Tagged Questions
3
votes
1answer
24 views
How do different tissue culture matrices affect background in fluorescent microscopy?
In response to my previous question, I've been reading up a little bit on poly-D-lysine, Collagen I, Collagen IV, laminin, and other tissue culture coatings that promote cell adhesion. I've always ...
2
votes
1answer
54 views
Copper for cell incubator to prevent contamination
For some reason the lab seems to have a problem with contamination every so often. It's virtually impossible to prevent bacteria, viruses, fungi, etc. from getting into the incubator every time you ...
3
votes
2answers
157 views
How can I resuspend a cell pellet without harming the bacteria?
When using a preculture with Ampicillin in my protein expression, I have to get rid of the preculture medium to avoid carrying over too much beta-lactamases that will destroy the ampicillin in the ...
2
votes
0answers
44 views
Resuspending Cells from a filter plate
My experiment involves pulling down cells on to a filter plate for a variety of assays. However, I want to ensure that my cells are alive so I am trying to resuspend them so that I can do an accurate ...
