The study of cells: their physiological properties, structure, environmental interaction, division, life cycle, and death, as well as the organelles they contain. Also known as cytology.

learn more… | top users | synonyms

7
votes
0answers
86 views

Pancretic Acinar Cell - ATP, calcium concentration data

I need to find a decent source of data for concentration of ATP and calcium in the pancreatic acinar cell. So far all I can find is ATP or calcium 'levels' based on fluorescence , which are not ...
3
votes
0answers
133 views

Can someone explain the color-changing unit (CCU) to me?

I've been physically carrying out serial tenfold dilutions on samples of Ureaplasma to work out the color-changing units (CCU). As a definition, the CCU is the highest dilution at which there is a ...
3
votes
0answers
25 views

A program for cell motility assessment with a batch process function?

Cell motility assessment is a branch of experimental biology or medical science. One example could be an assessment of treatment effects on sperm motility of an animal. The standard procedure involves ...
3
votes
0answers
64 views

Microscopy Book Suggestions

I've learned programming through great book recommendations, many from the Stack Exchange series of sites. I'm hoping to take this approach to gaining a fundamental understanding of how fluorescence ...
3
votes
0answers
47 views

Patch-Clamping Neurons Resources

Are there any lectures available on Patch-Clamping? Were can I find a mathematical model of Patch-Clamp? (that can be easily implemented in matlab). I have found some step-by-step protocols on ...
3
votes
0answers
24 views

What metabolically happens when an egg fuses with the nucleus of a somatic cell

In stem cell biology, it is recognized that embryonic stem cells are transcriptionally inactive for the first 3 days of development. However, during somatic cell nuclear transfer, the nucleus is ...
2
votes
0answers
72 views

Which of the cell types commonly found in mammals has the greatest number of mitochondria?

This is basically a fun question, inspired by this answer on scifi.se. So, which cell type will have the greatest number of mitochondria? Obviously, I am talking about wild type, healthy individuals ...
2
votes
0answers
160 views

Image Processing Suite for bacterial microscopy: Schnitzcells or MicrobeTracker?

I am looking to start doing some work tracking the size and growth of individual bacterial cells in the microscope. In order to analyze the images I need software that can segment the cells, ...
2
votes
0answers
52 views

Critical pO2 threshold for cell division?

I'm a physicist asking questions on an aspect of cellular metabolism, so excuse my biological ignorance! Hypoxia is a frequent complication of certain tumour types, and has quite an implication for ...
2
votes
0answers
168 views

which exact mechanism triggers the first cell differentiation after n divisions?

I would like to understand which mechanism triggers the first cell differentiation after n divisions. I read previous articles on SE and Wikipedia articles on cellular differentiation and ...
2
votes
0answers
84 views

Coiling of chromatids during cell division

What is exactly coiling of chromosomes? I just heard about the names i.e paranemic, plectonemic, orthostichious, anorthospiral. I have ecaxtly no idea of what phenomenon is this. Also what type of ...
2
votes
0answers
47 views

How to use mechanical microstrainer to extract tissue proteins from human?

Background: There are many methods to extract proteins form human tissues out there. The majority of them use an extraction buffer containing variable concentrations of detergents and protease ...
2
votes
0answers
37 views

Example(s) of reduced rate of mitotic progression?

Most species complete mitosis, and in particular the process of chromosome condensation, rather quickly, in a matter of minutes. Are there any known species that undergo mitoses with substantially ...
2
votes
0answers
46 views

Onset of Autophagy

Fasting and Intermittent Fasting (IF) have been proven to start autophagy (cellular self-digestion). How long does the average man and woman have to wait for autophagy to begin? Does diet prior to ...
1
vote
0answers
18 views

Is there a classification of species based on number of different cell types?

The human body has around 200 different types of cells, according to my brief internet research. How does that compare to other species? Is there a classification of species based on that, i.e. number ...
1
vote
0answers
32 views

Has anyone used Crispr/Cas to induce a knock-in in MEF cells?

Does anyone have experience with the crispr/cas9 platform performed on MEF? Or does anyone recall any relevent articles? Thanks
1
vote
0answers
19 views

MAPK signalling pathway initial conditions

I would like to know the initial conditions and locations of the chemical reagents in the MAPK cascasde. I'm going to plug this information into a reaction diffusion simulation. From reading ...
1
vote
0answers
17 views

Why are embryonic germ cells considered stem cells?

In a class that I'm taking we were presented 3 types of stem cells. Adult stem cells which come from bone marrow Embryonic stem cells which come from embryos Embryonic germ cells which come from ...
1
vote
0answers
78 views

MTT assay normalization

Since the precise amount of cells in each well of an MTT assay varies, how can I normalize the results by cell number/concentration? How can I take into account the number of cells that have already ...
1
vote
0answers
28 views

Nearly Exhaustive List for Cholesterol Pathways

I have run across an interesting case that is similar to only two others I've encountered. What makes it interesting is the combination of undetectable (under normal testing conditions, can elaborate ...
1
vote
0answers
132 views

Microtiter Dish Biofilm Formation Assay- Pseudomonas and Crystal Violet

If Pseudomonas is a gram negative bacteria, it does not retain crystal violet but why is it that so many people are using crystal violet staining in theri Microtiter Dish Biofilm Formation Assay?
1
vote
0answers
36 views

Why must abortive transcription repeat itself a number of times?

Why must abortive transcription repeat itself a number of times before a stable DNA:RNA hybrid containing a transcript of 11 ribonucleotides is formed?
1
vote
0answers
67 views

When should endocytosis inhibitors be used in cell binding assays?

I'm beginning to do some cell-binding assays and I would like for my proteins to not be endocytosed by my mammalian cells. Typical suggestions are for the cells to be kept on ice and that the binding ...
1
vote
0answers
94 views

Properties and life cycle of chondrocytes and tenocytes

Questions regarding chondrocytes and tenocytes: Do these cells replicate, or are they regenerated from mature stem cells? How often are these cells regenerated? How long do they live? Do they ...
0
votes
0answers
17 views

What models are used for the different pathways of apoptosis?

We are trying to find references for experimental models of different pathways of apoptosis (caspase-dependent and -independent intrinsic apoptosis, death receptor type-1 and -2 mediated apoptosis, ...
0
votes
0answers
10 views

Cell volumes of the MDCK, COS1 and ECV standard cell lines?

Where can I find information regarding their volumes? In general where can I find information regarding common cell types?
0
votes
0answers
31 views

How is the growth of benign tumors suppressed?

A benign tumor has an outer layer of cancerous cells beyond which are regular cells (I Think). The Tumor must have some kind of boundary layer like a wall where somehow the cancerous cells can't ...
0
votes
0answers
31 views

Cells and their characteristic properties

Could a culture of cells of a certain type be 'forced' to attain certain properties in successive generations that would have been harmful to the cell culture as they are now?
0
votes
0answers
15 views

What are conditions for a membrane to be settled ? (centrifugation)

The cell membranes have 60% of proteins and 40% of lipids. And we know that medium density of proteins is 1,2 g/cm3 and the medium density of lipids is 0.92 g/cm3. How can we calculate the medium ...
0
votes
0answers
25 views

Macrophage death by toxin. Little balls inside cell? What type of death?

After I treated J774 cells ( a mouse monocyte/macrophage cell line ) with Clostridium difficile Toxin B (1 ng/ml for 44 hours), I noticed several small circles inside of a seemingly dead cell. I'm ...
0
votes
0answers
42 views

Ideal blood cell

I had been given an assignment to draw an ideal blood cell, a cell which can perform the functions of almost all the blood cells (like red blood cells, neutrophils, basophils, eosinophils and ...
0
votes
0answers
36 views

Fusion of neuron with a S phase cell

There was some experiment in which a S phase cell was fused with other cell and the other cell also began to replicate DNA. Would the same happen if fused with nerve cell ? Why or why not?
0
votes
0answers
44 views

Domains in cell membrane

How is movement of proteins and lipids between different domains of cell membrane prevented? Why is the noncytosolic layer not able to do lateral movements between domains but cytosolic layer is able ...
0
votes
0answers
39 views

Generic Abbreviation for Immunopanning?

This is actually something I've been wondering for a while. I've seen all kinds of short hand used outside of papers (IPn most commonly), but in publications I've not seen an abbreviation for ...