Biotechnology is a field of biology which utilizes biological systems like microorganisms to produce valuable products, such as biofuels, drugs, and vaccines.

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Can I purify polyhydroxyalkanoates by heating the cells extensively?

Traditional methods of purifying polyhydroxyalkanoates (PHAs) and other bioplastics made by bacteria involve washing the cells with harsh chemicals or strong bases.I'm interested in maintaining the ...
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Why does bacillus thuringiensis produce bt toxin?

Background : B.thuringiensis produces an inactive crystalline toxin during sporulation which when ingested by an insect, gets activated and causes pore formation in gut , subsequently leading to death ...
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What should be the distance between plant promoter and gene?

I am trying to clone a rice gene under a different endogenous rice promoter.I will be cloning the CDS of the rice gene.So I wanted to know what is the minimum or maximum distance I should put between ...
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229 views

What specifically allows alpha-complementation in beta-galactosidase?

I'm familiar with the use of alpha-complementation of beta-galactosidase with the pUC alpha-peptide and the M15 lacZ gene product, and would like to hypothetically apply alpha-complementation in other ...
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Nutritious protein substance for vitamin enhanced crop?

I am not a bio or science major, but we have a subject, like an elective on biotechnology and we were tasked to think of a product that hasn't been invented yet. My groupmates and I thought of a ...
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Superpatients for Cancer resistance

I was reading an article on MIT Technology review about superpatients for low cholesterol that got me thinking whether such patients exist for cancer. The article is ...
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Would biotechnology in agriculture actually decrease biodiversity, as many people say? Does it have to?

I've heard the claim that because farmers would be using the same seed, you would end up with an entire crop population that could be susceptible to a disease. Like if you had a diverse population of ...
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37 views

Phage base Biosensor

What kind of biosensor is made by Phages? I need a list of published paper that used Phages for make LCR(inductance (L), capacitance (C), and resistance (R) of a component) biosensor for detection. ...
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Parazoa as part of sewage plant

in our zoology lecture our prof was talking about parazoa, the sponges, and that they are getting more and more in the focus of the biotech research. As he described there way of getting food, as ...
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Visualizing GFP in transgenic rice seeds?

I want to express GFP in rice as a method for differentiating transgenic from non transgenic rice seeds. I want to know if I can visualize them using a hand held UV lamp as the GFP I will be using has ...
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Which kit provides the highest yield and purity of extracted DNA from FFPE tissue samples?

I'm looking to extract DNA for sequencing from FFPE (Formalin fixed paraffin-embedded) tissue samples. I'd like to use a commercial kit that provides a high yield of good quality DNA.
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Is a neurostimulation implant for the soft-palate component of obstructive sleep apnea plausible?

There are currently ongoing clinical trials for neurostimulation implants designed to keep the tongue from blocking the airway in obstructive sleep apnea. The nerves for this are in the neck and ...
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Colormetric assay for a phenol product?

I am working on an assay for phenol products created by a colony of bacteria. I have searched up a paper that involves gibbs reageant but the protocol is kind of unclear. Does any of you know where I ...
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How are Stem Cells Produced for Stem Cell Based Therapies?

I was doing an initial search on google but couldn't come up with anything I'm looking for. I know were stem cells come from and a bit about their levels of differentiation. For example, embryonic ...
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33 views

How can site-directed mutagenesis be used to suppress the production of anti-antibodies?

In a previous post of mine, I asked how to supress the creation of anti-antibodies in vivo. In the answer, it was mentioned that site-directed mutagenesis could be used. Currently, I can't find mcuh ...