The study of chemistry within the scope of biology: the compounds that occur and the reactions involving them in living organisms.

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Which method would more accurately help to identify the unknown concentration of a protein sample between the A280 and the Bradford methods?

I quantified my protein using the standard Bradford method and the A280 methods and obtained values that were far off from the theoretical value of the protein of interest, and therefore was wondering ...
13
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1answer
1k views

What is the most condensed form of (stored) energy used in biology?

When considering how organisms store energy for later use, I was wondering what substance known in Biology is best for compacting the most energy in the smallest space(volume-wise). With my (limited) ...
2
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0answers
48 views

Zinc and brewer's yeast

I'm hoping for some information relating to yeast nutrition (specifically Saccharomyces cerevisea) in beer fermentation. Zinc is well-known to be necessary for yeast cultures to perform successful ...
2
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0answers
62 views

How does estrogen influence collagen synthesis?

Through what mechanisms does estrogen interact with collagen synthesis? Especially in the context of elevated estrogen levels and genesis of purpura simplex .
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2answers
93 views

What is a triglyceride?

I'm confused on what a triglyceride is, from what my text book it says its a type of gylercide, then from a website it said Glycerides can be subdivided into two categories. The first group, the ...
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1answer
44 views

Is there a biological environment that we can we assume glutamate exists as glutamic acid?

In the body we almost always assume that glutamate exists as glutamate rather than glutamic acid. It is so commonly glutamate yet glutamic acid and glutamate share the abbreviations of Glu and E. From ...
2
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2answers
84 views

How to preserve leaves between sampling (collection) and analysis?

Suppose I spend a day in a forest, collecting different kinds of leaves, which I would like to analyze under a compound microscope. Now, obviously, measures should be taken to preserve the leaves some ...
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1answer
94 views

Is using Hidden Markov Models to find homologues sensible in abstract, short sequences?

HMM alignment tools like hhpred excel at finding subtle homologues of folded proteins that simpler scoring techniques (such those used in BLAST algorithms) would miss. I am only looking at a small ...
2
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1answer
47 views

Why is the resting potential of a neuron so close to the equilibrium potential of K⁺?

I know this has something to do with the K+ leak channel. I just don't understand how. I know that 3 Na+ are pumped out for every 2 K+ pumped in. This makes the cell interior net negative. I know ...
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1answer
40 views

Are there U-rich transcriptional pause sites?

I've heard a statement in a talk that U-rich sequences cause transcriptional pausing. The intuitive explanation for this was that the UTP concentration is lower in the cell than the ATP concentration. ...
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0answers
24 views

Explanation of sequence motif diagram given in wikipedia [duplicate]

can someone please explain me this graph?? I did not get what information content is in this graph means. Does the size of the letter indicate that higher chance of that letter to be present in that ...
3
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0answers
34 views

How does beta branching stop alpha helices from forming?

I am told that beta branching interferes with alpha helix formation. Problem is that I don't see how beta branching has anything to do with alpha-helix formation. Beta-branches are on the outside of ...
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0answers
38 views

Cannot conjugate Biotin-labeled DNA to Streptavidin-labeled solid surface

I have been trying to immobilize DNA by the bioconjugation of biotin and streptavidin, but I cannot get this work. I added EDC and streptavidin to COOH ...
2
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0answers
20 views

Which biosynthetic pathways take place in the plastid and were are they located?

I know that the isoprenoid, jasmonate, glucosinolate, fatty acids, chlorophyll, starch, and aromatic amino acid syntheses are located in the plastid. But I don't know if they are located in the ...
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1answer
36 views

Dehydration from caffeine, alcohol and other

Tea, Coffee, Beer, Coke etc… I wonder if the benefit from amount of fluid we get from them is bigger or smaller then the handicap of dehydration. In other words it is worth to drink them if we want ...
3
votes
1answer
83 views

Dehydration by a tea, coffee, beer etc

For the long time I am trying to find out if the following is truth: They say that some beverages dehydrates. I heard that about tea (sometimes green, sometimes black, sometimes both), coffee, beer ...
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2answers
51 views

Conversion of glucose to glycogen

Why is excess glucose, as a glycogen, stored in only a limited amount as compared to the lipid in our body? Why is mature glycogen arranged (polymerized) in 12 tiers polymer?
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0answers
17 views

What would happen to a body in an astronaut suit over time if floating free? [duplicate]

I'm not sure if this is best in Biology or Physics, but on Earth we decay to skeletons and then eventually to dust. What would happen in space in a space suit?
3
votes
1answer
76 views

How long does it take to form a peptide bond?

What is the time taken to form a peptide bond in vivo or in vitro? It isn't mentioned in my course on protein structures. I was just curious to find out if any time scale is known? Given that ...
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1answer
40 views

How much mass does a person lose in a day through breathing and perspiration?

Water and carbon are lost through perspiration and breathing. How much mass is lost in this way in a single day for a typical person?
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33 views

What are the biological mechanisms of candy causing nausea?

As I sit here after eating too much chocolate, I wonder: What are the biological mechanisms behind eating too much candy candy causing nausea in a healthy individual? Is it a spike in blood sugar, ...
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3answers
223 views

True or false & fill in the blanks

A ribosome is where amino acids are linked together by peptide bonds. A virus is NOT considered prokaryotic because it does not have a membrane. The nucleotide ATP is one of the most used energy ...
-3
votes
1answer
61 views

Could we engineer humans to behave like insects? [closed]

First off, though complex, I'm assuming insects are at an earlier evolutionary stage than humans. That being said, could humans be genetically engineered to behave like insects? I'm not talking about ...
2
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1answer
43 views

Glycerol 3-phosphate nomenclature

Why is Glycerol 3-phosphate named in such way? Shouldn't it be named as Glycerol 1-phosphate by proper IUPAC- nomenclature?
3
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0answers
43 views

Why is ATP the main nucleoside triphosphate used to exchange energy? [duplicate]

Out of all of the nucleoside triphosphates what makes ATP the most used? Is it its structure? The amount of energy it contains? Why is GTP not used as much? What is the deal with the other nucleoside ...
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2answers
180 views

Why is water a polar molecule? [closed]

Also, what is the definition of polar in this case? (9th Grade) Does it have to do with the bonds it makes?
2
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1answer
57 views

Is it possible that a set of functionally related proteins in a pathway fulfill different functions? [closed]

Could it be that a given pathway of enzymes (or proteins in general) may fulfill different purposes in a cell by for shifting partners? Say protein A activates B, B activates C and C has a specific ...
7
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1answer
137 views

Why is leucine amino acid used the most in proteins and tryptophan the least?

The amino acid leucine, is used in proteins more than others. Leucine with 9.1 percent (its average in more than 1.150 different proteins) is used most and tryptophan with 1.4 percent is used less ...
5
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1answer
100 views

Are there ways to speed up the growth of plants?

I'm interested in what humans can do to speed up the rate of growth in a plant. I'm interested in both the context of home gardening and large-scale, institution-backed projects. Obviously, optimal ...
10
votes
1answer
247 views

Why doesn't HDL cause diseases like LDL? [duplicate]

LDL particles pose a risk for cardiovascular disease when they invade the endothelium and become oxidized, since the oxidized forms are more easily retained by the proteoglycans. A complex set of ...
0
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1answer
50 views

How is oxygen and carbon dioxide exchange mediated by hemoglobin? [closed]

Oxygen is transferred by hemoglobin from the lungs to tissues, while carbon dioxide is transferred by hemoglobin from tissues to lungs. How is this regulated bidirectional transfer mediated?
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2answers
116 views

What is the importance of urea in mass spectrometry?

What is the importance of urea in mass spectrometry? We use 8M urea to FASP our proteins prior to mass spectrometry. What is the significance of using 8M urea? and how does it affect the proteins?
10
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3answers
189 views

Formation of Life

Originally, life evolved from non-living matter. Why is life only generated from other life nowadays, and why doesn't it evolve from inanimate matter, like it did originally billions of years ago, ...
1
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1answer
26 views

What controls Fructose regulation in plants?

Sugars are found ubiquitously in plants and are regulated. For sucrose it's pretty straightforward - it's basically kept at a low ish level, and put into storage or other intermediate compounds. ...
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0answers
49 views

How to measure concentrations of different metals from the environment?

Suppose that different kind of metals and chemicals (e.g. magnesium, sodium, copper) got transmitted to environment e.g. as a cause of an accident. How would measure the levels of those metals and ...
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1answer
47 views

Taste of salt water

I had a question relating to chemistry and biology, so please tell me if this is suited better for the Chemistry website. My question was, if water dissolves salt by separating the two ions, the Na+ ...
2
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1answer
22 views

Relative sweetness

I have noticed that when I eat something sweet, then afterwards, I eat something else that is sweet, the second sweet food is not as sweet as it usually is. I am pretty sure many others have a similar ...
4
votes
1answer
290 views

Besides hemoglobin, what proteins are present in red blood cells?

I knew that mature red blood cells (RBCs) lacked nuclei, but I wasn't aware until just now that they also lacked ribosomes and mitochondria. Most cells in the human body all contain a common laundry ...
3
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1answer
120 views

How do CO₂ and carbonic acid (H₂CO₃) work in buffering the blood?

Been struggling with this for the past few days even after reading half of the acid base tutorial here, if someone could help me that'd be great. What I don't understand is how HCO3- is supposed to ...
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1answer
109 views

How does salinity affect the bonds of an enzyme?

According to my textbook, "Too much or too little salt can interfere with the hydrogen bonds that hold an enzyme in its three-dimensional shape". I know that NaCl is held together by an ionic bond, ...
2
votes
1answer
67 views

How do catalase and other antioxidants neutralize free radicals?

In learning about how cofactors are essential to proper enzyme function, my textbook mentioned catalase and its relation to the human body. According to my textbook, catalase is similar to hemoglobin ...
5
votes
1answer
73 views

How does the body “know” how to metabolize foreign substances

As a freshman biochemistry major, I find myself befuddled by our body's ability to perform complex chemical reactions to break down foreign substances. I can see how we would evolve explicit pathways ...
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2answers
64 views

Binding of Multivalent Antibody to mutiple epitopes?

A multivalent antibody molecule such as Immunoglobulin M Immunoglobulin A etc bind to more than one antigens or epitopes but I am confused about that wheather these multivalent antibodies bind to ...
4
votes
1answer
80 views

Do plants have preference for the form of nitrogen as nutrient?

In the nitrogen cycle (ecology), it is usually described that plants can use nitrogen in the form of ammonium (NH4+) and nitrate (NO3-). Do plants prefer one form of nitrogen over the other?
2
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1answer
38 views

Can plants directly use sulfuric acid?

In the explanation of the sulfur cycle, it is often said that sulfur moves from the atmosphere to the ground by acid rain in the form of sulfuric acid. Can plants directly use sulfuric acid to ...
2
votes
3answers
91 views

Increasing/decreasing signal intensity in Western blotting

So I was in class and my professor was explaining western blotting. It seems as though there's more than one way to increase or decrease the signal intensity. He presented us the challenge to discover ...
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1answer
44 views

Anesthetics, specifically inhaled anesthetics

I have had a look at previous inhaled anesthetics and many of them appear to be fluorocarbons. What could be the mechanism behind fluorine's anesthetic properties? Is it the specific bonding pattern ...
0
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1answer
37 views

Can the same protein be represented by primary,secondary,tertiary structure? [closed]

As my book says: Protein can be represented by 4 structures. The primary structure represents the main chain and positions of amino acid residues. But actually, protein is not a rod but rather a ...
0
votes
1answer
116 views

What is the charge on oligonucleotide 5' pGpGpApCpT 3' at pH 7.00?

What is the charge on the nucleotide 5'pGpGpApCpT 3' @ pH 7.00? I thought adenine has 1 NH group and Guanine has NH and ...
2
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1answer
94 views

How to manage fat-gluten oxidation in celiac disease?

Assume you have been in non-gluten diet 20 years. You built mass 8 mass with substances containing gluten (endogenous gluten stored in fat). Then, celiac disease is diagnosed. You have been 3 ...