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How to induce a steady increase in cyctoplasmic [Ca2+] in HEK293T cells? And is there any simple marker/method to confirm it?

We are trying to find out whether increase or decrease of cytoplasmic calcium concentration affects the interaction of two proteins, using co-IP in HEK293T cells as a readout. In different forums and ...
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Has anyone modelled calcium oscillations using Matlab software? [closed]

I have been trying to model calcium oscillations using MatLab, but I cannot obtain the oscillation. I created a function where I put all the equations and used ode15s to call the function. I have ...
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Effect of extremely hard water on marine life?

When researching water quality standards, I found that a minimum and maximum was set for water hardness. Several sources also state that water can in fact be too hard. The question is how does hard ...
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Which hormones/factors affect Calcium absorption in the small intestine and its regulation?

I think the question is very broad and cannot find precise mechanisms. I am not sure about the precise mechanisms of the regulation. I think they are at least Vitamin D, diffusion and specific ...