The use of methods from the physical sciences to aid in the study of biological systems.

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1answer
57 views

What is the difference between rheobase and threshold?

Neuronal tissues can be excited by electrical stimulation. Two commonly encountered characteristics for electrically stimulating nerve cells is the threshold and the rheobase. My question is what the ...
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0answers
68 views

effect of pupil responses on the electroretinogram

The electroretinogram is generally considered to reflect the retinal processing of a light stimulus, typically a photoreceptor response followed by secondary neuronal activity. However, I am wondering ...
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0answers
54 views

Do voltage-gated calcium channels in earthworms relate to speed of neuron conduction?

We have a hypothesis that a drug may block the voltage-gated calcium channels (VGCC) in annelid worms. We are wondering whether there are any correlations between VGCCs and neuron conduction ...
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0answers
15 views

Criteria for electrophysiological threshold

As opposed to action potential thresholds (which are yes/no events), electrophysiological thresholds of compound action potentials are arbitrary. Mostly a certain noise level is picked and when the ...
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0answers
29 views

Kinesin-5 / cytoplasmic dynein spatial density distribution in neurons

is there some way to experimentally determine the density distribution of those two molecular motors in a Neuron? Fluorescence labeling would be impossible(?) as GFP markers would probably alter the ...
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0answers
19 views

Unexpected stoichiometry n in ITC experiments

I'm looking at the binding of a small molecule to several RNA constructs and tried to determine the binding constants. To do this I've performed a few ITC experiments on this system, the results were ...
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53 views

PQR files make from pdb2pqr are different to GROMACS

I have a pdb file and I would like to make a pqr one. In GROMACS, I use editconf -mead to produce pqr, with topol.top taken from pdb2gmx or use it to produce pdb file, then use pdb2pqr. Which one is ...