Cell culture is the process of growing and maintaining living cells in vitro.

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Appropriate methods for triteration? [closed]

What is the appropriate method to pipetting up and down mammalians cells to sufficiently break up clumps. This should also keep in mind the issues from Pipetting damage on cells. Visually one can see ...
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2answers
138 views

How is it that the WI-38 cell line isolated by Hayflick in 1962 is still very much around and not affected by the 'Hayflick Limit'?

I have searched the net and I have not been able to come up with an clear answer. Edit: Here is the para quoted from Nature http://www.nature.com/news/medical-research-cell-division-1.13273 "So began ...
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1answer
48 views

Looking for raw cell videos from a light microscope

First off, I'm not looking for online lectures. I am trying to find creative commons licensed videos of live cells moving around without annotation or anything extra. I would prefer a few minutes of ...
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15 views

Cells scatter light in solution. Do they refract?

For part of an exam question, "why do cells grown in liquid cause turbidity?" I answered that cells refract light. The correct answer was that cells scatter light. Isn't the light scattering ...
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32 views

Cells and their characteristic properties

Could a culture of cells of a certain type be 'forced' to attain certain properties in successive generations that would have been harmful to the cell culture as they are now?
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27 views

Cells “grown in LB broth to an OD600nm” [closed]

Cells "grown in LB broth to an OD600nm" What does the OD stand for in this?
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73 views

Henrietta Lacks and HeLa cells

Why did Henrietta Lacks die when her cells didn't die? As I understand it only the cells from her cervix were immortal so perhaps the cervical cancer took her life and the cells never died.