be correct in saying that the Bohr effect is ONLY related to the concentration of [H+] in the tissues. Obviously the concentration of carbon-dioxide can contribute to this concentration by ...
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45 views

Poly Unsaturated Fatty Acid

PUFAs spontaneously oxidize, speed up the process of glycation, since too much glucose and they also decrease mitochondrial respiration - more oxygen and CO₂, less lactate and PUFA also promote ...
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1answer
41 views

naturally occuring fatty acids

The double bonds in naturally occurring fatty acids present in our body are in cis configuration while the enzymes for beta oxidation can act on trans double bond. Then what will be the significance ...
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1answer
68 views

What organism most efficiently converts a given quantity of mass into heat?

Question I am trying to answer: In the popular film The Matrix, heat given off by humans is harvested as an energy source. I wondered, if this were possible, would humans be a good organism to ...
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1answer
36 views

About molecules and their shape

When a molecule is being used in some biochemical process it has a certain 3-d structure. Is this maintained throughout it's usage or can one specific molecule change it's 3-d shape while it is being ...
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4answers
74 views

Why is too much glucose harmful?

I learned the citric acid cycle in biotechnology school and how cells work; about ADP and ATP and how the Cellular respiration (C6H12O6 + 6O2 -> 6CO2+6H2O) works. I am interested in understanding why ...
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24 views

Can non-carbonated energy drinks boost alcohol intoxication?

I just read news article about banning caffeine in alcohol drinks and about that energy drinks increase the urge to drink alcohol. Energy drinks cause sugar intoxication - answer about it here ...
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0answers
55 views

amino acid containing branched side chain

Branched-chain amino acid are degraded by branched chain alpha-keto acid dehydrogenase complex. These amino acids aren't degraded in liver because of the absence of this enzyme(in liver).Why the ...
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1answer
27 views

bacterial cell wall degradation in humans

can human degrade the D-amino acid present in bacterial cell wall, I'm confused about it i have read somewhere that human can do so.If yes than why we need antibiotic to kill bacteria???If it is not ...
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1answer
61 views

Scale up of Bacterial growth from 250mL to 1-5L?

I am currently growing up a specific strain of E.Coli with a knockout in 40mL of growth medium (LB) in a 250mL shaker flask... My ultimate goal is to scale up this process to a 1-5L large scale setup ...
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0answers
30 views

Isoprene and Acrolein emissions of wood burning, insect burning and smoldering tobacco cigarette

I'd like to identify what makes a fume more or less toxic, above all those from commonly smoked organic stuff: tobacco, cannabis, comparatively to less common ones. For that, we can focus on 2 gas ...
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0answers
25 views

What chemical form of vitamin A is in chicken's egg yolk?

After doing some existing background research I am confused as to exactly what, in an unfertilised chicken egg's yolk, offers what we intake as 'Vitamin A' upon eating it. Plants typically produce ...
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3answers
119 views

More entropy: Atom or Macromolecule? [closed]

A question that appeared on my last exam was : Which of the following has greater entropy A) An atom B) A macromolecule The question doesn't specify anything else(i.e. type/size of atom or ...
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0answers
89 views

Why doesn't honey spoil quickly? [duplicate]

Why is it that honey can last for decades and not spoil like other foods? Is there any chemical in honey which gives rise to this amazing feat?
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2answers
117 views

Effect of steroid hormone on specific cells?

As steroid hormones can pass through the plasma membrane by simple diffusion because they are lipid derived hormones, it means that they are capable of passing through every cell of our body, BUT why ...
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1answer
57 views

Mesoporous silica particles as anti cancer drug delivery method

If this question is a little off topic for Biology, I'm sorry. I've recently read a lot about mesoporous silica nanoparticles as a promising drug delivery method. The huge range of customizability of ...
4
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1answer
88 views

Question about Monofunctional glycosylases

I am learning about Base excision repair mechanism. I understand the main idea how glycosylases are working, but I am trying to understand this with much more details. I found a paper where they are ...
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1answer
60 views

How to recognize a conserved motifs of the protein

I would like ensure that my reasoning is correct. Assuming that I know the aminoacids sequence of the protein of interest. I can't say anything about the structure looking only at the aminoacids ...
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2answers
82 views

Significance of synthesis of D-glucose in plants..?

why plants can only synthesize D-glucose why not L-glucose along with D glucose. I know it very well that plants have only enzymes which can synthesize D-glucose but Why not they have enzymes which ...
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1answer
26 views

Formation of peptide bond..?

Proteins peptide bond is made by condensation process in which a molecule of water is released and according to this process it is not favorable in water systems than how peptide bond is formed by ...
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1answer
114 views

which signalling pathway is involved in cancer?

Columnar epithelial cells from the colonic mucosa are studied to identify abnormalities in cell signaling pathways. Abnormal epithelial cells from colonic adenocarcinoma are shown to have a mutation ...
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2answers
142 views

Effect of testosterone hormone on cell signalling and behaviour?

A steroid, testosterone was injected in female body which led to development of secondary sexual characters but these characters were not developed over night (Response was very slow). What could be ...
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2answers
346 views

Why does Hunger lead to the aggressive behavior?

I have observed that frequently when people are hungry; they tend to get angry more easily on pointless issues. Does this mean that our fight or flight response is more active when a person is hungry? ...
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4answers
168 views

Specificity of Protein Kinases in Signaling Pathways..?

In most of the signaling pathways the activated receptor when activates Protein Kinase through the action of secondary messenger, then these protein kinases almost always phosphorylate on the specific ...
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2answers
482 views

Essential amino acid codons

Essential amino acids also have codons.Still they are needed by humans through diet?Will those codons do synthesize amino acids for some other purposes in humans or not?If so, what is the purpose?e.g? ...
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1answer
21 views

Primers on Dystonia

Where can I get a good biochemical overview of dystonia? I will be working in a research lab that focuses on dystonia and I would like to not be clueless.
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1answer
30 views

Is the sense for salt depending on the electrolyte level in our body?

I often noticed, after (heavy) physical activity like cycling, running, swimming or working an isotonic drink (to recover the electrolyte level) tastes less "salty" compared to when drinking it before ...
5
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1answer
129 views

Equation for accurate prediction of PCR yield

It is a cliche of freshman biology labs to point out that "every cycle of PCR doubles the DNA, so the yield will be $2^{cycles}$ times the template amount". However, if this were true, 1 ng of ...
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0answers
34 views

What causes the odor when a container is sealed with moisture inside?

I'm not sure if this is a biology or chemistry question since I don't know the cause of it (though I think it is bacteria or fungal related). I'm just wondering what creates that awful odor when a ...
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2answers
89 views

alpha tubulin molecular weight problem

Is there any academic reference that shows α-tubulin is around 50-55 kDa? The only thing I found is some data sheets from companies. I need the real reference.