The function of chemistry within the scope of biology; the study of the compounds that occur, and the reactions involving them, in living organisms.
2
votes
0answers
34 views
Do humans have chemosensors for nutrients or chemicals?
I'm reading about chemoreceptors on Wikipedia, and see that the typical ones are mentioned: taste, smell, co2. I would like to learn more about the other kinds of chemoreceptors that humans may ...
2
votes
0answers
63 views
Virucides - Herbal and otherwise, for HHV6 type virus or any inner-cell virus
Does anyone know where I can find a reliable list of virucides (not anti-virus) that can be used by humans? This virucide must be able to affect viruses that are already inside the cells. St John's ...
1
vote
2answers
226 views
How are proteins formed?
Somewhere, I have read that we need to consume proteins to make amino acids to make new proteins. What does it suggest? How do we make proteins from proteins?
1
vote
1answer
43 views
Formation of ammonia from air and moisture
On extended bicycle trips I sometimes slept under a plastic sheet, which helped with mosquitoes on hot nights. But there was a noticeable build-up of ammonia which made this almost impossible for more ...
1
vote
1answer
142 views
Which concentration of BSA is recommended for dynamic light scattering experiments?
What is the recommended concentration of BSA to be mixed with water so as to prepare a very dilute solution in order to clearly study the intensity-intensity correlation with single scattering ?
I ...
1
vote
2answers
66 views
PEG-silane treatment: why incubate for 18 hours at 60 degrees Celsius?
I am conducting a biochemistry-related experiment and I have been unable to understand a step which is commonly performed.
My aim in this step is to apply a PEG (Polyethylene glycol) silane layer.
...
1
vote
1answer
78 views
Chemical structure prediction
I'd like to do chemical structure prediction using a known molecule formulas. I'm familiar with de novo protein structure prediction, but are there any programs which will go from formula to structure ...
1
vote
2answers
53 views
Does the Urea Cycle exist in invertebrates?
Do invertebrates (like Drosophila and C.elegans) have a urea cycle?
1
vote
1answer
39 views
What is the use of futile reaction cycling such as Fruc-P to Fruc-BP?
One rate-limiting step of glycolysis is the conversion of Fructose-Phosphate (Fruc-P) to Fructose-Bisphosphate (Fruc-BP), catalysed by Phosphofructokinase (PFK). The reaction involves hydrolysing one ...
1
vote
1answer
31 views
How do animals/plants change for each season?
The question pretty much sums it up. How do animals/plants change for each: Spring, Summer, Autumn, and Winter?
A detailed explanation on distinct behaviour and appearance changes would be nice.
1
vote
1answer
69 views
How exactly does marijuana damage brain cells?
I've heard that THC can cause permanent damage to brain cells. I've also heard this reffered to anti drug propaganda. Another theory i've read is that temporary effects reduce intelligence but long ...
1
vote
1answer
58 views
What is the biochemical reason for mental fatigue?
Is it known exactly why the brain needs sleep? What's dropping low / going high when we experience mental fatigue? I can see why low glucose could result in mental fatigue, are other reasons known?
1
vote
1answer
56 views
What are the biochemical interactions between human body and Polyethylene?
Our waterways and oceans are showing increased levels of plastic contamination. These plastics are breaking down into smaller pieces. When one goes to the beach and plays in the surf they often ...
1
vote
1answer
84 views
How to manufacture different sized micelles in nano -scale?
I am trying to answer q5:
"How can you manufacture micelles in A) nanometerer -scale B) and in ten nanometer -scale?"
My Thinking
Observations and some thinking
Oil in ...
1
vote
0answers
19 views
Relationship between biomass and net primary productivity
I want to do validation on simulated net primary productivity. But I don't have measured NPP data, Eddy covariance flux measurement data. I have only field measurement biomass data. So can I directly ...
1
vote
0answers
34 views
How do omega-3 fatty acids reduce cholesterol at the molecular level?
I have heard that omega-3 fatty acids found in fish are a good way to prevent/reduce cholesterol problems.
My question is how do omega-3 fatty acids do that: what are they doing on a chemical level ...
1
vote
0answers
19 views
What is the translation termination efficiency in mammalian cells?
When I express proteins in bacteria I put at least two stop codons at the end of the gene to increase the termination efficiency. Is this the case in eukaryotic cells too? If I put a single stop codon ...
1
vote
0answers
15 views
Can the sulphur content of biological material be reliably calculated from fat, fibre and protein contents (Weender Analysis)?
An often-used analysis of feedstocks for lifestocks is the Weender analysis, which basically divides the volatile solid content into fats, fibre and protein, as well as N-free matter. I found no ...
1
vote
0answers
25 views
How to wash the column during protein purification with GST tag?
I have been working with GST tagged proteins for the last 4 years and after loading the cell lysate into the column I was washing it with 20-30 column volumes of PBS and sometimes my proteins were ...
1
vote
0answers
33 views
Why does trans fat decrease hdl's?
I understand that cis fats increases hdl's, and unsaturated fat increases ldl's, but trans fat increases hdl's and it decreases ldl's. Why does trans fat do this?
1
vote
0answers
18 views
Glutamine analysis
I use to run a method to analyse all the aminoacids in a food sample. For that I have to hydrololyse the sample and in the last stage of the method I read the amino acids with a ion exchange ...
1
vote
0answers
42 views
Solid phase use in HIV rapid tests
I have another question in regards to my HIV test research. The rapid tests like Orasures Oraquick contains a strip of synthetic peptides that are used to represent proteins found in the envelope ...
1
vote
0answers
72 views
Is eating salt dangerous for the brain?
I read somewhere that eating a lot of salt will raise blood pressure, which will in turn damage brain tissue. This is because water molecules gather around salt ions, thus making it harder for the ...
1
vote
0answers
50 views
When should endocytosis inhibitors be used in cell binding assays?
I'm beginning to do some cell-binding assays and I would like for my proteins to not be endocytosed by my mammalian cells. Typical suggestions are for the cells to be kept on ice and that the binding ...
0
votes
2answers
90 views
Why do living organisms replicate itself or procreate
Why do living organisms spontaneously replicate itself or "procreate" (my understanding is that it does).
From a uni-cellular and micro-organism point of view. Is there some sort of stimulant in the ...
0
votes
1answer
197 views
Is breathing in deodorant fumes dangerous for my brain?
I noticed that I sometimes get dizzy when I accidentaly breathe in too much Axe. Also, I find the smell of alchohol rich deodorants quite unpleasant. But, I was wondering, is it actually dangerous? ...
0
votes
1answer
22 views
Definition of Dye- Reduction Test?
Can some one give a simple explanation or definition on what a dye-reduction test is.
0
votes
0answers
19 views
How do cells slowly degrade 5,5'-dibromo-4,4'-dichloro-indigo?
5,5'-dibromo-4,4'-dichloro-indigo is the product of X-gal cleavage, often used as a reporter with B-galactosidase. I've made the (unreferenced) observation that it seems as though the blue product ...
0
votes
0answers
55 views
What are usable Sum formulas for Proteins?
I'm looking for something like this: $C_{13}H_{25}O_7N_3S$
. Obviously it's not about knowing the exact shape or composition of a specific protein, but knowing the relative contents of major chemical ...
0
votes
0answers
46 views
What metabolite intermediate will accumulate in a mammalian cell-free extracts capable of glycolysis if lactate dehydrogenase is inhibited? [closed]
I know the reaction proceeds as follows:
Pyruvate accepts the electrons from NADH to form lactate. What would be the intermediate in this reaction? Would it be NADH or NAD+?
0
votes
0answers
20 views
What are sulphur contents of diverse biological materials (mostly plants and excrements)?
This is a follow up to my unanswered question: How much sulphur can I assume in protein, in starch, in fat, in feces, in eggs, in meats? Or in specific plants? What ranges? Are there any tables about ...
0
votes
1answer
40 views
Plants without bacteria? is it theoretically possible?
I know from school, that all live on the Earth need bacteria as low-level "machines" that break down/extract/convert/produce chemical elements and combinations, other high-level organisms needed. But ...
0
votes
0answers
42 views
Bradford Protein Assay [closed]
Please would somebody help me to solve the following exercise:
Determine the protein concentrations of the four unknown samples (A,B,C,D) using the raw data obtained from a Bradford assay.
...
0
votes
0answers
61 views
What happens to proteins when they absorb UV? [closed]
I know that proteins strongly absorb UV at 280 nm.
I have 3 questions:
What happens to proteins when they absorb UV?
Do different proteins absorb different wavelengths ?
Does water absorb UV as ...
-2
votes
1answer
33 views
Good place to ask health based questions? [closed]
I like the development of biology.stackexchange.com, but it is still in an early stage and far from perfection. While we develop it further, could you guys help me finding places to ask questions ...
