The study of cells: their physiological properties, structure, environmental interaction, division, life cycle, and death, as well as the organelles they contain. Also known as cytology.

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Estimating RPM to RCF in Methods from Older Papers

I'm attempting to replicate a cell biology method from a 1958 Laboratory Investigation paper. The protocol is for the isolation of an extracellular matrix protein, and a key step is a centrifugation ...
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30 views

Prevent biofilm formation on moist surfaces

I have an indoor fountain with lots of water. What are the most common microorganisms in this kind of moist environment, and what are the standard way to prevent biofilm formation by them? I thought ...
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Why do some internal organs regenerate?

I have been reading about the human liver and zebra fish heart muscle having the ability to regenerate. It seems to me that these organs have very little chance to become damaged or worn out. At the ...
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What metabolically happens when an egg fuses with the nucleus of a somatic cell

In stem cell biology, it is recognized that embryonic stem cells are transcriptionally inactive for the first 3 days of development. However, during somatic cell nuclear transfer, the nucleus is ...
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Single long axon vs serial neurons

Based on the comments in this post and also this chat. For discussions and speculations please comment in the chat. The basic question is what is the advantage of having a single long axon such as ...
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How many cells are there in the apical meristematic tissue?

How many cells are there in the apical meristematic tissue? Looking at this picture... , I would tend to think that there are few hundreds cells in the meristem tissue. But I guess this is a ...
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167 views

Can someone explain the color-changing unit (CCU) to me?

I've been physically carrying out serial tenfold dilutions on samples of Ureaplasma to work out the color-changing units (CCU). As a definition, the CCU is the highest dilution at which there is a ...
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A program for cell motility assessment with a batch process function?

Cell motility assessment is a branch of experimental biology or medical science. One example could be an assessment of treatment effects on sperm motility of an animal. The standard procedure involves ...
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69 views

Microscopy Book Suggestions

I've learned programming through great book recommendations, many from the Stack Exchange series of sites. I'm hoping to take this approach to gaining a fundamental understanding of how fluorescence ...
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49 views

Patch-Clamping Neurons Resources

Are there any lectures available on Patch-Clamping? Were can I find a mathematical model of Patch-Clamp? (that can be easily implemented in matlab). I have found some step-by-step protocols on ...
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How do I get recombinant proteins into the nucleus of mammal cells?

I know that there are Nuclear Localisation Sequencenes (NLS). They can be taken from endogenous or viral proteins and fused to the N or C terminus of my recombinant protein. Which is the best one? ...
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51 views

What proportion of salt in water would make it not dehydrate nor hydrate someone?

Drinking sea water can be deadly as it contains too much salt, basically de-hydrating the body. Normal tap water contains little salt and is good for re-hydration. My question: How much salt needs to ...
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152 views

Which of the cell types commonly found in mammals has the greatest number of mitochondria?

This is basically a fun question, inspired by this answer on scifi.se. So, which cell type will have the greatest number of mitochondria? Obviously, I am talking about wild type, healthy individuals ...
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204 views

Image Processing Suite for bacterial microscopy: Schnitzcells or MicrobeTracker?

I am looking to start doing some work tracking the size and growth of individual bacterial cells in the microscope. In order to analyze the images I need software that can segment the cells, ...
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57 views

Critical pO2 threshold for cell division?

I'm a physicist asking questions on an aspect of cellular metabolism, so excuse my biological ignorance! Hypoxia is a frequent complication of certain tumour types, and has quite an implication for ...
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110 views

Coiling of chromatids during cell division

What is exactly coiling of chromosomes? I just heard about the names i.e paranemic, plectonemic, orthostichious, anorthospiral. I have ecaxtly no idea of what phenomenon is this. Also what type of ...
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Example(s) of reduced rate of mitotic progression?

Most species complete mitosis, and in particular the process of chromosome condensation, rather quickly, in a matter of minutes. Are there any known species that undergo mitoses with substantially ...
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Onset of Autophagy

Fasting and Intermittent Fasting (IF) have been proven to start autophagy (cellular self-digestion). How long does the average man and woman have to wait for autophagy to begin? Does diet prior to ...
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Slicing cell cultures frozen in vitrified ice with a laser for CryoEM

Could a cell culture frozen in vitrified ice for CryoEM be effectively sliced into thin planes and resolved images produced? I was thinking if this could be done to get images from inside a cell.
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Alkaline Phosphatase

What is the predominant purpose of Alkaline Phosphatase in skeletal muscle fibers and liver cells. I know that it is a hydrolase enzyme that speeds up the degredation of proteins, lipids, starch and ...
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recombinant peptide MHC complex

What is recombinant peptide-MHC complex??? Recombinant DNA means "to bring together genetic material from multiple sources, creating sequences that would not otherwise be found in biological ...
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MAPK signalling pathway initial conditions

I would like to know the initial conditions and locations of the chemical reagents in the MAPK cascasde. I'm going to plug this information into a reaction diffusion simulation. From reading ...
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19 views

Why are embryonic germ cells considered stem cells?

In a class that I'm taking we were presented 3 types of stem cells. Adult stem cells which come from bone marrow Embryonic stem cells which come from embryos Embryonic germ cells which come from ...
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111 views

MTT assay normalization

Since the precise amount of cells in each well of an MTT assay varies, how can I normalize the results by cell number/concentration? How can I take into account the number of cells that have already ...
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37 views

Nearly Exhaustive List for Cholesterol Pathways

I have run across an interesting case that is similar to only two others I've encountered. What makes it interesting is the combination of undetectable (under normal testing conditions, can elaborate ...
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179 views

Microtiter Dish Biofilm Formation Assay- Pseudomonas and Crystal Violet

If Pseudomonas is a gram negative bacteria, it does not retain crystal violet but why is it that so many people are using crystal violet staining in theri Microtiter Dish Biofilm Formation Assay?
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Differences in Karr cell model and E-Cell

Recently Karr et al. published in Cell their model of Mycoplasma genitalium and published the complete code. Also, the E-Cell project allows to simulate a complete cell. Can anyone comment on ...
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19 views

Immunomic Microarray

"One can measure two or more signals simultaneously determined by a single feature, i.e., epitope in immunomic microarray DNA microarrays measure one response value for each gene per sample; that ...
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MHC restricted peptide

What is an MHC restricted peptide? "MHC-restricted antigen recognition, or MHC restriction, refers to the fact that a given T cell will recognize a peptide antigen only when it is bound to a host ...
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TAA- Tumor associated antigen

An approach to find Tumor associated antigens is based on transfection of expression library made from cDNA into cells expressing desired MHC haplotypes. Can someone please explain what this line ...
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33 views

How is the growth of benign tumors suppressed?

A benign tumor has an outer layer of cancerous cells beyond which are regular cells (I Think). The Tumor must have some kind of boundary layer like a wall where somehow the cancerous cells can't ...
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32 views

Cells and their characteristic properties

Could a culture of cells of a certain type be 'forced' to attain certain properties in successive generations that would have been harmful to the cell culture as they are now?
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Macrophage death by toxin. Little balls inside cell? What type of death?

After I treated J774 cells ( a mouse monocyte/macrophage cell line ) with Clostridium difficile Toxin B (1 ng/ml for 44 hours), I noticed several small circles inside of a seemingly dead cell. I'm ...
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Cell volumes of the MDCK, COS1 and ECV standard cell lines?

Where can I find information regarding their volumes? In general where can I find information regarding common cell types?