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38 views

How does one quantify T7 RNA polymerase transcription efficiency?

I am looking for a method which can help me calculate how many sequences one will get after transcription of 100-mer random sequences by T7 RNAP. The sequence pool has a known complexity and will be ...
1
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1answer
59 views

Why use DNA polymerase in making cDNA?

RT is capable of synthesizing a complementary dna strand ( as in HIV life cycle.) Then why is DNA pol used when cDNA (synthesizing the second strand of it ) has to be synthesized from mRNA ( For eg.to ...