Known also as "cytology" is a scientific discipline that studies cells – their physiological properties, their structure, the organelles they contain, interactions with their environment, their life cycle, division and death.
7
votes
2answers
120 views
Does pheomelanin have a useful biological function?
Melanin is a natural pigment that is categorized into two main forms, eumelanin and pheomelanin. It's well documented in the science literature that increased eumelanin levels reduces the risk of ...
5
votes
1answer
106 views
Using ion-exchange chromatography to purify DNA from a cell extract - Is DNA more negatively charged then RNA?
When applying this method we have a glass or plastic column of resin which is positively charged. Then we pour cell extract into the column in order to capture the negatively charged particles which ...
5
votes
1answer
68 views
Number of spindle fibres during Metaphase?
During metaphase, the chromosomes are arranged on the equatorial plate and are attached to spindle fibres. After S phase, can the cell be said to attain the configuration of 4n?
Also, during ...
4
votes
1answer
53 views
Intrinsic apoptosis in erythrocytes
With a lack of mitochondria, can red blood cells perform intrinsic apoptosis and do they have another way of generating cytochrome c to attach to a CARD domain and assemble the apoptosome?
Or are ...
6
votes
0answers
51 views
Pancretic Acinar Cell - ATP, calcium concentration data
I need to find a decent source of data for concentration of ATP and calcium in the pancreatic acinar cell.
So far all I can find is ATP or calcium 'levels' based on fluorescence , which are not ...
5
votes
0answers
37 views
Primary cilia: what cell types have non-motile cilia that migrate?
My understanding is that there are two broad categories of cilia: motile and non-motile (also called primary.
Examples of the former include sperm flagella and the cilia of epithelial cells that ...
4
votes
0answers
52 views
Can NSAIDs impact negatively the healing of tendons?
There are a number of articles regarding NSAIDs having a negative effect on healing conditions like tendonosis and tendinitis. From what I understand the channel through which they reduce inflammation ...
3
votes
0answers
15 views
A program for cell motility assessment with a batch process function?
Cell motility assessment is a branch of experimental biology or medical science. One example could be an assessment of treatment effects on sperm motility of an animal. The standard procedure involves ...
3
votes
0answers
48 views
How does healing work?
I stacked my bike and split my head right open. About three weeks later the scab falls off and the skin is sealed over, like magic! How does it all work? How does the brain know that part of the ...
3
votes
0answers
43 views
Microscopy Book Suggestions
I've learned programming through great book recommendations, many from the Stack Exchange series of sites. I'm hoping to take this approach to gaining a fundamental understanding of how fluorescence ...
3
votes
0answers
28 views
Patch-Clamping Neurons Resources
Are there any lectures available on Patch-Clamping?
Were can I find a mathematical model of Patch-Clamp? (that can be easily implemented in matlab).
I have found some step-by-step protocols on ...
3
votes
0answers
19 views
What metabolically happens when an egg fuses with the nucleus of a somatic cell
In stem cell biology, it is recognized that embryonic stem cells are transcriptionally inactive for the first 3 days of development. However, during somatic cell nuclear transfer, the nucleus is ...
2
votes
0answers
35 views
Why is saltatory conduction faster than continuous conduction?
How does spacing apart sodium and potassium channels allow the action potential to travel faster down the axon? This is the reason always cited for saltatory conduction and myelination, but my mental ...
2
votes
0answers
28 views
Example(s) of reduced rate of mitotic progression?
Most species complete mitosis, and in particular the process of chromosome condensation, rather quickly, in a matter of minutes. Are there any known species that undergo mitoses with substantially ...
2
votes
0answers
32 views
Onset of Autophagy
Fasting and Intermittent Fasting (IF) have been proven to start autophagy (cellular self-digestion). How long does the average man and woman have to wait for autophagy to begin? Does diet prior to ...
1
vote
0answers
9 views
How to use mechanical microstrainer to extract tissue proteins from human?
Background:
There are many methods to extract proteins form human tissues out there. The majority of them use an extraction buffer containing variable concentrations of detergents and protease ...
1
vote
0answers
50 views
When should endocytosis inhibitors be used in cell binding assays?
I'm beginning to do some cell-binding assays and I would like for my proteins to not be endocytosed by my mammalian cells. Typical suggestions are for the cells to be kept on ice and that the binding ...
1
vote
0answers
52 views
Properties and life cycle of chondrocytes and tenocytes
Questions regarding chondrocytes and tenocytes:
Do these cells replicate, or are they regenerated from mature stem cells?
How often are these cells regenerated?
How long do they live?
Do they ...
