The study of cells, their physiological properties, structure, environmental interaction, division, life cycle, and death, as well as the organelles they contain. Also known as cytology.

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Contact Inhibition of Cell Division: Signaling Pathway

The following article refers to contact inhibition of cell division in epithelial cells, specifically MDCK cells: Collective and single cell behavior in epithelial contact inhibition. In their review ...
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215 views

Is there a karyotype database for human cell lines?

I'm looking for the karyotype of a specific cell line. Some karyotype are well known, such as HeLa or some carcinomas, but some are very hard to find, such as LG2 (B cells). Is there a database ...
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Why do kupffer cells not attack sporozoites of malaria?

During malaria, why don't kuppfer cells (hepatic macrophages) attack the plasmodium and stop schizogony, thus saving us from the disease?
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74 views

What is the essence of difference of how different chemicals affect the same receptor?

It is known that various chemicals can bind to the same receptor type, producing different effects. Be these chemicals agonists or antagonists, there are more variations in how they influence the ...
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39 views

What metabolically happens when an egg fuses with the nucleus of a somatic cell

In stem cell biology, it is recognized that embryonic stem cells are transcriptionally inactive for the first 3 days of development. However, during somatic cell nuclear transfer, the nucleus is ...
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38 views

What is the name of the category of viruses that affect only one side of the body?

The Varicella zoster virus causes chickenpox in children and shingles in adults. When the virus attacks as shingles, one of its distinguishing characteristics is that it only affects one side of the ...
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57 views

Is it possible to isolate cells harboring a specific DNA sequence?

Suppose on cell subpopulation harbours within its genome a specific DNA sequence that do not exist in other cell subpopulations. Is it technically possible to isolate such subpopulation ? and if yes, ...
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52 views

What are the sizes of the cells that make up human hair?

The question is in the title, but I'll explain why the question arose. I'm curious about the rates that various cells in the body divide, and have found various information relating to this, but ...
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90 views

L-tryptophan in mammalian cell culture

Why must the concentration of L-tryptophan be kept to a relatively low level when culturing mammalian cells? It's an essential amino acid, so I cannot fathom why low concentrations as opposed to ...
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58 views

What Chemical Trigger Causes Ectomycorrhiza To Change From Asexual To Sexual?

I want to know the trigger behind the change of asexual to sexual ectomycorrhiza when symbiosis with a tree root is formed. As ectomycorrhiza attaches itself to a root, it forms a relationship wherein ...
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142 views

Can I leave BL21(DE3) cells in room temperature?

I am preparing competent cells, and I finished inoculating a single colony in SOB. It has been incubating at 37 degrees Celsius for almost 16 hours since, and it's getting very late where I live. I ...
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28 views

Why do flagella form a bundle only when they rotate counterclockwise during chemotaxis?

During Chemotaxis in bacteria with flagella, the flagellar rotation dictates how the cell moves. If the flagella rotate counterclockwise, then they form a bundle at one end of the cell (---O) and ...
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218 views

How to prevent e coli from clumping (for FACS)?

I'm performing FACS on e coli, but the cells are clumping together so each event is multiple cells. I ran a control where I had one flask of e coli expressing GFP, and one flask expressing RFP. Run ...
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54 views

How many cells are there in the apical meristematic tissue?

How many cells are there in the apical meristematic tissue? Looking at this picture... , I would tend to think that there are few hundreds cells in the meristem tissue. But I guess this is a ...
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350 views

Can someone explain the color-changing unit (CCU) to me?

I've been physically carrying out serial tenfold dilutions on samples of Ureaplasma to work out the color-changing units (CCU). As a definition, the CCU is the highest dilution at which there is a ...
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40 views

A program for cell motility assessment with a batch process function?

Cell motility assessment is a branch of experimental biology or medical science. One example could be an assessment of treatment effects on sperm motility of an animal. The standard procedure involves ...
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78 views

Microscopy Book Suggestions

I've learned programming through great book recommendations, many from the Stack Exchange series of sites. I'm hoping to take this approach to gaining a fundamental understanding of how fluorescence ...
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43 views

Are all the atoms in your body replaced with others over a 30 year period?

I was reading Creation - Life and How to Make It by Steve Grand. (This is the book that inspired Jeff Bezos to start the AWS initiative. ) In it he makes the following statement: Anyhow, by now I ...
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34 views

How do human cells respond to mechanical pressure such as heel contact during walking?

Basically, I'd like to do a full accounting (inventory, assets, liabilities) of a human cell under mechanical pressure. For example, if in steady-state, does the cell consume more or less energy ...
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29 views

What's the purpose of Cdk activity having more than one method of becoming inactive?

Cdk becomes partially active once its bound to cyclin and then gets phosphorylated and fully active once a Cdk-activating kinase (CAK) phosphorylates the partially active Cdk. This fully activated Cdk ...
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33 views

How is flagellar movement controlled?

How does a cell control the movement of flagella? Both the rotatory movements and sideways movements helps the cell to move. How are these flagellar movements controlled ? It is through the regulation ...
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36 views

Can ' functional' stress 'forced upon' a cell actually cause cell mutations when they divide and reproduce?

If a group of cells are forced to go beyond their regular biological parameters that they should be under given ' healthy' functioning and while existing in these stressed states some of the cells ...
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26 views

Can mutation rate be increased for instance by mutagenesis targeting the DNA polymerase?

I am studying a non-cultivable bacterium living in an insect host, and I would like to generate some random mutants of this bacteria while preserving the insect. I am wondering if one way to do so ...
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56 views

What is NK-cell compartments?

with respect to the paper: Adaptive reconfiguration of the human NK-cell compartment in response to cytomegalovirus: A different perspective of the host-pathogen interaction What is meant by ...
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43 views

How do I get recombinant proteins into the nucleus of mammal cells?

I know that there are Nuclear Localisation Sequencenes (NLS). They can be taken from endogenous or viral proteins and fused to the N or C terminus of my recombinant protein. Which is the best one? ...
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318 views

MTT assay normalization

Since the precise amount of cells in each well of an MTT assay varies, how can I normalize the results by cell number/concentration? How can I take into account the number of cells that have already ...
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358 views

Image Processing Suite for bacterial microscopy: Schnitzcells or MicrobeTracker?

I am looking to start doing some work tracking the size and growth of individual bacterial cells in the microscope. In order to analyze the images I need software that can segment the cells, ...
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74 views

Critical pO2 threshold for cell division?

I'm a physicist asking questions on an aspect of cellular metabolism, so excuse my biological ignorance! Hypoxia is a frequent complication of certain tumour types, and has quite an implication for ...
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40 views

Example(s) of reduced rate of mitotic progression?

Most species complete mitosis, and in particular the process of chromosome condensation, rather quickly, in a matter of minutes. Are there any known species that undergo mitoses with substantially ...
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56 views

Onset of Autophagy

Fasting and Intermittent Fasting (IF) have been proven to start autophagy (cellular self-digestion). How long does the average man and woman have to wait for autophagy to begin? Does diet prior to ...
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20 views

Barcode exchange between neighbouring cells

I am currently interested in equipping animal cells in a tissue with individual barcodes. These barcodes should get amplified in the cells (not to a cell degrading extent, of course), and also ...
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7 views

Is EGF-mediated proliferation symmetric?

It's known that the MAPK signaling cascade (say, ERK) is downstream of EGFR in epithelial cells, and that EGFR activation can cause proliferation in some cell types in some situations. My question is ...
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8 views

HSC Cycling Rates

I would like to know how often human hematopoietic stem cells go into cycle in the bone marrow niche (with a paper reference). I have heard they cycle 1-2 times per year but has anyone robustly ...
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25 views

mRNA extraction from mice ears.

I am trying to extract RNA from mice ears and for some reason I don't have RNA when I perform the electrophoresis. I directly cut the ears and I put it in a tube with a bead and trizol. then I place ...
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31 views

Bacteria surviving a β-lactam antibiotic

What changes can occur in the cell wall of a bacteria for it to survive a β-lactam antibiotic? I think that because a bacteria possesses peptidoglycan in its cell wall, they are β-lactam sensitive, ...
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17 views

Ions in different cell compartments

I would like to understand the distribution of metal ion concentrations (such as Mg2+) in the different cell compartments. I tried very hard but still couldn't find such information. Could anyone ...
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23 views

Degenerate primer designing software

Can any one provide online free degenerate primer designing software. I have tried couple of them like CODEHOP is one where BLOCK formatting step finding difficulty, can anyone help me out....
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35 views

How do cell repair mechanism ratios change as they age?

I have seen that embryonic stem cells are shown to use homologous repair for double strand breaks rather then non-homologous end joining (NHEJ). [1] I am wondering if something also happens to a ...
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23 views

Could drugs promoting angiogenesis be used as a treatment for burn victims?

From what I've learned from my textbooks and in class lecutures it seems that inducing angiogenesis for people with severe burns would be an excellent way to speed up the healing process. Is this ...
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30 views

Why don't the minus ends of microtubules shrink?

Is this because $\alpha$-tubulin is always in the GTP state? Or is it because $\gamma$-TuRC stabilizes the minus end?
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39 views

Mechanism of Octoxynol-10 as a preservative in vaccines

I noticed that the Fluarix Quad flu shot this season contains Octoxynol-10 rather than Thimerosal as a preservative. I am not expert in this area, so I did a Google search of "Octoxynol-10", and ...
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20 views

What is the significance of 9+2 arrangement in flagella?

Why eukaryotic flagella have 9+2 arrangement .? What is the significance? a 9+0 flagella should have worked the same way .
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16 views

Growth of Yeast in Different pH Mediums

Will yeast grow better in acidic, basic or neutral mediums? Why? Will the medium affect the growth of yeast? (Yeast cell membrane is semi-permeable).
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331 views

Why do some white blood cells have lobed nuclei?

Several types of white blood cells (eg Neutrophils) have lobed nuclei. Is this for a functional reason? I have seen people refer to structural differences in the lobes as indicative of problems, but ...
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28 views

Is a Ribonuclease Inhibitor protein the only mechanism employed by the human cell to protect cellular RNA from degradation by endoribonuclease?

Are there any other methods of protection for cellular RNA from degradation by endonuclease (specifically the Pancreatic Ribonuclease RNase II (Eosinophil-derived neurotoxin) and RNase III (Eosinophil ...
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25 views

Are there any auto-immune diseases caused by T cells not detaching from antigen presenting cells (APCs)?

By not detaching I'm referring to after they have formed an immunological synapse, if they don't ever detach.
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Genetic tests on S. cerevisiae to determine mutation locus on genes

I am studying the metabolism of galactose in the budding yeast Saccharomyces cerevisiae. After a random mutagenesis screen, several mutant strains were isolated that grow well in glucose but are ...
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38 views

What is a “NuMA protein”?

I just got out of a lecture on mitosis and this part was incredibly confusing. After searching my textbook it doesn't cover it that well either. The professor mentioned a NUMA protein that is ...
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52 views

Question about cytoskeleton coordination

I am trying to study for a biology and I am having some confusion over the following topic. Can anyone help explain/ shed some light on the concepts of Rho family GTPases. Is it true that we have Rho ...
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What kind of experiments could you do to see which end of a microtubule is most dynamic?

I'm stuck between two ideas. Not sure which one of them would be best. Do a FRAP on the middle of a MT. If it grows from the + end, that section will remain dark. If it grows from the - end, ...