The study of cells: their physiological properties, structure, environmental interaction, division, life cycle, and death, as well as the organelles they contain. Also known as cytology.

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Solubility of Forskolin in ethanol

I am interested in using forskolin in cell culture medium. Does anyone know how to make solution of 10 microM forskolin in 5% ethanol or less. I would like to avoid using DMSO as a solvent. Thank you. ...
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47 views

Exotic Cell Shapes

As far as I know, plant cell shapes are a difficult thing to pin down. However plant cells have cell walls and so can be very rigid. However the only plant cells I've seen have been either block ...
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67 views

Autophagy in eukaryotic cells

What is autophagy? How and under which circumstances is it used by the cell? I believe The reason for autophagy is some kind of recycling, am I right? But why does it occur in infections?
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Regarding cellular self-destruction

I heard and read telomere 'health' or 'length' ( if that's right ) has alot to do with cell 'health'. If telomere 'abilities' are 'restored' to a 'healthier' status then the cell it is in functions as ...
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22 views

How does one determine intracellular concentration?

The TL;DR version Is there a fast way to determine what the cell environment of a particular cell (E.g RBC) is? (in terms of solute/ionic concentration) I'm not sure if the question belongs here, ...
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33 views

What does the term 'epitope mapping' mean? [closed]

Epitope mapping means identifying the binding site of antibodies on the target antigen. This means that the site to be identified is part of the antigen and not antibody, am I right?
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31 views

What can thrombosis lead to?

I am thinking this question. Thrombosis can result in organisation of thrombus, sepsis thromboembolism, fibrinoid swelling adiposity. I fibrinoid swelling (edema) (4) can occur. Also, I think (3) ...
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82 views

Which of the cell types commonly found in mammals has the greatest number of mitochondria?

This is basically a fun question, inspired by this answer on scifi.se. So, which cell type will have the greatest number of mitochondria? Obviously, I am talking about wild type, healthy individuals ...
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172 views

Image Processing Suite for bacterial microscopy: Schnitzcells or MicrobeTracker?

I am looking to start doing some work tracking the size and growth of individual bacterial cells in the microscope. In order to analyze the images I need software that can segment the cells, ...
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22 views

How is saltatory conduction faster than conduction of unmyelinated fibers? [duplicate]

It's always the same explanation that currents are able to "hop" along Ranvier nodes instead of passing continuously along the axon making saltatory conduction more efficient than continuous ...
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54 views

Critical pO2 threshold for cell division?

I'm a physicist asking questions on an aspect of cellular metabolism, so excuse my biological ignorance! Hypoxia is a frequent complication of certain tumour types, and has quite an implication for ...
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177 views

which exact mechanism triggers the first cell differentiation after n divisions?

I would like to understand which mechanism triggers the first cell differentiation after n divisions. I read previous articles on SE and Wikipedia articles on cellular differentiation and ...
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88 views

Coiling of chromatids during cell division

What is exactly coiling of chromosomes? I just heard about the names i.e paranemic, plectonemic, orthostichious, anorthospiral. I have ecaxtly no idea of what phenomenon is this. Also what type of ...
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48 views

How to use mechanical microstrainer to extract tissue proteins from human?

Background: There are many methods to extract proteins form human tissues out there. The majority of them use an extraction buffer containing variable concentrations of detergents and protease ...
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37 views

Example(s) of reduced rate of mitotic progression?

Most species complete mitosis, and in particular the process of chromosome condensation, rather quickly, in a matter of minutes. Are there any known species that undergo mitoses with substantially ...
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46 views

Onset of Autophagy

Fasting and Intermittent Fasting (IF) have been proven to start autophagy (cellular self-digestion). How long does the average man and woman have to wait for autophagy to begin? Does diet prior to ...
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44 views

Similarities Between Cells?

Which of the following are usually identical between a lung cell and a brain cell, from the same person, assuming that they are normal (non-cancerous) cells? Circle your choice(s). ...
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69 views

Why is too much glucose harmful?

I learned the citric acid cycle in biotechnology school and how cells work; about ADP and ATP and how the Cellular respiration (C6H12O6 + 6O2 -> 6CO2+6H2O) works. I am interested in understanding why ...
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49 views

Functioning of EDTA

I know that EDTA chelates metal ions. It weakens bacterial cell wall and inactivates the DNases. What is the reason why it can do so ? I guess it can inactivate DNases by altering the ...
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1k views

What is the appropriate blender speed to maximize nutrition and digestion of fruits and vegetables?

Blender companies state that a blender for smoothies should have speeds of at least 30000 rpm. They argue that only at 30000 rpm are cells of fruits and vegetables sheared enough to maximize nutrition ...
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657 views

Are mature RBCs prokaryotic?

Mature mammalian RBCs have all the characteristics of a eukaryotic cell except that they don't have a nucleus, they don't have any cell organelles. Does this mean that RBCs are classified as ...
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188 views

Do any cells change in size or mass as mammals grow?

That is to say, are there cells that, between infancy and adulthood, get larger? Or is all growth done entirely via cell division? I'm wondering if it is safe to assume that the approximate number ...
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61 views

Plastid and mitochondria

I am not biologist, so please bear with me for this basic question. Although I tried googling, I am confused. What is difference between plastid, chloroplast and mitochondria? Are there any plant ...
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1answer
172 views

How can I create a microcapillary for manipulation of single cells?

i'm working as a diy bio. I'm finding a way to create a micro glass capillary for picking up single cells. I see this video on youtube and would like to know what is the minimum I/O diameter if I pull ...
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74 views

Is it possible to give a person an allergic reaction in a very specific place?

Can I give a person an allergic reaction at a very specific spot in a tissue? And if so, how accurate can i get?
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39 views

peptide MHC microarray

"The recent technology is peptide–MHC microarray or artificial antigen-presenting chip. In this technique, recombinant peptide–MHC complexes and co-stimulatory molecules are immobilized on a ...
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1answer
42 views

How to estimate the DNA density in human sperm head?

I have got an estimate of sperm head volume from internet.Like consider it as a disk of order 4$\mu$m to 5$\mu$m. Now I wanted to find the DNA density in the sperm head. How to find that?
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55 views

What is the functional significance of the difference in cardiolipin/cholesterol ratio in different membranes?

I have read somewhere that the plasma membrane has little cardiolipin but excess cholesterol whereas the inner mitochondrial membrane is rich in cardiolipin and has little cholesterol.I just wanted to ...
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50 views

Advantage of cup-like shape of blood cells, spores?

Mold spores sometimes have the same shape as platelets in blood. If I were designing a spore it would probably be spherical. Is there any advantage to this cup-like shape? Maybe there is some ...
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121 views

What does it mean for a chemical pathway to be conserved?

In many papers the MAPK pathway, (along with many others) is referred to as being conserved: Example: "The mitogen-activated protein kinase (MAPK) cascades are ubiquitous in eukaryotic signal ...
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58 views

Diffusion of FAD+

Why is NAD+ free to diffuse within the mitochondrion whereas FAD+ is not ? What biochemical properties cause this difference ?
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226 views

What is 'calcium conductance'?

What is the meaning of calcium conductance in ion channels. I encountered this in the following text: It was established that the µ and δ opioid receptors open potassium channels, which results ...
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116 views

Suggestions for an experiment? [closed]

I would appreciate suggestions for my year-long AP Biology project. Such a project would consists of a standard experiment, to be done over the period of a year, so it would have >to be fairly ...
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2answers
60 views

The role of antibodies interacting with cancer

I'm learning about antibodies. As I understand it, antibodies detect stranger cells/bacterial/viruses by the molecules present in their membranes. In cancer cells, the cancer cell have produce some ...
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83 views

What in soy are people allergic to and will these allergens be passed along if an animal that has eaten soy is consumed?

I have two related questions about soy allergies and possible reactions. What is it that are humans who have soy allergies actually allergic to? What is the allergen? If an animal eats soy, are ...
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197 views

Would two species of yeast with similar genome sizes have the same number of genes or chromosomes?

Similar organisms generally have similar genome sizes. Given this, would two species of yeast have the same number of genes and chromosomes? Edit: Fixed with thanks to @daniel-standage
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24 views

Complementarity Determining Regions (CDR)

Complementarity determining regions (CDRs) are part of the variable chains in immunoglobulins (antibodies) and T cell receptors, generated by B-cells and T-cells respectively, where these molecules ...
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1answer
34 views

How to measure the total number of cells in an average human body?

I have got one assignment to calculate the approximate sum total of all cells in the human body. How to tackle this problem? I know that the current statistics is $10^{13}$ cells. I wanted some hints. ...
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60 views

Consequence of touching Formaldehyde

I accidently touched formaldehyde some days ago. Skin on my hand got dehydrated as if I had placed it in salt solution. I washed it with water and it returned to normal state after 5 minutes. But now ...
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48 views

how cells make other macro molecules?

We know that the nucleus of the cell is the White House of the cell and its DNA is the president and it commands to make protein. So my question when DNA only codes for protein and enzymes,after the ...
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30 views

Dinuclei cellular mechanism

I am no biologist, but I have this question buzzing in my mind. It's a matter of curiosity What happens when two nuclei occupy the same cell? Would we expect the embryo (if it lives) to have complex ...
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1answer
56 views

Photosynthesis: Splitting Water

The splitting of water is an endergonic (non-spontaneous) reaction, and thus would require energy (chemical work to be done) in order to happen. In Photosystem II, an enzyme catalyzes this splitting, ...
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132 views

Characteristics of life

If all of the characteristics of life are required for something to be defined as living, how can we account people or other life that can not reproduce?
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48 views

Method to inject genes into cells

The famous Hershey and Chase Experiment uses phages to inject genes into cells. In the beginning, Professor Yamanaka injected 24 types of genes into cells to figure out which genes turn a cell into ...
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70 views

Botulinum Toxin

I asked on the cooking site, but was directed here. This is my original question http://cooking.stackexchange.com/questions/35726/botulinum-toxin-in-home-canned-green-beans The reading is quite ...
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1answer
92 views

What is background binding?

I'm reading a journal article about mitochondrial protein import and it mentions that one of the mutant proteins tested showed background binding with another protein. What the heck is background ...
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61 views

In a human, what non-germline cells have the highest/lowest mass?

I'm just curious which cells are largest/smallest in the human body other than sperm/ova.
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27 views

Determining and Differentiating specific ATPases

In respects to this certain ATPase transporter. What do i look for in respects to determining what specific kind it is out of: F-ATPases V-ATPases A-ATPases P-ATPases E-ATPases Im assuming that ...
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19 views

Microarray probe and target

In a microarray, which one is called a target and which one is the probe? the one that is added later , is that the probe or the one present in the slots of the microarray, that is the probe? (I have ...
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100 views

RNeasy Mini Kit low 260/230 ratio — can I purify this RNA for further use?

I used Qiagen's RNeasy Mini Kit to isolate RNA from 5*10^5 C28/I2 (immortalized human chondrocytes). However, my yield is low (~25 ng/ul), but my 260/280 ratio is great (~2.3), and my 260/230 ratio is ...